Cloning and Characterization of R3b; Members of the R3 Superfamily of Late Blight Resistance Genes Show Sequence and Functional Divergence

G.C. Li, S.W. Huang, X. Guo, Y. Li, Y. Yang, Z. Guo, H.H. Kuang, H. Rietman, J.E.M. Bergervoet-van Deelen, V.V.G.A. Vleeshouwers, E.A.G. van der Vossen, D.Y. Qu, R.G.F. Visser, E. Jacobsen, J.H. Vossen

Research output: Contribution to journalArticleAcademicpeer-review

59 Citations (Scopus)

Abstract

Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacking, because it contains two closely linked R genes (R3a and R3b) with distinct resistance specificities to Phytophthora infestans. Here, we report about the positional cloning of R3b. Both transient and stable transformations of susceptible tobacco and potato plants showed that R3b conferred full resistance to incompatible P infestans isolates. R3b encodes a coiled-coil nucleotide-binding site leucine-rich repeat protein and exhibits 82% nucleotide identity with R3a located in the same R3 cluster. The R3b gene specifically recognizes Avr3b, a newly identified avirulence factor from P. infestans. R3b does not recognize Avr3a, the corresponding avirulence gene for R3a, showing that, despite their high sequence similarity, R3b and R3a have clearly distinct recognition specificities. In addition to the Rpi-mcd1/Rpi-blb3 locus on chromosome IV, the R3 locus on chromosome XI is the second example of an R-gene cluster with multiple genes recognizing different races of P. infestans.
Original languageEnglish
Pages (from-to)1132-1142
JournalMolecular Plant-Microbe Interactions
Volume24
Issue number10
DOIs
Publication statusPublished - 2011

Fingerprint

vpr Genes
Phytophthora infestans
Organism Cloning
molecular cloning
Solanum tuberosum
Chromosomes
Genes
genes
Nucleotides
chromosomes
loci
Multigene Family
nucleotides
potatoes
Tobacco
Breeding
Binding Sites
multigene family
leucine
binding sites

Keywords

  • broad-spectrum resistance
  • potato solanum-tuberosum
  • race-specific resistance
  • phytophthora-infestans
  • disease resistance
  • confers resistance
  • cultivated potato
  • plant
  • locus
  • bulbocastanum

Cite this

Li, G.C. ; Huang, S.W. ; Guo, X. ; Li, Y. ; Yang, Y. ; Guo, Z. ; Kuang, H.H. ; Rietman, H. ; Bergervoet-van Deelen, J.E.M. ; Vleeshouwers, V.V.G.A. ; van der Vossen, E.A.G. ; Qu, D.Y. ; Visser, R.G.F. ; Jacobsen, E. ; Vossen, J.H. / Cloning and Characterization of R3b; Members of the R3 Superfamily of Late Blight Resistance Genes Show Sequence and Functional Divergence. In: Molecular Plant-Microbe Interactions. 2011 ; Vol. 24, No. 10. pp. 1132-1142.
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abstract = "Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacking, because it contains two closely linked R genes (R3a and R3b) with distinct resistance specificities to Phytophthora infestans. Here, we report about the positional cloning of R3b. Both transient and stable transformations of susceptible tobacco and potato plants showed that R3b conferred full resistance to incompatible P infestans isolates. R3b encodes a coiled-coil nucleotide-binding site leucine-rich repeat protein and exhibits 82{\%} nucleotide identity with R3a located in the same R3 cluster. The R3b gene specifically recognizes Avr3b, a newly identified avirulence factor from P. infestans. R3b does not recognize Avr3a, the corresponding avirulence gene for R3a, showing that, despite their high sequence similarity, R3b and R3a have clearly distinct recognition specificities. In addition to the Rpi-mcd1/Rpi-blb3 locus on chromosome IV, the R3 locus on chromosome XI is the second example of an R-gene cluster with multiple genes recognizing different races of P. infestans.",
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author = "G.C. Li and S.W. Huang and X. Guo and Y. Li and Y. Yang and Z. Guo and H.H. Kuang and H. Rietman and {Bergervoet-van Deelen}, J.E.M. and V.V.G.A. Vleeshouwers and {van der Vossen}, E.A.G. and D.Y. Qu and R.G.F. Visser and E. Jacobsen and J.H. Vossen",
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Cloning and Characterization of R3b; Members of the R3 Superfamily of Late Blight Resistance Genes Show Sequence and Functional Divergence. / Li, G.C.; Huang, S.W.; Guo, X.; Li, Y.; Yang, Y.; Guo, Z.; Kuang, H.H.; Rietman, H.; Bergervoet-van Deelen, J.E.M.; Vleeshouwers, V.V.G.A.; van der Vossen, E.A.G.; Qu, D.Y.; Visser, R.G.F.; Jacobsen, E.; Vossen, J.H.

In: Molecular Plant-Microbe Interactions, Vol. 24, No. 10, 2011, p. 1132-1142.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Cloning and Characterization of R3b; Members of the R3 Superfamily of Late Blight Resistance Genes Show Sequence and Functional Divergence

AU - Li, G.C.

AU - Huang, S.W.

AU - Guo, X.

AU - Li, Y.

AU - Yang, Y.

AU - Guo, Z.

AU - Kuang, H.H.

AU - Rietman, H.

AU - Bergervoet-van Deelen, J.E.M.

AU - Vleeshouwers, V.V.G.A.

AU - van der Vossen, E.A.G.

AU - Qu, D.Y.

AU - Visser, R.G.F.

AU - Jacobsen, E.

AU - Vossen, J.H.

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N2 - Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacking, because it contains two closely linked R genes (R3a and R3b) with distinct resistance specificities to Phytophthora infestans. Here, we report about the positional cloning of R3b. Both transient and stable transformations of susceptible tobacco and potato plants showed that R3b conferred full resistance to incompatible P infestans isolates. R3b encodes a coiled-coil nucleotide-binding site leucine-rich repeat protein and exhibits 82% nucleotide identity with R3a located in the same R3 cluster. The R3b gene specifically recognizes Avr3b, a newly identified avirulence factor from P. infestans. R3b does not recognize Avr3a, the corresponding avirulence gene for R3a, showing that, despite their high sequence similarity, R3b and R3a have clearly distinct recognition specificities. In addition to the Rpi-mcd1/Rpi-blb3 locus on chromosome IV, the R3 locus on chromosome XI is the second example of an R-gene cluster with multiple genes recognizing different races of P. infestans.

AB - Massive resistance (R) gene stacking is considered to be one of the most promising approaches to provide durable resistance to potato late blight for both conventional and genetically modified breeding strategies. The R3 complex locus on chromosome XI in potato is an example of natural R gene stacking, because it contains two closely linked R genes (R3a and R3b) with distinct resistance specificities to Phytophthora infestans. Here, we report about the positional cloning of R3b. Both transient and stable transformations of susceptible tobacco and potato plants showed that R3b conferred full resistance to incompatible P infestans isolates. R3b encodes a coiled-coil nucleotide-binding site leucine-rich repeat protein and exhibits 82% nucleotide identity with R3a located in the same R3 cluster. The R3b gene specifically recognizes Avr3b, a newly identified avirulence factor from P. infestans. R3b does not recognize Avr3a, the corresponding avirulence gene for R3a, showing that, despite their high sequence similarity, R3b and R3a have clearly distinct recognition specificities. In addition to the Rpi-mcd1/Rpi-blb3 locus on chromosome IV, the R3 locus on chromosome XI is the second example of an R-gene cluster with multiple genes recognizing different races of P. infestans.

KW - broad-spectrum resistance

KW - potato solanum-tuberosum

KW - race-specific resistance

KW - phytophthora-infestans

KW - disease resistance

KW - confers resistance

KW - cultivated potato

KW - plant

KW - locus

KW - bulbocastanum

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DO - 10.1094/MPMI-11-10-0276

M3 - Article

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EP - 1142

JO - Molecular Plant-Microbe Interactions

JF - Molecular Plant-Microbe Interactions

SN - 0894-0282

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