CLASP stabilization of plus ends created by severing promotes microtubule creation and reorientation

Jelmer J. Lindeboom, Masayoshi Nakamura, Marco Saltini, Anneke Hibbel, Ankit Walia, Tijs Ketelaar, Anne Mie C. Emons, John C. Sedbrook, Viktor Kirik, Bela M. Mulder, David W. Ehrhardt*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

Central to the building and reorganizing cytoskeletal arrays is creation of new polymers. Although nucleation has been the major focus of study for microtubule generation, severing has been proposed as an alternative mechanism to create new polymers, a mechanism recently shown to drive the reorientation of cortical arrays of higher plants in response to blue light perception. Severing produces new plus ends behind the stabilizing GTP-cap. An important and unanswered question is how these ends are stabilized in vivo to promote net microtubule generation. Here we identify the conserved protein CLASP as a potent stabilizer of new plus ends created by katanin severing in plant cells. Clasp mutants are defective in cortical array reorientation. In these mutants, both rescue of shrinking plus ends and the stabilization of plus ends immediately after severing are reduced. Computational modeling reveals that it is the specific stabilization of severed ends that best explains CLASP's function in promoting microtubule amplification by severing and array reorientation.

Original languageEnglish
Pages (from-to)190-205
JournalThe Journal of cell biology
Volume218
Issue number1
DOIs
Publication statusPublished - 7 Jan 2019

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Microtubules
Polymers
Plant Cells
Guanosine Triphosphate
Light
Proteins
Drive
katanin

Cite this

Lindeboom, Jelmer J. ; Nakamura, Masayoshi ; Saltini, Marco ; Hibbel, Anneke ; Walia, Ankit ; Ketelaar, Tijs ; Emons, Anne Mie C. ; Sedbrook, John C. ; Kirik, Viktor ; Mulder, Bela M. ; Ehrhardt, David W. / CLASP stabilization of plus ends created by severing promotes microtubule creation and reorientation. In: The Journal of cell biology. 2019 ; Vol. 218, No. 1. pp. 190-205.
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title = "CLASP stabilization of plus ends created by severing promotes microtubule creation and reorientation",
abstract = "Central to the building and reorganizing cytoskeletal arrays is creation of new polymers. Although nucleation has been the major focus of study for microtubule generation, severing has been proposed as an alternative mechanism to create new polymers, a mechanism recently shown to drive the reorientation of cortical arrays of higher plants in response to blue light perception. Severing produces new plus ends behind the stabilizing GTP-cap. An important and unanswered question is how these ends are stabilized in vivo to promote net microtubule generation. Here we identify the conserved protein CLASP as a potent stabilizer of new plus ends created by katanin severing in plant cells. Clasp mutants are defective in cortical array reorientation. In these mutants, both rescue of shrinking plus ends and the stabilization of plus ends immediately after severing are reduced. Computational modeling reveals that it is the specific stabilization of severed ends that best explains CLASP's function in promoting microtubule amplification by severing and array reorientation.",
author = "Lindeboom, {Jelmer J.} and Masayoshi Nakamura and Marco Saltini and Anneke Hibbel and Ankit Walia and Tijs Ketelaar and Emons, {Anne Mie C.} and Sedbrook, {John C.} and Viktor Kirik and Mulder, {Bela M.} and Ehrhardt, {David W.}",
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doi = "10.1083/jcb.201805047",
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Lindeboom, JJ, Nakamura, M, Saltini, M, Hibbel, A, Walia, A, Ketelaar, T, Emons, AMC, Sedbrook, JC, Kirik, V, Mulder, BM & Ehrhardt, DW 2019, 'CLASP stabilization of plus ends created by severing promotes microtubule creation and reorientation', The Journal of cell biology, vol. 218, no. 1, pp. 190-205. https://doi.org/10.1083/jcb.201805047

CLASP stabilization of plus ends created by severing promotes microtubule creation and reorientation. / Lindeboom, Jelmer J.; Nakamura, Masayoshi; Saltini, Marco; Hibbel, Anneke; Walia, Ankit; Ketelaar, Tijs; Emons, Anne Mie C.; Sedbrook, John C.; Kirik, Viktor; Mulder, Bela M.; Ehrhardt, David W.

In: The Journal of cell biology, Vol. 218, No. 1, 07.01.2019, p. 190-205.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - CLASP stabilization of plus ends created by severing promotes microtubule creation and reorientation

AU - Lindeboom, Jelmer J.

AU - Nakamura, Masayoshi

AU - Saltini, Marco

AU - Hibbel, Anneke

AU - Walia, Ankit

AU - Ketelaar, Tijs

AU - Emons, Anne Mie C.

AU - Sedbrook, John C.

AU - Kirik, Viktor

AU - Mulder, Bela M.

AU - Ehrhardt, David W.

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AB - Central to the building and reorganizing cytoskeletal arrays is creation of new polymers. Although nucleation has been the major focus of study for microtubule generation, severing has been proposed as an alternative mechanism to create new polymers, a mechanism recently shown to drive the reorientation of cortical arrays of higher plants in response to blue light perception. Severing produces new plus ends behind the stabilizing GTP-cap. An important and unanswered question is how these ends are stabilized in vivo to promote net microtubule generation. Here we identify the conserved protein CLASP as a potent stabilizer of new plus ends created by katanin severing in plant cells. Clasp mutants are defective in cortical array reorientation. In these mutants, both rescue of shrinking plus ends and the stabilization of plus ends immediately after severing are reduced. Computational modeling reveals that it is the specific stabilization of severed ends that best explains CLASP's function in promoting microtubule amplification by severing and array reorientation.

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