Citrate, low pH and amino acid limitation induce citrate utilization in Lactococcus lactis biovar diacetylactis

Oscar van Mastrigt, Emma E. Mager, Casper Jamin, Tjakko Abee, Eddy J. Smid*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

In Lactococcus lactis subsp. lactis biovar diacetylactis, citrate transport is facilitated by the plasmid-encoded citrate permease (CitP). In this work, we analysed regulation of citrate utilization by pH, nutrient limitation and the presence of citrate at four different levels: (i) plasmid copy number, (ii) citP transcription, (iii) citP mRNA processing and (iv) citrate utilization capacity. Citrate was supplied as cosubstrate together with lactose. The citP gene is known to be induced in cells grown at low pH. However, we demonstrated that transcription of citP was even higher in the presence of citrate (3.8-fold compared with 2.0-fold). The effect of citrate has been overlooked by other researchers because they determined the effect of citrate using M17 medium, which already contains 0.80 ± 0.07 mM citrate. The plasmid copy number increased in cells grown under amino acid limitation (1.6-fold) and/or at low pH (1.4-fold). No significant differences in citP mRNA processing were found. Citrate utilization rates increased from approximately 1 to 65 μmol min−1 gDW−1 from lowest to highest citP expression. Acetoin formation increased during growth in an acidic environment due to induction of the acetoin pathway. Quantification of the relative contributions allowed us to construct a model for regulation of citrate utilization in L. lactis biovar diacetylactis. This knowledge will help to select conditions to improve flavour formation from citrate.
Original languageEnglish
Pages (from-to)369-380
JournalMicrobial Biotechnology
Volume11
Issue number2
DOIs
Publication statusPublished - 1 Mar 2018

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Lactococcus lactis
Transcription
Citric Acid
Amino acids
Cells
Amino Acids
Flavors
Processing
Nutrients
Genes
Acetoin
Plasmids
Messenger RNA
Membrane Transport Proteins
Lactose

Cite this

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title = "Citrate, low pH and amino acid limitation induce citrate utilization in Lactococcus lactis biovar diacetylactis",
abstract = "In Lactococcus lactis subsp. lactis biovar diacetylactis, citrate transport is facilitated by the plasmid-encoded citrate permease (CitP). In this work, we analysed regulation of citrate utilization by pH, nutrient limitation and the presence of citrate at four different levels: (i) plasmid copy number, (ii) citP transcription, (iii) citP mRNA processing and (iv) citrate utilization capacity. Citrate was supplied as cosubstrate together with lactose. The citP gene is known to be induced in cells grown at low pH. However, we demonstrated that transcription of citP was even higher in the presence of citrate (3.8-fold compared with 2.0-fold). The effect of citrate has been overlooked by other researchers because they determined the effect of citrate using M17 medium, which already contains 0.80 ± 0.07 mM citrate. The plasmid copy number increased in cells grown under amino acid limitation (1.6-fold) and/or at low pH (1.4-fold). No significant differences in citP mRNA processing were found. Citrate utilization rates increased from approximately 1 to 65 μmol min−1 gDW−1 from lowest to highest citP expression. Acetoin formation increased during growth in an acidic environment due to induction of the acetoin pathway. Quantification of the relative contributions allowed us to construct a model for regulation of citrate utilization in L. lactis biovar diacetylactis. This knowledge will help to select conditions to improve flavour formation from citrate.",
author = "{van Mastrigt}, Oscar and Mager, {Emma E.} and Casper Jamin and Tjakko Abee and Smid, {Eddy J.}",
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Citrate, low pH and amino acid limitation induce citrate utilization in Lactococcus lactis biovar diacetylactis. / van Mastrigt, Oscar; Mager, Emma E.; Jamin, Casper; Abee, Tjakko; Smid, Eddy J.

In: Microbial Biotechnology, Vol. 11, No. 2, 01.03.2018, p. 369-380.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Citrate, low pH and amino acid limitation induce citrate utilization in Lactococcus lactis biovar diacetylactis

AU - van Mastrigt, Oscar

AU - Mager, Emma E.

AU - Jamin, Casper

AU - Abee, Tjakko

AU - Smid, Eddy J.

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