Cisgenic Approach for improved disease resistance in apple

G.A.L. Broggini, C.E. Durel, E. Vergne, E. Chevreau, J. Fahrentrapp, T. Vanblaere, A. Peil, H. Flachowsky, M.V. Hanke, F.A. Krens, H.J. Schouten, C. Gessler

Research output: Chapter in Book/Report/Conference proceedingConference paper

Abstract

Swiss and more generally European apple (Malus × domestica) production is hampered by several diseases, the most destructive being fire blight, caused by Erwinia amylovora. On the other hand, there are apple scab, caused by Venturia inaequalis and powdery mildew, caused by Podosphaera leucotricha, which represent the major phytosanitary problems. Classical breeding has produced many scab and mildew resistant cultivars and efforts to breed also fire blight resistant cultivars are currently undertaken. Marker assisted selection (MAS) increases efficiency by allowing early non-destructive screening of seedlings and identifying genotypes showing pyramids of resistance genes. If the development of markers for MAS was the primary goal of genetic analysis in the 1990s, identification and cloning of resistance genes is now the goal. The first and until now the sole resistance gene which has been isolated and transformed into a susceptible apple cultivar is the gene HcrVf2 (Rvi6), responsible for the Vf scab resistance present in most classically bred scab resistant cultivars. Much effort is currently spent in the identification and positional cloning of other apple genes conferring resistance to apple scab and fire blight. In our labs, we identified the putative scab resistance gene Rvi15 and two fire blight resistance genes namely from ‘Evereste’ and Malus × robusta 5. The functionality of these candidate genes is currently under scrutiny by complementation experiments. However, the final goal is the creation of a product, e.g., an improved apple cultivar that is resistant to scab and fire blight. The ideal product would have advantages to the environment and producer, and should raise as little concern as possible with consumers. To accomplish this ‘ideal product’, we opted for the cisgenic approach by introducing the scab resistance gene HcrVf2 with its own regulatory sequences into the highly susceptible apple cultivar, ‘Gala’, through Agrobacterium transformation. All marker genes were eliminated after transformation. Similarly, we are currently introducing into both the readily developed cisgenic ‘Gala’ and in the untransformed ‘Gala’ the putative Malus own fire blight resistance gene candidates, aiming at both proof of functionality of the identified candidates and possibly at rapid development of a fire blight and scab resistant cisgenic apple.
Original languageEnglish
Title of host publicationII Genetically Modified Organisms in Horticulture Symposium
EditorsM.A. Veale
Place of PublicationWhite River, South Africa
PublisherISHS
Pages117-121
Volume974
ISBN (Print)9789066050976
DOIs
Publication statusPublished - 2013
EventII Genetically Modified Organisms in Horticulture Symposium -
Duration: 11 Sep 201115 Sep 2011

Conference

ConferenceII Genetically Modified Organisms in Horticulture Symposium
Period11/09/1115/09/11

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Erwinia amylovora
disease resistance
apples
genes
Venturia inaequalis
cultivars
Malus
marker-assisted selection
molecular cloning
Podosphaera leucotricha
breeds
regulatory sequences
Malus domestica
powdery mildew
Agrobacterium
genetic techniques and protocols
screening
genetic markers
seedlings
genotype

Cite this

Broggini, G. A. L., Durel, C. E., Vergne, E., Chevreau, E., Fahrentrapp, J., Vanblaere, T., ... Gessler, C. (2013). Cisgenic Approach for improved disease resistance in apple. In M. A. Veale (Ed.), II Genetically Modified Organisms in Horticulture Symposium (Vol. 974, pp. 117-121). White River, South Africa: ISHS. https://doi.org/10.17660/ActaHortic.2013.974.13
Broggini, G.A.L. ; Durel, C.E. ; Vergne, E. ; Chevreau, E. ; Fahrentrapp, J. ; Vanblaere, T. ; Peil, A. ; Flachowsky, H. ; Hanke, M.V. ; Krens, F.A. ; Schouten, H.J. ; Gessler, C. / Cisgenic Approach for improved disease resistance in apple. II Genetically Modified Organisms in Horticulture Symposium. editor / M.A. Veale. Vol. 974 White River, South Africa : ISHS, 2013. pp. 117-121
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title = "Cisgenic Approach for improved disease resistance in apple",
abstract = "Swiss and more generally European apple (Malus × domestica) production is hampered by several diseases, the most destructive being fire blight, caused by Erwinia amylovora. On the other hand, there are apple scab, caused by Venturia inaequalis and powdery mildew, caused by Podosphaera leucotricha, which represent the major phytosanitary problems. Classical breeding has produced many scab and mildew resistant cultivars and efforts to breed also fire blight resistant cultivars are currently undertaken. Marker assisted selection (MAS) increases efficiency by allowing early non-destructive screening of seedlings and identifying genotypes showing pyramids of resistance genes. If the development of markers for MAS was the primary goal of genetic analysis in the 1990s, identification and cloning of resistance genes is now the goal. The first and until now the sole resistance gene which has been isolated and transformed into a susceptible apple cultivar is the gene HcrVf2 (Rvi6), responsible for the Vf scab resistance present in most classically bred scab resistant cultivars. Much effort is currently spent in the identification and positional cloning of other apple genes conferring resistance to apple scab and fire blight. In our labs, we identified the putative scab resistance gene Rvi15 and two fire blight resistance genes namely from ‘Evereste’ and Malus × robusta 5. The functionality of these candidate genes is currently under scrutiny by complementation experiments. However, the final goal is the creation of a product, e.g., an improved apple cultivar that is resistant to scab and fire blight. The ideal product would have advantages to the environment and producer, and should raise as little concern as possible with consumers. To accomplish this ‘ideal product’, we opted for the cisgenic approach by introducing the scab resistance gene HcrVf2 with its own regulatory sequences into the highly susceptible apple cultivar, ‘Gala’, through Agrobacterium transformation. All marker genes were eliminated after transformation. Similarly, we are currently introducing into both the readily developed cisgenic ‘Gala’ and in the untransformed ‘Gala’ the putative Malus own fire blight resistance gene candidates, aiming at both proof of functionality of the identified candidates and possibly at rapid development of a fire blight and scab resistant cisgenic apple.",
author = "G.A.L. Broggini and C.E. Durel and E. Vergne and E. Chevreau and J. Fahrentrapp and T. Vanblaere and A. Peil and H. Flachowsky and M.V. Hanke and F.A. Krens and H.J. Schouten and C. Gessler",
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Broggini, GAL, Durel, CE, Vergne, E, Chevreau, E, Fahrentrapp, J, Vanblaere, T, Peil, A, Flachowsky, H, Hanke, MV, Krens, FA, Schouten, HJ & Gessler, C 2013, Cisgenic Approach for improved disease resistance in apple. in MA Veale (ed.), II Genetically Modified Organisms in Horticulture Symposium. vol. 974, ISHS, White River, South Africa, pp. 117-121, II Genetically Modified Organisms in Horticulture Symposium, 11/09/11. https://doi.org/10.17660/ActaHortic.2013.974.13

Cisgenic Approach for improved disease resistance in apple. / Broggini, G.A.L.; Durel, C.E.; Vergne, E.; Chevreau, E.; Fahrentrapp, J.; Vanblaere, T.; Peil, A.; Flachowsky, H.; Hanke, M.V.; Krens, F.A.; Schouten, H.J.; Gessler, C.

II Genetically Modified Organisms in Horticulture Symposium. ed. / M.A. Veale. Vol. 974 White River, South Africa : ISHS, 2013. p. 117-121.

Research output: Chapter in Book/Report/Conference proceedingConference paper

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T1 - Cisgenic Approach for improved disease resistance in apple

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AU - Peil, A.

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N2 - Swiss and more generally European apple (Malus × domestica) production is hampered by several diseases, the most destructive being fire blight, caused by Erwinia amylovora. On the other hand, there are apple scab, caused by Venturia inaequalis and powdery mildew, caused by Podosphaera leucotricha, which represent the major phytosanitary problems. Classical breeding has produced many scab and mildew resistant cultivars and efforts to breed also fire blight resistant cultivars are currently undertaken. Marker assisted selection (MAS) increases efficiency by allowing early non-destructive screening of seedlings and identifying genotypes showing pyramids of resistance genes. If the development of markers for MAS was the primary goal of genetic analysis in the 1990s, identification and cloning of resistance genes is now the goal. The first and until now the sole resistance gene which has been isolated and transformed into a susceptible apple cultivar is the gene HcrVf2 (Rvi6), responsible for the Vf scab resistance present in most classically bred scab resistant cultivars. Much effort is currently spent in the identification and positional cloning of other apple genes conferring resistance to apple scab and fire blight. In our labs, we identified the putative scab resistance gene Rvi15 and two fire blight resistance genes namely from ‘Evereste’ and Malus × robusta 5. The functionality of these candidate genes is currently under scrutiny by complementation experiments. However, the final goal is the creation of a product, e.g., an improved apple cultivar that is resistant to scab and fire blight. The ideal product would have advantages to the environment and producer, and should raise as little concern as possible with consumers. To accomplish this ‘ideal product’, we opted for the cisgenic approach by introducing the scab resistance gene HcrVf2 with its own regulatory sequences into the highly susceptible apple cultivar, ‘Gala’, through Agrobacterium transformation. All marker genes were eliminated after transformation. Similarly, we are currently introducing into both the readily developed cisgenic ‘Gala’ and in the untransformed ‘Gala’ the putative Malus own fire blight resistance gene candidates, aiming at both proof of functionality of the identified candidates and possibly at rapid development of a fire blight and scab resistant cisgenic apple.

AB - Swiss and more generally European apple (Malus × domestica) production is hampered by several diseases, the most destructive being fire blight, caused by Erwinia amylovora. On the other hand, there are apple scab, caused by Venturia inaequalis and powdery mildew, caused by Podosphaera leucotricha, which represent the major phytosanitary problems. Classical breeding has produced many scab and mildew resistant cultivars and efforts to breed also fire blight resistant cultivars are currently undertaken. Marker assisted selection (MAS) increases efficiency by allowing early non-destructive screening of seedlings and identifying genotypes showing pyramids of resistance genes. If the development of markers for MAS was the primary goal of genetic analysis in the 1990s, identification and cloning of resistance genes is now the goal. The first and until now the sole resistance gene which has been isolated and transformed into a susceptible apple cultivar is the gene HcrVf2 (Rvi6), responsible for the Vf scab resistance present in most classically bred scab resistant cultivars. Much effort is currently spent in the identification and positional cloning of other apple genes conferring resistance to apple scab and fire blight. In our labs, we identified the putative scab resistance gene Rvi15 and two fire blight resistance genes namely from ‘Evereste’ and Malus × robusta 5. The functionality of these candidate genes is currently under scrutiny by complementation experiments. However, the final goal is the creation of a product, e.g., an improved apple cultivar that is resistant to scab and fire blight. The ideal product would have advantages to the environment and producer, and should raise as little concern as possible with consumers. To accomplish this ‘ideal product’, we opted for the cisgenic approach by introducing the scab resistance gene HcrVf2 with its own regulatory sequences into the highly susceptible apple cultivar, ‘Gala’, through Agrobacterium transformation. All marker genes were eliminated after transformation. Similarly, we are currently introducing into both the readily developed cisgenic ‘Gala’ and in the untransformed ‘Gala’ the putative Malus own fire blight resistance gene candidates, aiming at both proof of functionality of the identified candidates and possibly at rapid development of a fire blight and scab resistant cisgenic apple.

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Broggini GAL, Durel CE, Vergne E, Chevreau E, Fahrentrapp J, Vanblaere T et al. Cisgenic Approach for improved disease resistance in apple. In Veale MA, editor, II Genetically Modified Organisms in Horticulture Symposium. Vol. 974. White River, South Africa: ISHS. 2013. p. 117-121 https://doi.org/10.17660/ActaHortic.2013.974.13