Circulation of Schmallenberg virus in Turkey, 2013

Sükrü Tonbak, Ahmet Kürsat Azkur, Züleyha Pestil, Emel Biyikli, Hasan Abayli, Ersoy Baydar, W.H.M. van der Poel, Hakan Bulut

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Schmallenberg virus (SBV) infection emerged in European domestic and wild ruminants in 2011. There is very limited information about the characterization of SBV isolates and the epidemiology of its infections in the rest of world, except for in European countries. We investigated the circulation of SBV in cattle herds in Central Anatolia, Turkey, in 2013. A total of 180 whole-blood samples were analyzed using real-time RT-PCR. The presence of SBV RNA was detected in 6 (3.3%) samples. For phylogenetic analysis and confirmation of real-time RT-PCR results, the S gene segment was amplified, sequenced, and compared to other segments. In addition, SBV-specific antibodies were detected in 87 (24.1%) of 360 sera using a virus neutralization test. In the S gene sequence analysis of four randomly selected samples, 98%-99% nucleotide identity was observed between our strains and SBVs isolated in European countries between 2011 and 2013. The results of this study indicate that SBV was in Turkey in 2013. Furthermore, the sequencing results suggest that it could be the same virus that is in European countries.

Original languageEnglish
Pages (from-to)175-180
JournalTurkish Journal of Veterinary and Animal Sciences
Volume40
Issue number2
DOIs
Publication statusPublished - 2016

Fingerprint

Turkey
Turkey (country)
Viruses
Real-Time Polymerase Chain Reaction
reverse transcriptase polymerase chain reaction
Neutralization Tests
RNA Viruses
Ruminants
neutralization tests
Virus Diseases
blood serum
sampling
infection
Genes
Sequence Analysis
Schmallenberg virus
epidemiology
ruminants
Epidemiology
Nucleotides

Keywords

  • Circulation
  • RT-PCR
  • Schmallenberg virus
  • Turkey

Cite this

Tonbak, S., Azkur, A. K., Pestil, Z., Biyikli, E., Abayli, H., Baydar, E., ... Bulut, H. (2016). Circulation of Schmallenberg virus in Turkey, 2013. Turkish Journal of Veterinary and Animal Sciences, 40(2), 175-180. https://doi.org/10.3906/vet-1507-3
Tonbak, Sükrü ; Azkur, Ahmet Kürsat ; Pestil, Züleyha ; Biyikli, Emel ; Abayli, Hasan ; Baydar, Ersoy ; van der Poel, W.H.M. ; Bulut, Hakan. / Circulation of Schmallenberg virus in Turkey, 2013. In: Turkish Journal of Veterinary and Animal Sciences. 2016 ; Vol. 40, No. 2. pp. 175-180.
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title = "Circulation of Schmallenberg virus in Turkey, 2013",
abstract = "Schmallenberg virus (SBV) infection emerged in European domestic and wild ruminants in 2011. There is very limited information about the characterization of SBV isolates and the epidemiology of its infections in the rest of world, except for in European countries. We investigated the circulation of SBV in cattle herds in Central Anatolia, Turkey, in 2013. A total of 180 whole-blood samples were analyzed using real-time RT-PCR. The presence of SBV RNA was detected in 6 (3.3{\%}) samples. For phylogenetic analysis and confirmation of real-time RT-PCR results, the S gene segment was amplified, sequenced, and compared to other segments. In addition, SBV-specific antibodies were detected in 87 (24.1{\%}) of 360 sera using a virus neutralization test. In the S gene sequence analysis of four randomly selected samples, 98{\%}-99{\%} nucleotide identity was observed between our strains and SBVs isolated in European countries between 2011 and 2013. The results of this study indicate that SBV was in Turkey in 2013. Furthermore, the sequencing results suggest that it could be the same virus that is in European countries.",
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Tonbak, S, Azkur, AK, Pestil, Z, Biyikli, E, Abayli, H, Baydar, E, van der Poel, WHM & Bulut, H 2016, 'Circulation of Schmallenberg virus in Turkey, 2013' Turkish Journal of Veterinary and Animal Sciences, vol. 40, no. 2, pp. 175-180. https://doi.org/10.3906/vet-1507-3

Circulation of Schmallenberg virus in Turkey, 2013. / Tonbak, Sükrü; Azkur, Ahmet Kürsat; Pestil, Züleyha; Biyikli, Emel; Abayli, Hasan; Baydar, Ersoy; van der Poel, W.H.M.; Bulut, Hakan.

In: Turkish Journal of Veterinary and Animal Sciences, Vol. 40, No. 2, 2016, p. 175-180.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Tonbak, Sükrü

AU - Azkur, Ahmet Kürsat

AU - Pestil, Züleyha

AU - Biyikli, Emel

AU - Abayli, Hasan

AU - Baydar, Ersoy

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AU - Bulut, Hakan

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N2 - Schmallenberg virus (SBV) infection emerged in European domestic and wild ruminants in 2011. There is very limited information about the characterization of SBV isolates and the epidemiology of its infections in the rest of world, except for in European countries. We investigated the circulation of SBV in cattle herds in Central Anatolia, Turkey, in 2013. A total of 180 whole-blood samples were analyzed using real-time RT-PCR. The presence of SBV RNA was detected in 6 (3.3%) samples. For phylogenetic analysis and confirmation of real-time RT-PCR results, the S gene segment was amplified, sequenced, and compared to other segments. In addition, SBV-specific antibodies were detected in 87 (24.1%) of 360 sera using a virus neutralization test. In the S gene sequence analysis of four randomly selected samples, 98%-99% nucleotide identity was observed between our strains and SBVs isolated in European countries between 2011 and 2013. The results of this study indicate that SBV was in Turkey in 2013. Furthermore, the sequencing results suggest that it could be the same virus that is in European countries.

AB - Schmallenberg virus (SBV) infection emerged in European domestic and wild ruminants in 2011. There is very limited information about the characterization of SBV isolates and the epidemiology of its infections in the rest of world, except for in European countries. We investigated the circulation of SBV in cattle herds in Central Anatolia, Turkey, in 2013. A total of 180 whole-blood samples were analyzed using real-time RT-PCR. The presence of SBV RNA was detected in 6 (3.3%) samples. For phylogenetic analysis and confirmation of real-time RT-PCR results, the S gene segment was amplified, sequenced, and compared to other segments. In addition, SBV-specific antibodies were detected in 87 (24.1%) of 360 sera using a virus neutralization test. In the S gene sequence analysis of four randomly selected samples, 98%-99% nucleotide identity was observed between our strains and SBVs isolated in European countries between 2011 and 2013. The results of this study indicate that SBV was in Turkey in 2013. Furthermore, the sequencing results suggest that it could be the same virus that is in European countries.

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Tonbak S, Azkur AK, Pestil Z, Biyikli E, Abayli H, Baydar E et al. Circulation of Schmallenberg virus in Turkey, 2013. Turkish Journal of Veterinary and Animal Sciences. 2016;40(2):175-180. https://doi.org/10.3906/vet-1507-3