Chronic simultaneous exposure of common carp (Cyprinus carpio) from embryonic to juvenile stage to drospirenone and gestodene at low ng/L level caused intersex

Pavel Šauer*, Jitka Tumová, Christoph Steinbach, Oksana Golovko, Hans Komen, Emmanuelle Maillot-Maréchal, Jana Máchová, Roman Grabic, Selim Aït-Aïssa, Hana Kocour Kroupová

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Synthetic progestins are emerging contaminants of the aquatic environment with endocrine disrupting potential. The main aim of the present study was to investigate the effects of the synthetic progestins gestodene, and drospirenone on sex differentiation in common carp (Cyprinus carpio) by histological analysis. To gain insights into the mechanisms behind the observations from the in vivo experiment on sex differentiation, we analyzed expression of genes involved in hypothalamus–pituitary–gonad (HPG) and hypothalamus–pituitary–thyroid (HPT) axes, histology of hepatopancreas, and in vitro bioassays. Carp were continuously exposed to concentrations of 2 ng/L of single progestins (gestodene or drospirenone) or to their mixture at concentration 2 ng/L of each. The exposure started 24 h after fertilization of eggs and concluded 160 days post-hatching. Our results showed that exposure of common carp to a binary mixture of drospirenone and gestodene caused increased incidence of intersex (32%) when compared to clean water and solvent control groups (both 3%). Intersex most probably was induced by a combination of multiple modes of action of the studied substances, namely anti-gonadotropic activity, interference with androgen receptor, and potentially also with HPT axis or estrogen receptor.

Original languageEnglish
Article number109912
JournalEcotoxicology and Environmental Safety
Volume188
DOIs
Publication statusPublished - 30 Jan 2020

Fingerprint

Carps
Progesterone Congeners
Sex Differentiation
Histology
Bioassay
Binary mixtures
Hepatopancreas
Androgen Receptors
Progestins
Genes
Fertilization
Estrogen Receptors
Biological Assay
Impurities
Eggs
Gene Expression
Control Groups
drospirenone
Gestodene
Water

Keywords

  • Adverse effects
  • Hormones
  • Pollutants
  • Progestogens
  • Reproduction

Cite this

Šauer, Pavel ; Tumová, Jitka ; Steinbach, Christoph ; Golovko, Oksana ; Komen, Hans ; Maillot-Maréchal, Emmanuelle ; Máchová, Jana ; Grabic, Roman ; Aït-Aïssa, Selim ; Kocour Kroupová, Hana. / Chronic simultaneous exposure of common carp (Cyprinus carpio) from embryonic to juvenile stage to drospirenone and gestodene at low ng/L level caused intersex. In: Ecotoxicology and Environmental Safety. 2020 ; Vol. 188.
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abstract = "Synthetic progestins are emerging contaminants of the aquatic environment with endocrine disrupting potential. The main aim of the present study was to investigate the effects of the synthetic progestins gestodene, and drospirenone on sex differentiation in common carp (Cyprinus carpio) by histological analysis. To gain insights into the mechanisms behind the observations from the in vivo experiment on sex differentiation, we analyzed expression of genes involved in hypothalamus–pituitary–gonad (HPG) and hypothalamus–pituitary–thyroid (HPT) axes, histology of hepatopancreas, and in vitro bioassays. Carp were continuously exposed to concentrations of 2 ng/L of single progestins (gestodene or drospirenone) or to their mixture at concentration 2 ng/L of each. The exposure started 24 h after fertilization of eggs and concluded 160 days post-hatching. Our results showed that exposure of common carp to a binary mixture of drospirenone and gestodene caused increased incidence of intersex (32{\%}) when compared to clean water and solvent control groups (both 3{\%}). Intersex most probably was induced by a combination of multiple modes of action of the studied substances, namely anti-gonadotropic activity, interference with androgen receptor, and potentially also with HPT axis or estrogen receptor.",
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Chronic simultaneous exposure of common carp (Cyprinus carpio) from embryonic to juvenile stage to drospirenone and gestodene at low ng/L level caused intersex. / Šauer, Pavel; Tumová, Jitka; Steinbach, Christoph; Golovko, Oksana; Komen, Hans; Maillot-Maréchal, Emmanuelle; Máchová, Jana; Grabic, Roman; Aït-Aïssa, Selim; Kocour Kroupová, Hana.

In: Ecotoxicology and Environmental Safety, Vol. 188, 109912, 30.01.2020.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Šauer, Pavel

AU - Tumová, Jitka

AU - Steinbach, Christoph

AU - Golovko, Oksana

AU - Komen, Hans

AU - Maillot-Maréchal, Emmanuelle

AU - Máchová, Jana

AU - Grabic, Roman

AU - Aït-Aïssa, Selim

AU - Kocour Kroupová, Hana

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KW - Hormones

KW - Pollutants

KW - Progestogens

KW - Reproduction

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