Chronic quercetin exposure affects fatty acid catabolism in rat lung

V.C.J. de Boer, E.M. van Schothorst, A.A. Dihal, H. van der Woude, I.C.W. Arts, I.M.C.M. Rietjens, P.C.H. Hollman, J. Keijer

Research output: Contribution to journalArticleAcademicpeer-review

48 Citations (Scopus)

Abstract

Dietary quercetin intake is suggested to be health promoting, but this assumption is mainly based on mechanistic studies performed in vitro. Previously, we identified rat lung as a quercetin target tissue. To assess relevant in vivo health effects of quercetin, we analyzed mechanisms of effect in rat lungs of a chronic (41 weeks) 1% quercetin diet using whole genome microarrays. We show here that fatty acid catabolism pathways, like beta-oxidation and ketogenesis, are up-regulated by Dietary quercetin intake is suggested to be health promoting, but this assumption is mainly based on mechanistic studies performed in vitro. Previously, we identified rat lung as a quercetin target tissue. To assess relevant in vivo health effects of quercetin, we analyzed mechanisms of effect in rat lungs of a chronic (41 weeks) 1% quercetin diet using whole genome microarrays. We show here that fatty acid catabolism pathways, like beta-oxidation and ketogenesis, are up-regulated by the long-term quercetin intervention. Up-regulation of genes (Hmgcs2, Ech1, Acox1, Pcca, Lpl and Acaa2) was verified and confirmed by quantitative real time PCR. In addition, free fatty acid levels were decreased in rats fed the quercetin diet, confirming that quercetin affects fatty acid catabolism. This in vivo study demonstrates for the first time that fatty acid catabolism is a relevant process that is affected in rats by chronic dietary quercetin
LanguageEnglish
Pages2847-2858
JournalCellular and Molecular Life Sciences
Volume63
Issue number23
DOIs
Publication statusPublished - 2006

Fingerprint

Quercetin
Fatty Acids
Lung
Diet
Health
Genome
Nonesterified Fatty Acids
Real-Time Polymerase Chain Reaction
Up-Regulation

Keywords

  • dependent anion channel
  • diet-induced obesity
  • colon-cancer cells
  • gene-expression
  • dna microarray
  • green tea
  • in-vitro
  • polyphenols
  • inhibition
  • mice

Cite this

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title = "Chronic quercetin exposure affects fatty acid catabolism in rat lung",
abstract = "Dietary quercetin intake is suggested to be health promoting, but this assumption is mainly based on mechanistic studies performed in vitro. Previously, we identified rat lung as a quercetin target tissue. To assess relevant in vivo health effects of quercetin, we analyzed mechanisms of effect in rat lungs of a chronic (41 weeks) 1{\%} quercetin diet using whole genome microarrays. We show here that fatty acid catabolism pathways, like beta-oxidation and ketogenesis, are up-regulated by Dietary quercetin intake is suggested to be health promoting, but this assumption is mainly based on mechanistic studies performed in vitro. Previously, we identified rat lung as a quercetin target tissue. To assess relevant in vivo health effects of quercetin, we analyzed mechanisms of effect in rat lungs of a chronic (41 weeks) 1{\%} quercetin diet using whole genome microarrays. We show here that fatty acid catabolism pathways, like beta-oxidation and ketogenesis, are up-regulated by the long-term quercetin intervention. Up-regulation of genes (Hmgcs2, Ech1, Acox1, Pcca, Lpl and Acaa2) was verified and confirmed by quantitative real time PCR. In addition, free fatty acid levels were decreased in rats fed the quercetin diet, confirming that quercetin affects fatty acid catabolism. This in vivo study demonstrates for the first time that fatty acid catabolism is a relevant process that is affected in rats by chronic dietary quercetin",
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author = "{de Boer}, V.C.J. and {van Schothorst}, E.M. and A.A. Dihal and {van der Woude}, H. and I.C.W. Arts and I.M.C.M. Rietjens and P.C.H. Hollman and J. Keijer",
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}

Chronic quercetin exposure affects fatty acid catabolism in rat lung. / de Boer, V.C.J.; van Schothorst, E.M.; Dihal, A.A.; van der Woude, H.; Arts, I.C.W.; Rietjens, I.M.C.M.; Hollman, P.C.H.; Keijer, J.

In: Cellular and Molecular Life Sciences, Vol. 63, No. 23, 2006, p. 2847-2858.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Chronic quercetin exposure affects fatty acid catabolism in rat lung

AU - de Boer, V.C.J.

AU - van Schothorst, E.M.

AU - Dihal, A.A.

AU - van der Woude, H.

AU - Arts, I.C.W.

AU - Rietjens, I.M.C.M.

AU - Hollman, P.C.H.

AU - Keijer, J.

PY - 2006

Y1 - 2006

N2 - Dietary quercetin intake is suggested to be health promoting, but this assumption is mainly based on mechanistic studies performed in vitro. Previously, we identified rat lung as a quercetin target tissue. To assess relevant in vivo health effects of quercetin, we analyzed mechanisms of effect in rat lungs of a chronic (41 weeks) 1% quercetin diet using whole genome microarrays. We show here that fatty acid catabolism pathways, like beta-oxidation and ketogenesis, are up-regulated by Dietary quercetin intake is suggested to be health promoting, but this assumption is mainly based on mechanistic studies performed in vitro. Previously, we identified rat lung as a quercetin target tissue. To assess relevant in vivo health effects of quercetin, we analyzed mechanisms of effect in rat lungs of a chronic (41 weeks) 1% quercetin diet using whole genome microarrays. We show here that fatty acid catabolism pathways, like beta-oxidation and ketogenesis, are up-regulated by the long-term quercetin intervention. Up-regulation of genes (Hmgcs2, Ech1, Acox1, Pcca, Lpl and Acaa2) was verified and confirmed by quantitative real time PCR. In addition, free fatty acid levels were decreased in rats fed the quercetin diet, confirming that quercetin affects fatty acid catabolism. This in vivo study demonstrates for the first time that fatty acid catabolism is a relevant process that is affected in rats by chronic dietary quercetin

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KW - dependent anion channel

KW - diet-induced obesity

KW - colon-cancer cells

KW - gene-expression

KW - dna microarray

KW - green tea

KW - in-vitro

KW - polyphenols

KW - inhibition

KW - mice

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DO - 10.1007/s00018-006-6316-z

M3 - Article

VL - 63

SP - 2847

EP - 2858

JO - Cellular and Molecular Life Sciences

T2 - Cellular and Molecular Life Sciences

JF - Cellular and Molecular Life Sciences

SN - 1420-682X

IS - 23

ER -