Chromatography: High-Performance Liquid Chromatography

H. Gika*, G. Kaklamanos, P. Manesiotis, G. Theodoridis

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

4 Citations (Scopus)


High-performance liquid chromatography (HPLC) is a major analytic tool in contemporary science, with possibly the highest number of systems installed and running globally. Modern HPLC offers high resolutions allowing the quantitative determination of target analytes within complex matrices by its compatibility with a number of detectors. The article describes the major technological characteristics of HPLC, reviewing separation mechanisms and their application in health and food science. Separation modes and media, key instrumental parameters, compatibility with detection modes, and applications are briefly discussed, aiming to provide helpful hints to the reader in the search for appropriate analytic techniques for a given task.

Original languageEnglish
Title of host publicationEncyclopedia of Food and Health
EditorsB. Caballero, P.M. Finglas, F. Toldrá
PublisherElsevier Inc. Academic Press
Number of pages7
ISBN (Electronic)9780123849533
ISBN (Print)9780123849472
Publication statusPublished - 14 Sep 2015


  • Affinity chromatography
  • Clinical analysis
  • Food analysis
  • High-performance liquid chromatography
  • HPLC
  • Ion exchange chromatography
  • Ion pair chromatography
  • LC-MS
  • Normal phase separations
  • Reverse phase separations
  • Superficially porous particles


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