Abstract
High-performance liquid chromatography (HPLC) is a major analytic tool in contemporary science, with possibly the highest number of systems installed and running globally. Modern HPLC offers high resolutions allowing the quantitative determination of target analytes within complex matrices by its compatibility with a number of detectors. The article describes the major technological characteristics of HPLC, reviewing separation mechanisms and their application in health and food science. Separation modes and media, key instrumental parameters, compatibility with detection modes, and applications are briefly discussed, aiming to provide helpful hints to the reader in the search for appropriate analytic techniques for a given task.
| Original language | English |
|---|---|
| Title of host publication | Encyclopedia of Food and Health |
| Editors | B. Caballero, P.M. Finglas, F. Toldrá |
| Publisher | Elsevier Inc. Academic Press |
| Pages | 93-99 |
| Number of pages | 7 |
| ISBN (Electronic) | 9780123849533 |
| ISBN (Print) | 9780123849472 |
| DOIs | |
| Publication status | Published - 14 Sep 2015 |
Keywords
- Affinity chromatography
- Clinical analysis
- Food analysis
- High-performance liquid chromatography
- HPLC
- Ion exchange chromatography
- Ion pair chromatography
- LC-MS
- Normal phase separations
- Reverse phase separations
- Superficially porous particles
- UHPLC