Chromatographic and Enzymatic Strategies To Reveal Differences between Amidated Pectins on a Molecular Level

S.E. Guillotin, N. Mey, E. Ananta, P. Boulenguer, H.A. Schols, A.G.J. Voragen

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

The intermolecular distributions of amide groups within two commercial LMA pectins was studied after removal of the methyl esters followed by fractionation of the different populations by anion exchange chromatography. The populations obtained had almost equal degrees of amidation while the values of the degree of blockiness were not the same, indicating also a different intramolecular distribution of the substituents considered as semirandom. Populations from the methyl-esterified amidated pectins showed a rather random distribution for almost all populations. A striking difference between these different populations was that, despite the same level of substitution, the ratio between amide groups and methyl esters varied significantly, indicating a heterogeneous amidation process.
Original languageEnglish
Pages (from-to)2032-2037
JournalBiomacromolecules
Volume7
Issue number6
DOIs
Publication statusPublished - 2006

Keywords

  • galacturonic acid distribution
  • plant-cell walls
  • substances
  • endopolygalacturonase
  • polysaccharide
  • behavior

Fingerprint Dive into the research topics of 'Chromatographic and Enzymatic Strategies To Reveal Differences between Amidated Pectins on a Molecular Level'. Together they form a unique fingerprint.

  • Cite this