Chlorophyll fluorescence imaging reveals genetic variation and loci for a photosynthetic trait in diploid potato

Aina E. Prinzenberg, Marcela Víquez-Zamora, Jeremy Harbinson, Pim Lindhout, Sjaak van Heusden*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Physiology and genetics are tightly interrelated. Understanding the genetic basis of a physiological trait such as the quantum yield of the photosystem II, or photosynthetic responses to environmental changes will benefit the understanding of these processes. By means of chlorophyll fluorescence (CF) imaging, the quantum yield of photosystem II can be determined rapidly, precisely and non-invasively. In this article, the genetic control and variation in the steady-state quantum yield of PSII (ΦPSII) is analyzed for diploid potato plants. Current progress in potato research and breeding is slow due to high levels of heterozygosity and complexity of tetraploid genetics. Diploid potatoes offer the possibility of overcoming this problem and advance research for one of the globally most important staple foods. With the help of a diploid genetic mapping population two genetic loci that were strongly associated with differences in ΦPSII were identified. This is a proof of principle that genetic analysis for ΦPSII can be done on potato. The effects of three different stress conditions that are important in potato cultivation were also tested: salt stress, low temperature and deficiency in the macronutrient phosphate. For the last two stresses, significant decreases in photosynthetic activity could be shown, revealing potential for stress detection with CF based tools. In general, our findings show the potential of high-throughput phenotyping for physiological research and breeding in potato.

Original languageEnglish
Pages (from-to)163-175
JournalPhysiologia Plantarum
Volume164
Issue number2
DOIs
Publication statusPublished - 1 Oct 2018

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Genetic Loci
Optical Imaging
Chlorophyll
Solanum tuberosum
Diploidy
diploidy
image analysis
potatoes
fluorescence
chlorophyll
genetic variation
loci
Photosystem II Protein Complex
photosystem II
Breeding
Research
Tetraploidy
staple foods
breeding
Population Genetics

Cite this

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title = "Chlorophyll fluorescence imaging reveals genetic variation and loci for a photosynthetic trait in diploid potato",
abstract = "Physiology and genetics are tightly interrelated. Understanding the genetic basis of a physiological trait such as the quantum yield of the photosystem II, or photosynthetic responses to environmental changes will benefit the understanding of these processes. By means of chlorophyll fluorescence (CF) imaging, the quantum yield of photosystem II can be determined rapidly, precisely and non-invasively. In this article, the genetic control and variation in the steady-state quantum yield of PSII (ΦPSII) is analyzed for diploid potato plants. Current progress in potato research and breeding is slow due to high levels of heterozygosity and complexity of tetraploid genetics. Diploid potatoes offer the possibility of overcoming this problem and advance research for one of the globally most important staple foods. With the help of a diploid genetic mapping population two genetic loci that were strongly associated with differences in ΦPSII were identified. This is a proof of principle that genetic analysis for ΦPSII can be done on potato. The effects of three different stress conditions that are important in potato cultivation were also tested: salt stress, low temperature and deficiency in the macronutrient phosphate. For the last two stresses, significant decreases in photosynthetic activity could be shown, revealing potential for stress detection with CF based tools. In general, our findings show the potential of high-throughput phenotyping for physiological research and breeding in potato.",
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Chlorophyll fluorescence imaging reveals genetic variation and loci for a photosynthetic trait in diploid potato. / Prinzenberg, Aina E.; Víquez-Zamora, Marcela; Harbinson, Jeremy; Lindhout, Pim; van Heusden, Sjaak.

In: Physiologia Plantarum, Vol. 164, No. 2, 01.10.2018, p. 163-175.

Research output: Contribution to journalArticleAcademicpeer-review

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T1 - Chlorophyll fluorescence imaging reveals genetic variation and loci for a photosynthetic trait in diploid potato

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AU - Víquez-Zamora, Marcela

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AU - Lindhout, Pim

AU - van Heusden, Sjaak

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