Chitosan as coagulant on cyanobacteria in lake restoration management may cause rapid cell lysis

Maira Nunes Teixeira Mucci*, Natalia Pessoa Noyma, Leonardo de Magalhães, Marcela Miranda, Frank van Oosterhout, Iamê Alves Guedes, Vera L.M. Huszar, Marcelo Manzi Marinho, Miquel Lürling

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

25 Citations (Scopus)

Abstract

Combining coagulant and ballast to remove cyanobacteria from the water column is a promising restoration technique to mitigate cyanobacterial nuisance in surface waters. The organic, biodegradable polymer chitosan has been promoted as a coagulant and is viewed as non-toxic. In this study, we show that chitosan may rapidly compromise membrane integrity and kill certain cyanobacteria leading to release of cell contents in the water. A strain of Cylindrospermopsis raciborskii and one strain of Planktothrix agardhii were most sensitive. A 1.3 h exposure to a low dose of 0.5 mg l−1 chitosan already almost completely killed these cultures resulting in release of cell contents. After 24 h, reductions in PSII efficiencies of all cyanobacteria tested were observed. EC50 values varied from around 0.5 mg l−1 chitosan for the two sensitive strains, via about 5 mg l−1 chitosan for an Aphanizomenon flos-aquae strain, a toxic P. agardhii strain and two Anabaena cylindrica cultures, to more than 8 mg l−1 chitosan for a Microcystis aeruginosa strain and another A. flos-aquae strain. Differences in sensitivity to chitosan might be related to polymeric substances that surround cyanobacteria. Rapid lysis of toxic strains is likely and when chitosan flocking and sinking of cyanobacteria is considered in lake restoration, flocculation efficacy studies should be complemented with investigation on the effects of chitosan on the cyanobacteria assemblage being targeted.

Original languageEnglish
Pages (from-to)121-130
JournalWater Research
Volume118
DOIs
Publication statusPublished - 2017

Keywords

  • Cell lysis
  • Cell viability
  • Cyanobacterial blooms
  • Eutrophication
  • Lake restoration
  • Photosystem II efficiency

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