Chicken immune response after in ovo Immunization with Chimeric TLR5 activating flagellin of campylobacter jejuni

Katarzyna A. Radomska, Mahdi M. Vaezirad, Koen M. Verstappen, Marc M.S.M. Wösten, Jaap A. Wagenaar, Jos P.M. van Putten

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Campylobacter jejuni is the main cause of bacterial food-borne diseases in developed countries. Chickens are the most important source of human infection. Vaccination of poultry is an attractive strategy to reduce the number of C. jejuni in the intestinal tract of chickens. We investigated the immunogenicity and protective efficacy of a recombinant C. jejuni flagellin-based subunit vaccine with intrinsic adjuvant activity. Toll-like receptor activation assays demonstrated the purity and TLR5 stimulating (adjuvant) activity of the vaccine. The antigen (20-40 μg) was administered in ovo to 18 day-old chicken embryos. Serum samples and intestinal content were assessed for antigen-specific systemic and mucosal humoral immune responses. In ovo vaccination resulted in the successful generation of IgY and IgM serum antibodies against the flagellin-based subunit vaccine as determined by ELISA and Western blotting. Vaccination did not induce significant amounts of flagellin-specific secretory IgA in the chicken intestine. Challenge of chickens with C. jejuni yielded similar intestinal colonization levels for vaccinated and control animals. Our results indicate that in ovo delivery of recombinant C. jejuni flagellin subunit vaccine is a feasible approach to yield a systemic humoral immune response in chickens but that a mucosal immune response may be needed to reduce C. jejuni colonization.

Original languageEnglish
Article numbere0164837
JournalPLoS ONE
Volume11
Issue number10
DOIs
Publication statusPublished - 2016

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Immunization
Flagellin
flagellin
Campylobacter jejuni
Subunit Vaccines
Chickens
immunization
immune response
chickens
subunit vaccines
Mucosal Immunity
Vaccination
vaccination
Humoral Immunity
Antigens
humoral immunity
Secretory Immunoglobulin A
Poultry
adjuvants
Toll-Like Receptors

Cite this

Radomska, K. A., Vaezirad, M. M., Verstappen, K. M., Wösten, M. M. S. M., Wagenaar, J. A., & van Putten, J. P. M. (2016). Chicken immune response after in ovo Immunization with Chimeric TLR5 activating flagellin of campylobacter jejuni. PLoS ONE, 11(10), [e0164837]. https://doi.org/10.1371/journal.pone.0164837
Radomska, Katarzyna A. ; Vaezirad, Mahdi M. ; Verstappen, Koen M. ; Wösten, Marc M.S.M. ; Wagenaar, Jaap A. ; van Putten, Jos P.M. / Chicken immune response after in ovo Immunization with Chimeric TLR5 activating flagellin of campylobacter jejuni. In: PLoS ONE. 2016 ; Vol. 11, No. 10.
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abstract = "Campylobacter jejuni is the main cause of bacterial food-borne diseases in developed countries. Chickens are the most important source of human infection. Vaccination of poultry is an attractive strategy to reduce the number of C. jejuni in the intestinal tract of chickens. We investigated the immunogenicity and protective efficacy of a recombinant C. jejuni flagellin-based subunit vaccine with intrinsic adjuvant activity. Toll-like receptor activation assays demonstrated the purity and TLR5 stimulating (adjuvant) activity of the vaccine. The antigen (20-40 μg) was administered in ovo to 18 day-old chicken embryos. Serum samples and intestinal content were assessed for antigen-specific systemic and mucosal humoral immune responses. In ovo vaccination resulted in the successful generation of IgY and IgM serum antibodies against the flagellin-based subunit vaccine as determined by ELISA and Western blotting. Vaccination did not induce significant amounts of flagellin-specific secretory IgA in the chicken intestine. Challenge of chickens with C. jejuni yielded similar intestinal colonization levels for vaccinated and control animals. Our results indicate that in ovo delivery of recombinant C. jejuni flagellin subunit vaccine is a feasible approach to yield a systemic humoral immune response in chickens but that a mucosal immune response may be needed to reduce C. jejuni colonization.",
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Radomska, KA, Vaezirad, MM, Verstappen, KM, Wösten, MMSM, Wagenaar, JA & van Putten, JPM 2016, 'Chicken immune response after in ovo Immunization with Chimeric TLR5 activating flagellin of campylobacter jejuni' PLoS ONE, vol. 11, no. 10, e0164837. https://doi.org/10.1371/journal.pone.0164837

Chicken immune response after in ovo Immunization with Chimeric TLR5 activating flagellin of campylobacter jejuni. / Radomska, Katarzyna A.; Vaezirad, Mahdi M.; Verstappen, Koen M.; Wösten, Marc M.S.M.; Wagenaar, Jaap A.; van Putten, Jos P.M.

In: PLoS ONE, Vol. 11, No. 10, e0164837, 2016.

Research output: Contribution to journalArticleAcademicpeer-review

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AB - Campylobacter jejuni is the main cause of bacterial food-borne diseases in developed countries. Chickens are the most important source of human infection. Vaccination of poultry is an attractive strategy to reduce the number of C. jejuni in the intestinal tract of chickens. We investigated the immunogenicity and protective efficacy of a recombinant C. jejuni flagellin-based subunit vaccine with intrinsic adjuvant activity. Toll-like receptor activation assays demonstrated the purity and TLR5 stimulating (adjuvant) activity of the vaccine. The antigen (20-40 μg) was administered in ovo to 18 day-old chicken embryos. Serum samples and intestinal content were assessed for antigen-specific systemic and mucosal humoral immune responses. In ovo vaccination resulted in the successful generation of IgY and IgM serum antibodies against the flagellin-based subunit vaccine as determined by ELISA and Western blotting. Vaccination did not induce significant amounts of flagellin-specific secretory IgA in the chicken intestine. Challenge of chickens with C. jejuni yielded similar intestinal colonization levels for vaccinated and control animals. Our results indicate that in ovo delivery of recombinant C. jejuni flagellin subunit vaccine is a feasible approach to yield a systemic humoral immune response in chickens but that a mucosal immune response may be needed to reduce C. jejuni colonization.

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Radomska KA, Vaezirad MM, Verstappen KM, Wösten MMSM, Wagenaar JA, van Putten JPM. Chicken immune response after in ovo Immunization with Chimeric TLR5 activating flagellin of campylobacter jejuni. PLoS ONE. 2016;11(10). e0164837. https://doi.org/10.1371/journal.pone.0164837