Chemotaxis and oospore formation in Phytophthora sojae are controlled by G-protein-coupled receptors with a phosphatidylinositol phosphate kinase domain

X. Yang, W. Zhao, C. Hua, X. Zheng, M. Jing, D. Li, F. Govers, H.J.G. Meijer, Y. Wang

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)

Abstract

G-protein-coupled receptors (GPCRs) are key cellular components that mediate extracellular signals into intracellular responses. Genome mining revealed that Phytophthora spp. have over 60 GPCR genes among which a prominent class of 12 encoding novel proteins with an N-terminal GPCR domain fused to a C-terminal phosphatidylinositol phosphate kinase (PIPK) domain. This study focuses on two GPCR-PIPKs (GKs) in Phytophthora sojae. PsGK4 and PsGK5 are differentially expressed during the life cycle with the highest expression in cysts and during cyst germination, and at late infection stages. In P.¿sojae transformants that constitutively express RFP-tagged PsGK4 and PsGK5, the fusion proteins in hyphae reside in small, rapidly moving vesicular-like structures. Functional analysis using gene silencing showed that PsGK4-silenced transformants displayed higher levels of encystment and a reduced cyst germination rate when compared with the recipient strain. Moreover, GK4 deficiency (or reduction) resulted in severe defects in zoospore chemotaxis towards isoflavones and soybean roots. In contrast, PsGK5-silenced transformants exhibited no obvious defects in asexual development but oospore production was severely impaired. Both, PsGK4- and PsGK5-silenced transformants showed reduced pathogenicity. These results point to involvement of GKs in zoospore behaviour, chemotaxis and oospore development, and suggest that PsGK4 and PsGK5 each head independent signalling pathways.
Original languageEnglish
Pages (from-to)382-394
Number of pages13
JournalMolecular Microbiology
Volume88
Issue number2
DOIs
Publication statusPublished - 2013

Keywords

  • sexual reproduction
  • gene-expression
  • mating hormone
  • plasma-membrane
  • alpha-subunit
  • infestans
  • pathogen
  • oomycete
  • isoflavones
  • identification

Fingerprint

Dive into the research topics of 'Chemotaxis and oospore formation in Phytophthora sojae are controlled by G-protein-coupled receptors with a phosphatidylinositol phosphate kinase domain'. Together they form a unique fingerprint.

Cite this