Abstract
Both in mammals and in fish, CXC chemokines activate leukocytes and regulate their migration both under normal physiological and inflammatory conditions. Moreover, in mammalian neutrophils CXC chemokines also stimulate the formation of neutrophil extracellular traps (NETs). Here, we investigated the effects of recombinant carp CXCL8s and CXCb1 on NET formation in neutrophils from the head (HK) and trunk (TK) kidney of carp. We found that neither recombinant CXCL8s nor CXCb1 stimulated DNA release in HK-derived neutrophils, while in TK-derived cells rcCXCb1 stimulated the release of NETs, composed of extracellular DNA co-localized with citrulline H3 histone and neutrophil elastase. Furthermore, CXCb1-induced NET release required NADPH oxidase activity, while it did not change upon treatment with CXCR inhibitors. In conclusion, we demonstrated, for the first time in fish, that CXCb1 chemokine induces formation of NETs in TK-derived neutrophils and this process is ROS-dependent. The difference between HK and TK-derived neutrophils is probably related to differences in the maturation state of these cells.
| Original language | English |
|---|---|
| Article number | 103521 |
| Journal | Developmental and Comparative Immunology |
| Volume | 103 |
| DOIs | |
| Publication status | Published - 1 Feb 2020 |
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Keywords
- CXC chemokines
- Histone H3
- NADPH oxidase
- Neutrophil elastase
- Neutrophil extracellular traps
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Chemokine CXCb1 stimulates formation of NETs in trunk kidney neutrophils of common carp. / Pijanowski, Lukasz; Verburg van Kemenade, Lidy; Chadzinska, Magdalena.
In: Developmental and Comparative Immunology, Vol. 103, 103521, 01.02.2020.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Chemokine CXCb1 stimulates formation of NETs in trunk kidney neutrophils of common carp
AU - Pijanowski, Lukasz
AU - Verburg van Kemenade, Lidy
AU - Chadzinska, Magdalena
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Both in mammals and in fish, CXC chemokines activate leukocytes and regulate their migration both under normal physiological and inflammatory conditions. Moreover, in mammalian neutrophils CXC chemokines also stimulate the formation of neutrophil extracellular traps (NETs). Here, we investigated the effects of recombinant carp CXCL8s and CXCb1 on NET formation in neutrophils from the head (HK) and trunk (TK) kidney of carp. We found that neither recombinant CXCL8s nor CXCb1 stimulated DNA release in HK-derived neutrophils, while in TK-derived cells rcCXCb1 stimulated the release of NETs, composed of extracellular DNA co-localized with citrulline H3 histone and neutrophil elastase. Furthermore, CXCb1-induced NET release required NADPH oxidase activity, while it did not change upon treatment with CXCR inhibitors. In conclusion, we demonstrated, for the first time in fish, that CXCb1 chemokine induces formation of NETs in TK-derived neutrophils and this process is ROS-dependent. The difference between HK and TK-derived neutrophils is probably related to differences in the maturation state of these cells.
AB - Both in mammals and in fish, CXC chemokines activate leukocytes and regulate their migration both under normal physiological and inflammatory conditions. Moreover, in mammalian neutrophils CXC chemokines also stimulate the formation of neutrophil extracellular traps (NETs). Here, we investigated the effects of recombinant carp CXCL8s and CXCb1 on NET formation in neutrophils from the head (HK) and trunk (TK) kidney of carp. We found that neither recombinant CXCL8s nor CXCb1 stimulated DNA release in HK-derived neutrophils, while in TK-derived cells rcCXCb1 stimulated the release of NETs, composed of extracellular DNA co-localized with citrulline H3 histone and neutrophil elastase. Furthermore, CXCb1-induced NET release required NADPH oxidase activity, while it did not change upon treatment with CXCR inhibitors. In conclusion, we demonstrated, for the first time in fish, that CXCb1 chemokine induces formation of NETs in TK-derived neutrophils and this process is ROS-dependent. The difference between HK and TK-derived neutrophils is probably related to differences in the maturation state of these cells.
KW - CXC chemokines
KW - Histone H3
KW - NADPH oxidase
KW - Neutrophil elastase
KW - Neutrophil extracellular traps
U2 - 10.1016/j.dci.2019.103521
DO - 10.1016/j.dci.2019.103521
M3 - Article
VL - 103
JO - Developmental and Comparative Immunology
JF - Developmental and Comparative Immunology
SN - 0145-305X
M1 - 103521
ER -