Chemokine CXCb1 stimulates formation of NETs in trunk kidney neutrophils of common carp

Lukasz Pijanowski, Lidy Verburg van Kemenade, Magdalena Chadzinska*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

Both in mammals and in fish, CXC chemokines activate leukocytes and regulate their migration both under normal physiological and inflammatory conditions. Moreover, in mammalian neutrophils CXC chemokines also stimulate the formation of neutrophil extracellular traps (NETs). Here, we investigated the effects of recombinant carp CXCL8s and CXCb1 on NET formation in neutrophils from the head (HK) and trunk (TK) kidney of carp. We found that neither recombinant CXCL8s nor CXCb1 stimulated DNA release in HK-derived neutrophils, while in TK-derived cells rcCXCb1 stimulated the release of NETs, composed of extracellular DNA co-localized with citrulline H3 histone and neutrophil elastase. Furthermore, CXCb1-induced NET release required NADPH oxidase activity, while it did not change upon treatment with CXCR inhibitors. In conclusion, we demonstrated, for the first time in fish, that CXCb1 chemokine induces formation of NETs in TK-derived neutrophils and this process is ROS-dependent. The difference between HK and TK-derived neutrophils is probably related to differences in the maturation state of these cells.

Original languageEnglish
Article number103521
JournalDevelopmental and Comparative Immunology
Volume103
DOIs
Publication statusPublished - 1 Feb 2020

Fingerprint

Carps
Chemokines
Neutrophils
Kidney
CXC Chemokines
Fishes
Citrulline
Leukocyte Elastase
NADPH Oxidase
DNA
Histones
Mammals
Leukocytes
Head
Extracellular Traps

Keywords

  • CXC chemokines
  • Histone H3
  • NADPH oxidase
  • Neutrophil elastase
  • Neutrophil extracellular traps

Cite this

Pijanowski, Lukasz ; Verburg van Kemenade, Lidy ; Chadzinska, Magdalena. / Chemokine CXCb1 stimulates formation of NETs in trunk kidney neutrophils of common carp. In: Developmental and Comparative Immunology. 2020 ; Vol. 103.
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title = "Chemokine CXCb1 stimulates formation of NETs in trunk kidney neutrophils of common carp",
abstract = "Both in mammals and in fish, CXC chemokines activate leukocytes and regulate their migration both under normal physiological and inflammatory conditions. Moreover, in mammalian neutrophils CXC chemokines also stimulate the formation of neutrophil extracellular traps (NETs). Here, we investigated the effects of recombinant carp CXCL8s and CXCb1 on NET formation in neutrophils from the head (HK) and trunk (TK) kidney of carp. We found that neither recombinant CXCL8s nor CXCb1 stimulated DNA release in HK-derived neutrophils, while in TK-derived cells rcCXCb1 stimulated the release of NETs, composed of extracellular DNA co-localized with citrulline H3 histone and neutrophil elastase. Furthermore, CXCb1-induced NET release required NADPH oxidase activity, while it did not change upon treatment with CXCR inhibitors. In conclusion, we demonstrated, for the first time in fish, that CXCb1 chemokine induces formation of NETs in TK-derived neutrophils and this process is ROS-dependent. The difference between HK and TK-derived neutrophils is probably related to differences in the maturation state of these cells.",
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author = "Lukasz Pijanowski and {Verburg van Kemenade}, Lidy and Magdalena Chadzinska",
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Chemokine CXCb1 stimulates formation of NETs in trunk kidney neutrophils of common carp. / Pijanowski, Lukasz; Verburg van Kemenade, Lidy; Chadzinska, Magdalena.

In: Developmental and Comparative Immunology, Vol. 103, 103521, 01.02.2020.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Chemokine CXCb1 stimulates formation of NETs in trunk kidney neutrophils of common carp

AU - Pijanowski, Lukasz

AU - Verburg van Kemenade, Lidy

AU - Chadzinska, Magdalena

PY - 2020/2/1

Y1 - 2020/2/1

N2 - Both in mammals and in fish, CXC chemokines activate leukocytes and regulate their migration both under normal physiological and inflammatory conditions. Moreover, in mammalian neutrophils CXC chemokines also stimulate the formation of neutrophil extracellular traps (NETs). Here, we investigated the effects of recombinant carp CXCL8s and CXCb1 on NET formation in neutrophils from the head (HK) and trunk (TK) kidney of carp. We found that neither recombinant CXCL8s nor CXCb1 stimulated DNA release in HK-derived neutrophils, while in TK-derived cells rcCXCb1 stimulated the release of NETs, composed of extracellular DNA co-localized with citrulline H3 histone and neutrophil elastase. Furthermore, CXCb1-induced NET release required NADPH oxidase activity, while it did not change upon treatment with CXCR inhibitors. In conclusion, we demonstrated, for the first time in fish, that CXCb1 chemokine induces formation of NETs in TK-derived neutrophils and this process is ROS-dependent. The difference between HK and TK-derived neutrophils is probably related to differences in the maturation state of these cells.

AB - Both in mammals and in fish, CXC chemokines activate leukocytes and regulate their migration both under normal physiological and inflammatory conditions. Moreover, in mammalian neutrophils CXC chemokines also stimulate the formation of neutrophil extracellular traps (NETs). Here, we investigated the effects of recombinant carp CXCL8s and CXCb1 on NET formation in neutrophils from the head (HK) and trunk (TK) kidney of carp. We found that neither recombinant CXCL8s nor CXCb1 stimulated DNA release in HK-derived neutrophils, while in TK-derived cells rcCXCb1 stimulated the release of NETs, composed of extracellular DNA co-localized with citrulline H3 histone and neutrophil elastase. Furthermore, CXCb1-induced NET release required NADPH oxidase activity, while it did not change upon treatment with CXCR inhibitors. In conclusion, we demonstrated, for the first time in fish, that CXCb1 chemokine induces formation of NETs in TK-derived neutrophils and this process is ROS-dependent. The difference between HK and TK-derived neutrophils is probably related to differences in the maturation state of these cells.

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