Chemoenzymatic synthesis of biotin-appended analogues of gangliosides GM2, GM1, GD1 and GalNAc-GD1a for solid-phase applications and improved ELISA tests

A. Pukin, D.E.A. Florack, D. Brochu, B. van Lagen, G.M. Visser, T. Wennekes, M. Gilbert, H. Zuilhof

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Biotinylated analogues of gangliosides GM2, GM1, GD1a and GalNAc-GD1a were synthesized in high yields using glycosyltransferases from Campylobacter jejuni. The presence of a biotin moiety in the aglycone part of these mimics allows for attachment of these materials onto various streptavidin-coated surfaces. Analysis of the interaction of biotin-appended GM1 with the B subunit of Escherichia coli heat-labile enterotoxin performed in a modified ELISA procedure shows the potential of this compound to replace the natural GM1 in toxin detection.
Original languageEnglish
Pages (from-to)5809-5815
JournalOrganic & Biomolecular Chemistry
Volume9
Issue number16
DOIs
Publication statusPublished - 2011

Keywords

  • surface-plasmon resonance
  • carbohydrate microarrays
  • campylobacter-jejuni
  • cholera-toxin
  • oligosaccharide microarrays
  • biological-systems
  • guillain-barre
  • nmr analysis
  • binding
  • sugar

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