Chemical investigation of Pinguicula lusitanica by HPLC-MS and HPLC-SPE-NMR

T. Grevenstuk, D.H.J.J.J. Van, J.J.M. Vervoort, A. Romano

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Eight compounds from the methanolic extract of in vitro grown Pinguicula lusitanica (L.), including four iridoid and two phenylethanoid glycosides, were isolated and tentatively assigned. P. lusitanica is an insectivorous plant that grows wild along the coastal area of Western Europe, from Scotland to Iberia, and Morocco in north-western Africa [1]. Wild populations of P. lusitanica are becoming increasingly scarce emphasizing the importance of in vitro cultures, which provide a fast mean of obtaining large quantities of plant material without jeopardizing the vulnerable wild populations. Previous analysis by GC-MS reported the presence of compounds with biological activity in the volatile fraction of the methanolic extract. The extract was prepared by grinding in vitro grown P. lusitanica shoots and extracting with methanol while being continuously stirred. The extract was filtered, concentrated in a rotary-evaporator under reduced pressure, dissolved in water and freeze dried. The crude extract was cleaned from apolar compounds using a SPE column before being analyzed by HPLC-MS/SPE-NMR. All major peaks of the HPLC chromatogram were trapped with SPE cartridges and subsequently analyzed by 1H-NMR. This study describes the chemical investigation of the insectivorous plant species P. lusitanica by HPLC-MS/SPE-NMR which made it possible to obtain a chemical profile in short time in comparison to traditional preparative chromatography methods [2].
Original languageEnglish
Title of host publicationProceedings of the 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF, Athens, Greece, 3-8 August 2008
Pages1101
Volume74
DOIs
Publication statusPublished - 2008
Event7th Joint Meeting of GA, AFERP, ASP, PSE & SIF, Athens, Greece -
Duration: 3 Aug 20088 Aug 2008

Conference

Conference7th Joint Meeting of GA, AFERP, ASP, PSE & SIF, Athens, Greece
Period3/08/088/08/08

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Pinguicula
high performance liquid chromatography
extracts
evaporators
wild plants
Northern Africa
grinding
Western European region
Morocco
Iberian Peninsula
Western Africa
in vitro culture
Scotland
glycosides
bioactive properties
chromatography
methanol
shoots
water

Cite this

Grevenstuk, T., Van, D. H. J. J. J., Vervoort, J. J. M., & Romano, A. (2008). Chemical investigation of Pinguicula lusitanica by HPLC-MS and HPLC-SPE-NMR. In Proceedings of the 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF, Athens, Greece, 3-8 August 2008 (Vol. 74, pp. 1101) https://doi.org/10.1055/s-0028-1084581
Grevenstuk, T. ; Van, D.H.J.J.J. ; Vervoort, J.J.M. ; Romano, A. / Chemical investigation of Pinguicula lusitanica by HPLC-MS and HPLC-SPE-NMR. Proceedings of the 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF, Athens, Greece, 3-8 August 2008. Vol. 74 2008. pp. 1101
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abstract = "Eight compounds from the methanolic extract of in vitro grown Pinguicula lusitanica (L.), including four iridoid and two phenylethanoid glycosides, were isolated and tentatively assigned. P. lusitanica is an insectivorous plant that grows wild along the coastal area of Western Europe, from Scotland to Iberia, and Morocco in north-western Africa [1]. Wild populations of P. lusitanica are becoming increasingly scarce emphasizing the importance of in vitro cultures, which provide a fast mean of obtaining large quantities of plant material without jeopardizing the vulnerable wild populations. Previous analysis by GC-MS reported the presence of compounds with biological activity in the volatile fraction of the methanolic extract. The extract was prepared by grinding in vitro grown P. lusitanica shoots and extracting with methanol while being continuously stirred. The extract was filtered, concentrated in a rotary-evaporator under reduced pressure, dissolved in water and freeze dried. The crude extract was cleaned from apolar compounds using a SPE column before being analyzed by HPLC-MS/SPE-NMR. All major peaks of the HPLC chromatogram were trapped with SPE cartridges and subsequently analyzed by 1H-NMR. This study describes the chemical investigation of the insectivorous plant species P. lusitanica by HPLC-MS/SPE-NMR which made it possible to obtain a chemical profile in short time in comparison to traditional preparative chromatography methods [2].",
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Grevenstuk, T, Van, DHJJJ, Vervoort, JJM & Romano, A 2008, Chemical investigation of Pinguicula lusitanica by HPLC-MS and HPLC-SPE-NMR. in Proceedings of the 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF, Athens, Greece, 3-8 August 2008. vol. 74, pp. 1101, 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF, Athens, Greece, 3/08/08. https://doi.org/10.1055/s-0028-1084581

Chemical investigation of Pinguicula lusitanica by HPLC-MS and HPLC-SPE-NMR. / Grevenstuk, T.; Van, D.H.J.J.J.; Vervoort, J.J.M.; Romano, A.

Proceedings of the 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF, Athens, Greece, 3-8 August 2008. Vol. 74 2008. p. 1101.

Research output: Chapter in Book/Report/Conference proceedingAbstract

TY - CHAP

T1 - Chemical investigation of Pinguicula lusitanica by HPLC-MS and HPLC-SPE-NMR

AU - Grevenstuk, T.

AU - Van, D.H.J.J.J.

AU - Vervoort, J.J.M.

AU - Romano, A.

PY - 2008

Y1 - 2008

N2 - Eight compounds from the methanolic extract of in vitro grown Pinguicula lusitanica (L.), including four iridoid and two phenylethanoid glycosides, were isolated and tentatively assigned. P. lusitanica is an insectivorous plant that grows wild along the coastal area of Western Europe, from Scotland to Iberia, and Morocco in north-western Africa [1]. Wild populations of P. lusitanica are becoming increasingly scarce emphasizing the importance of in vitro cultures, which provide a fast mean of obtaining large quantities of plant material without jeopardizing the vulnerable wild populations. Previous analysis by GC-MS reported the presence of compounds with biological activity in the volatile fraction of the methanolic extract. The extract was prepared by grinding in vitro grown P. lusitanica shoots and extracting with methanol while being continuously stirred. The extract was filtered, concentrated in a rotary-evaporator under reduced pressure, dissolved in water and freeze dried. The crude extract was cleaned from apolar compounds using a SPE column before being analyzed by HPLC-MS/SPE-NMR. All major peaks of the HPLC chromatogram were trapped with SPE cartridges and subsequently analyzed by 1H-NMR. This study describes the chemical investigation of the insectivorous plant species P. lusitanica by HPLC-MS/SPE-NMR which made it possible to obtain a chemical profile in short time in comparison to traditional preparative chromatography methods [2].

AB - Eight compounds from the methanolic extract of in vitro grown Pinguicula lusitanica (L.), including four iridoid and two phenylethanoid glycosides, were isolated and tentatively assigned. P. lusitanica is an insectivorous plant that grows wild along the coastal area of Western Europe, from Scotland to Iberia, and Morocco in north-western Africa [1]. Wild populations of P. lusitanica are becoming increasingly scarce emphasizing the importance of in vitro cultures, which provide a fast mean of obtaining large quantities of plant material without jeopardizing the vulnerable wild populations. Previous analysis by GC-MS reported the presence of compounds with biological activity in the volatile fraction of the methanolic extract. The extract was prepared by grinding in vitro grown P. lusitanica shoots and extracting with methanol while being continuously stirred. The extract was filtered, concentrated in a rotary-evaporator under reduced pressure, dissolved in water and freeze dried. The crude extract was cleaned from apolar compounds using a SPE column before being analyzed by HPLC-MS/SPE-NMR. All major peaks of the HPLC chromatogram were trapped with SPE cartridges and subsequently analyzed by 1H-NMR. This study describes the chemical investigation of the insectivorous plant species P. lusitanica by HPLC-MS/SPE-NMR which made it possible to obtain a chemical profile in short time in comparison to traditional preparative chromatography methods [2].

U2 - 10.1055/s-0028-1084581

DO - 10.1055/s-0028-1084581

M3 - Abstract

VL - 74

SP - 1101

BT - Proceedings of the 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF, Athens, Greece, 3-8 August 2008

ER -

Grevenstuk T, Van DHJJJ, Vervoort JJM, Romano A. Chemical investigation of Pinguicula lusitanica by HPLC-MS and HPLC-SPE-NMR. In Proceedings of the 7th Joint Meeting of GA, AFERP, ASP, PSE & SIF, Athens, Greece, 3-8 August 2008. Vol. 74. 2008. p. 1101 https://doi.org/10.1055/s-0028-1084581