Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia

Aline Aparecida Saldanha, Lucas Fernandes Do Carmo, Sara Batista Do Nascimento, Natália Alves de Matos, Clarice de Carvalho Veloso, Ana Hortência Fonsêca Castro, Ric C.H. de Vos, André Klein, João Máximo de Siqueira, Carlos Alexandre Carollo, Thalita Vieira Do Nascimento, Mônica Cristina Toffoli-Kadri, Adriana Cristina Soares*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)

Abstract

An ethnopharmacological survey indicates that the genus Byrsonima has some medicinal species that are commonly found in the Brazilian Cerrado and has been used as an anti-inflammatory and for gastroduodenal disorders. The aim of this study was to evaluate the anti-inflammatory and antioxidant activity along with qualitative chemical characterization of the methanolic extract of the leaves of Byrsonima verbascifolia (BvME) obtained by exhaustive percolation. The data from the chemical analyses by liquid chromatography–mass spectrometry led to tentative identification of 42 compounds belonging to proanthocyanidins, galloyl quinic acid derivatives, flavonoids, and triterpene glycoside derivatives. BvME contain flavonoids and show an antioxidative activity. The methanolic extract administered intraperitoneally at doses of 50, 100, or 300 mg/kg showed a significant reduction in paw edema and modulated the neutrophil influx in a mouse model. Furthermore, the anti-edematogenic activity of the extract provided in smaller doses (12.5 and 25 mg/kg) was also demonstrated in a mouse paw edema model. The extract inhibited NO production by macrophages induced by lipopolysaccharide. We presume that the anti-inflammatory effects of BvME are due to a combination of compounds present in B. verbascifolia, including catechins (procyanidins), flavonoids, and triterpene glycosides and that these anti-inflammatory actions should be mediated, at least partly, through the inhibition of NO production. This study supports and validates the ethnopharmacological uses of B. verbascifolia as an anti-inflammatory.

Original languageEnglish
Pages (from-to)760-768
JournalJournal of Natural Medicines
Volume70
Issue number4
DOIs
Publication statusPublished - 2016

Fingerprint

Anti-Inflammatory Agents
Flavonoids
Proanthocyanidins
Chemical analysis
Triterpenes
Glycosides
Edema
Quinic Acid
Derivatives
Catechin
Macrophages
Spectrometry
Lipopolysaccharides
Spectrum Analysis
Neutrophils
Antioxidants
Liquids

Keywords

  • Anti-inflammation
  • Byrsonima verbascifolia leaves
  • Flavonoids
  • Mass spectrometry
  • Nitric oxide

Cite this

Saldanha, A. A., Do Carmo, L. F., Do Nascimento, S. B., de Matos, N. A., de Carvalho Veloso, C., Castro, A. H. F., ... Soares, A. C. (2016). Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia. Journal of Natural Medicines, 70(4), 760-768. https://doi.org/10.1007/s11418-016-1011-3
Saldanha, Aline Aparecida ; Do Carmo, Lucas Fernandes ; Do Nascimento, Sara Batista ; de Matos, Natália Alves ; de Carvalho Veloso, Clarice ; Castro, Ana Hortência Fonsêca ; de Vos, Ric C.H. ; Klein, André ; de Siqueira, João Máximo ; Carollo, Carlos Alexandre ; Do Nascimento, Thalita Vieira ; Toffoli-Kadri, Mônica Cristina ; Soares, Adriana Cristina. / Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia. In: Journal of Natural Medicines. 2016 ; Vol. 70, No. 4. pp. 760-768.
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abstract = "An ethnopharmacological survey indicates that the genus Byrsonima has some medicinal species that are commonly found in the Brazilian Cerrado and has been used as an anti-inflammatory and for gastroduodenal disorders. The aim of this study was to evaluate the anti-inflammatory and antioxidant activity along with qualitative chemical characterization of the methanolic extract of the leaves of Byrsonima verbascifolia (BvME) obtained by exhaustive percolation. The data from the chemical analyses by liquid chromatography–mass spectrometry led to tentative identification of 42 compounds belonging to proanthocyanidins, galloyl quinic acid derivatives, flavonoids, and triterpene glycoside derivatives. BvME contain flavonoids and show an antioxidative activity. The methanolic extract administered intraperitoneally at doses of 50, 100, or 300 mg/kg showed a significant reduction in paw edema and modulated the neutrophil influx in a mouse model. Furthermore, the anti-edematogenic activity of the extract provided in smaller doses (12.5 and 25 mg/kg) was also demonstrated in a mouse paw edema model. The extract inhibited NO production by macrophages induced by lipopolysaccharide. We presume that the anti-inflammatory effects of BvME are due to a combination of compounds present in B. verbascifolia, including catechins (procyanidins), flavonoids, and triterpene glycosides and that these anti-inflammatory actions should be mediated, at least partly, through the inhibition of NO production. This study supports and validates the ethnopharmacological uses of B. verbascifolia as an anti-inflammatory.",
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Saldanha, AA, Do Carmo, LF, Do Nascimento, SB, de Matos, NA, de Carvalho Veloso, C, Castro, AHF, de Vos, RCH, Klein, A, de Siqueira, JM, Carollo, CA, Do Nascimento, TV, Toffoli-Kadri, MC & Soares, AC 2016, 'Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia', Journal of Natural Medicines, vol. 70, no. 4, pp. 760-768. https://doi.org/10.1007/s11418-016-1011-3

Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia. / Saldanha, Aline Aparecida; Do Carmo, Lucas Fernandes; Do Nascimento, Sara Batista; de Matos, Natália Alves; de Carvalho Veloso, Clarice; Castro, Ana Hortência Fonsêca; de Vos, Ric C.H.; Klein, André; de Siqueira, João Máximo; Carollo, Carlos Alexandre; Do Nascimento, Thalita Vieira; Toffoli-Kadri, Mônica Cristina; Soares, Adriana Cristina.

In: Journal of Natural Medicines, Vol. 70, No. 4, 2016, p. 760-768.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Saldanha, Aline Aparecida

AU - Do Carmo, Lucas Fernandes

AU - Do Nascimento, Sara Batista

AU - de Matos, Natália Alves

AU - de Carvalho Veloso, Clarice

AU - Castro, Ana Hortência Fonsêca

AU - de Vos, Ric C.H.

AU - Klein, André

AU - de Siqueira, João Máximo

AU - Carollo, Carlos Alexandre

AU - Do Nascimento, Thalita Vieira

AU - Toffoli-Kadri, Mônica Cristina

AU - Soares, Adriana Cristina

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AB - An ethnopharmacological survey indicates that the genus Byrsonima has some medicinal species that are commonly found in the Brazilian Cerrado and has been used as an anti-inflammatory and for gastroduodenal disorders. The aim of this study was to evaluate the anti-inflammatory and antioxidant activity along with qualitative chemical characterization of the methanolic extract of the leaves of Byrsonima verbascifolia (BvME) obtained by exhaustive percolation. The data from the chemical analyses by liquid chromatography–mass spectrometry led to tentative identification of 42 compounds belonging to proanthocyanidins, galloyl quinic acid derivatives, flavonoids, and triterpene glycoside derivatives. BvME contain flavonoids and show an antioxidative activity. The methanolic extract administered intraperitoneally at doses of 50, 100, or 300 mg/kg showed a significant reduction in paw edema and modulated the neutrophil influx in a mouse model. Furthermore, the anti-edematogenic activity of the extract provided in smaller doses (12.5 and 25 mg/kg) was also demonstrated in a mouse paw edema model. The extract inhibited NO production by macrophages induced by lipopolysaccharide. We presume that the anti-inflammatory effects of BvME are due to a combination of compounds present in B. verbascifolia, including catechins (procyanidins), flavonoids, and triterpene glycosides and that these anti-inflammatory actions should be mediated, at least partly, through the inhibition of NO production. This study supports and validates the ethnopharmacological uses of B. verbascifolia as an anti-inflammatory.

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Saldanha AA, Do Carmo LF, Do Nascimento SB, de Matos NA, de Carvalho Veloso C, Castro AHF et al. Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia. Journal of Natural Medicines. 2016;70(4):760-768. https://doi.org/10.1007/s11418-016-1011-3