Chemical composition and anti-inflammatory activity of the leaves of Byrsonima verbascifolia

Aline Aparecida Saldanha, Lucas Fernandes Do Carmo, Sara Batista Do Nascimento, Natália Alves de Matos, Clarice de Carvalho Veloso, Ana Hortência Fonsêca Castro, Ric C.H. de Vos, André Klein, João Máximo de Siqueira, Carlos Alexandre Carollo, Thalita Vieira Do Nascimento, Mônica Cristina Toffoli-Kadri, Adriana Cristina Soares*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

7 Citations (Scopus)


An ethnopharmacological survey indicates that the genus Byrsonima has some medicinal species that are commonly found in the Brazilian Cerrado and has been used as an anti-inflammatory and for gastroduodenal disorders. The aim of this study was to evaluate the anti-inflammatory and antioxidant activity along with qualitative chemical characterization of the methanolic extract of the leaves of Byrsonima verbascifolia (BvME) obtained by exhaustive percolation. The data from the chemical analyses by liquid chromatography–mass spectrometry led to tentative identification of 42 compounds belonging to proanthocyanidins, galloyl quinic acid derivatives, flavonoids, and triterpene glycoside derivatives. BvME contain flavonoids and show an antioxidative activity. The methanolic extract administered intraperitoneally at doses of 50, 100, or 300 mg/kg showed a significant reduction in paw edema and modulated the neutrophil influx in a mouse model. Furthermore, the anti-edematogenic activity of the extract provided in smaller doses (12.5 and 25 mg/kg) was also demonstrated in a mouse paw edema model. The extract inhibited NO production by macrophages induced by lipopolysaccharide. We presume that the anti-inflammatory effects of BvME are due to a combination of compounds present in B. verbascifolia, including catechins (procyanidins), flavonoids, and triterpene glycosides and that these anti-inflammatory actions should be mediated, at least partly, through the inhibition of NO production. This study supports and validates the ethnopharmacological uses of B. verbascifolia as an anti-inflammatory.

Original languageEnglish
Pages (from-to)760-768
JournalJournal of Natural Medicines
Issue number4
Publication statusPublished - 2016


  • Anti-inflammation
  • Byrsonima verbascifolia leaves
  • Flavonoids
  • Mass spectrometry
  • Nitric oxide

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