Chemical characterization and wound healing property of a β-D-glucan from edible mushroom Piptoporus betulinus

Liana Inara de Jesus, Fhernanda R. Smiderle, Andrea C. Ruthes, Francisco Vilaplana, Fernando Tonholi Dal'Lin, Daniele Maria-Ferreira, Maria Fernanda Werner, Leo J.L.D. Van Griensven, Marcello Iacomini*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

A water-soluble β-D-glucan was obtained from fruiting bodies of Piptoporus betulinus, by hot aqueous extraction followed by freeze-thawing procedure and dialysis. Its molar mass distribution and conformational behavior in solution was assessed by size-exclusion chromatography coupled with multiangle laser light scattering, showing a polysaccharide with an average molecular weight of 2.5 × 105 Da with a random coil conformation for molecular weights below 1 × 106 Da. Typical signals of β-(1 → 3)-linkages were observed in NMR spectrum (δ 102.7/4.76; 102.8/4.74; 102.9/4.52; and δ 85.1/3.78; 85.0/3.77) and also signals of O-6 substitution at δ 69.2/4.22 and 69.2/3.87. The analysis of partially O-methylated alditol acetates corroborates the NMR results, indicating the presence of a β-D-glucan with a main chain (1 → 3)-linked, substituted at O-6 by single-units of glucose. The β-D-glucan showed no toxicity on human colon carcinoma cell line (Caco-2) up to 1000 μg mL-1 and promoted cell migration on in vitro scratch assay, demonstrating a potential wound healing capacity.
Original languageEnglish
Pages (from-to)1361-1366
JournalInternational Journal of Biological Macromolecules
Volume117
Early online date20 Dec 2017
DOIs
Publication statusPublished - 1 Oct 2018

Keywords

  • Random coil
  • Wound healing
  • β-D-glucan

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    de Jesus, L. I., Smiderle, F. R., Ruthes, A. C., Vilaplana, F., Dal'Lin, F. T., Maria-Ferreira, D., Werner, M. F., Van Griensven, L. J. L. D., & Iacomini, M. (2018). Chemical characterization and wound healing property of a β-D-glucan from edible mushroom Piptoporus betulinus. International Journal of Biological Macromolecules, 117, 1361-1366. https://doi.org/10.1016/j.ijbiomac.2017.12.107