Chemical changes and increased degradability of wheat straw and oak wood chips treated with the white rot fungi Ceriporiopsis subvermispora and Lentinula edodes

Sandra J.A. van Kuijk*, Anton S.M. Sonnenberg, Johan J.P. Baars, Wouter H. Hendriks, José C. del Río, Jorge Rencoret, Ana Gutiérrez, Norbert C.A. de Ruijter, John W. Cone

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

Wheat straw and oak wood chips were incubated with Ceriporiopsis subvermispora and Lentinula edodes for 8 weeks. Samples from the fungal treated substrates were collected every week for chemical characterization. L. edodes continuously grew during the 8 weeks on both wheat straw and oak wood chips, as determined by the ergosterol mass fraction of the dry biomass. C. subvermispora colonized both substrates during the first week, stopped growing on oak wood chips, and resumed growth after 6 weeks on wheat straw. Detergent fiber analysis and pyrolysis coupled to gas chromatography/mass spectrometry showed a selective lignin degradation in wheat straw, although some carbohydrates were also degraded. L. edodes continuously degraded lignin and hemicelluloses in wheat straw while C. subvermispora degraded lignin and hemicelluloses only during the first 5 weeks of treatment after which cellulose degradation started. Both fungi selectively degraded lignin in wood chips. After 4 weeks of treatment, no significant changes in chemical composition were detected. In contrast to L. edodes, C. subvermispora produced alkylitaconic acids during fungal treatment, which paralleled the degradation and modification of lignin indicating the importance of these compounds in delignification. Light microscopy visualized a dense structure of wood chips which was difficult to penetrate by the fungi, explaining the relative lower lignin degradation compared to wheat straw measured by chemical analysis. All these changes resulted in an increased in in vitro rumen degradability of wheat straw and oak wood chips. In addition, more glucose and xylose were released after enzymatic saccharification of fungal treated wheat straw compared to untreated material.
Original languageEnglish
Pages (from-to)381-391
JournalBiomass and Bioenergy
Volume105
DOIs
Publication statusPublished - 2017

Fingerprint

Ceriporiopsis subvermispora
Lentinula edodes
white-rot fungi
wood chips
Straw
wheat straw
Fungi
straw
Wood
Quercus
wheat
Lignin
fungus
lignin
Degradation
degradation
hemicellulose
delignification
ergosterol
substrate

Keywords

  • Alkylitaconic acids
  • Enzymatic saccharification
  • Fungal treatment
  • In vitro rumen degradability
  • Lignocellulosic biomass
  • Selective lignin degradation

Cite this

@article{33c5a311210f417c8b674a207b470b49,
title = "Chemical changes and increased degradability of wheat straw and oak wood chips treated with the white rot fungi Ceriporiopsis subvermispora and Lentinula edodes",
abstract = "Wheat straw and oak wood chips were incubated with Ceriporiopsis subvermispora and Lentinula edodes for 8 weeks. Samples from the fungal treated substrates were collected every week for chemical characterization. L. edodes continuously grew during the 8 weeks on both wheat straw and oak wood chips, as determined by the ergosterol mass fraction of the dry biomass. C. subvermispora colonized both substrates during the first week, stopped growing on oak wood chips, and resumed growth after 6 weeks on wheat straw. Detergent fiber analysis and pyrolysis coupled to gas chromatography/mass spectrometry showed a selective lignin degradation in wheat straw, although some carbohydrates were also degraded. L. edodes continuously degraded lignin and hemicelluloses in wheat straw while C. subvermispora degraded lignin and hemicelluloses only during the first 5 weeks of treatment after which cellulose degradation started. Both fungi selectively degraded lignin in wood chips. After 4 weeks of treatment, no significant changes in chemical composition were detected. In contrast to L. edodes, C. subvermispora produced alkylitaconic acids during fungal treatment, which paralleled the degradation and modification of lignin indicating the importance of these compounds in delignification. Light microscopy visualized a dense structure of wood chips which was difficult to penetrate by the fungi, explaining the relative lower lignin degradation compared to wheat straw measured by chemical analysis. All these changes resulted in an increased in in vitro rumen degradability of wheat straw and oak wood chips. In addition, more glucose and xylose were released after enzymatic saccharification of fungal treated wheat straw compared to untreated material.",
keywords = "Alkylitaconic acids, Enzymatic saccharification, Fungal treatment, In vitro rumen degradability, Lignocellulosic biomass, Selective lignin degradation",
author = "{van Kuijk}, {Sandra J.A.} and Sonnenberg, {Anton S.M.} and Baars, {Johan J.P.} and Hendriks, {Wouter H.} and {del R{\'i}o}, {Jos{\'e} C.} and Jorge Rencoret and Ana Guti{\'e}rrez and {de Ruijter}, {Norbert C.A.} and Cone, {John W.}",
year = "2017",
doi = "10.1016/j.biombioe.2017.07.003",
language = "English",
volume = "105",
pages = "381--391",
journal = "Biomass and Bioenergy",
issn = "0961-9534",
publisher = "Elsevier",

}

Chemical changes and increased degradability of wheat straw and oak wood chips treated with the white rot fungi Ceriporiopsis subvermispora and Lentinula edodes. / van Kuijk, Sandra J.A.; Sonnenberg, Anton S.M.; Baars, Johan J.P.; Hendriks, Wouter H.; del Río, José C.; Rencoret, Jorge; Gutiérrez, Ana; de Ruijter, Norbert C.A.; Cone, John W.

In: Biomass and Bioenergy, Vol. 105, 2017, p. 381-391.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Chemical changes and increased degradability of wheat straw and oak wood chips treated with the white rot fungi Ceriporiopsis subvermispora and Lentinula edodes

AU - van Kuijk, Sandra J.A.

AU - Sonnenberg, Anton S.M.

AU - Baars, Johan J.P.

AU - Hendriks, Wouter H.

AU - del Río, José C.

AU - Rencoret, Jorge

AU - Gutiérrez, Ana

AU - de Ruijter, Norbert C.A.

AU - Cone, John W.

PY - 2017

Y1 - 2017

N2 - Wheat straw and oak wood chips were incubated with Ceriporiopsis subvermispora and Lentinula edodes for 8 weeks. Samples from the fungal treated substrates were collected every week for chemical characterization. L. edodes continuously grew during the 8 weeks on both wheat straw and oak wood chips, as determined by the ergosterol mass fraction of the dry biomass. C. subvermispora colonized both substrates during the first week, stopped growing on oak wood chips, and resumed growth after 6 weeks on wheat straw. Detergent fiber analysis and pyrolysis coupled to gas chromatography/mass spectrometry showed a selective lignin degradation in wheat straw, although some carbohydrates were also degraded. L. edodes continuously degraded lignin and hemicelluloses in wheat straw while C. subvermispora degraded lignin and hemicelluloses only during the first 5 weeks of treatment after which cellulose degradation started. Both fungi selectively degraded lignin in wood chips. After 4 weeks of treatment, no significant changes in chemical composition were detected. In contrast to L. edodes, C. subvermispora produced alkylitaconic acids during fungal treatment, which paralleled the degradation and modification of lignin indicating the importance of these compounds in delignification. Light microscopy visualized a dense structure of wood chips which was difficult to penetrate by the fungi, explaining the relative lower lignin degradation compared to wheat straw measured by chemical analysis. All these changes resulted in an increased in in vitro rumen degradability of wheat straw and oak wood chips. In addition, more glucose and xylose were released after enzymatic saccharification of fungal treated wheat straw compared to untreated material.

AB - Wheat straw and oak wood chips were incubated with Ceriporiopsis subvermispora and Lentinula edodes for 8 weeks. Samples from the fungal treated substrates were collected every week for chemical characterization. L. edodes continuously grew during the 8 weeks on both wheat straw and oak wood chips, as determined by the ergosterol mass fraction of the dry biomass. C. subvermispora colonized both substrates during the first week, stopped growing on oak wood chips, and resumed growth after 6 weeks on wheat straw. Detergent fiber analysis and pyrolysis coupled to gas chromatography/mass spectrometry showed a selective lignin degradation in wheat straw, although some carbohydrates were also degraded. L. edodes continuously degraded lignin and hemicelluloses in wheat straw while C. subvermispora degraded lignin and hemicelluloses only during the first 5 weeks of treatment after which cellulose degradation started. Both fungi selectively degraded lignin in wood chips. After 4 weeks of treatment, no significant changes in chemical composition were detected. In contrast to L. edodes, C. subvermispora produced alkylitaconic acids during fungal treatment, which paralleled the degradation and modification of lignin indicating the importance of these compounds in delignification. Light microscopy visualized a dense structure of wood chips which was difficult to penetrate by the fungi, explaining the relative lower lignin degradation compared to wheat straw measured by chemical analysis. All these changes resulted in an increased in in vitro rumen degradability of wheat straw and oak wood chips. In addition, more glucose and xylose were released after enzymatic saccharification of fungal treated wheat straw compared to untreated material.

KW - Alkylitaconic acids

KW - Enzymatic saccharification

KW - Fungal treatment

KW - In vitro rumen degradability

KW - Lignocellulosic biomass

KW - Selective lignin degradation

U2 - 10.1016/j.biombioe.2017.07.003

DO - 10.1016/j.biombioe.2017.07.003

M3 - Article

VL - 105

SP - 381

EP - 391

JO - Biomass and Bioenergy

JF - Biomass and Bioenergy

SN - 0961-9534

ER -