Characterizing emulsion properties of microalgal and cyanobacterial protein isolates

Emma Teuling, Johan W. Schrama, Harry Gruppen, Peter A. Wierenga*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

15 Citations (Scopus)


Photosynthetic unicellular sources contain a large variety of proteins. The types of proteins vary between different microalgae and cyanobacteria. The aim was to study the effect of the variation in proteins and in non-proteinaceous components present in various unicellular protein isolates on their emulsion behavior. Algae soluble protein isolates (ASPIs, 66–77% w/w protein) of Nannochloropsis gaditana, Tetraselmis impellucida and Arthrospira (Spirulina) maxima were studied, using commercially available WPI as a reference (93% w/w protein). All protein isolates could form emulsions stable against creaming (d 3,2 0.2–0.3 μm) at pH 8.0. The amount of each ASPI needed (C cr ; on protein basis) to form these stable emulsions varied between the isolates, but was within the range of proteins from both similar (photosynthetic) sources (algae and sugar beet leaves) and other protein sources (dairy, legume and egg). Minor differences were observed in the pH dependence of flocculation amongst the ASPI stabilized emulsions. For the ASPIs, the expected correlation between interfacial and molecular properties (adsorption rate constant and ζ-potential) and the emulsion behavior (C cr and droplet size as a function of pH) was absent.

Original languageEnglish
Article number101471
JournalAlgal Research
Early online date19 Mar 2019
Publication statusPublished - May 2019


  • Critical protein concentration
  • Cyanobacteria
  • Emulsion behavior
  • Interfacial properties
  • Microalgae
  • Rubisco


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