Characterization of thirty two microsatellite loci for three Atlanto-Mediterranean echinoderm species

Alex Garcia-Cisneros, Claudio Valero-Jiménez, Creu Palacín, Rocío Pérez-Portela*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

Thirty two microsatellites were optimized from 454 pyrosequencing libraries for three Atlanto-Mediterranean echinoderms: Coscinasterias tenuispina, Echinaster sepositus and Arbacia lixula. We observed different frequency of microsatellite types (di-, tri-, tetra- and penta-nucleotide) throughout the genome of the species, but no significant differences were observed in allele richness among different microsatellite repeats. No loci showed linkage disequilibrium. Heterozygosity deficit and departure from Hardy-Weinberg equilibrium were observed for some loci, in two species, probably due to high levels of inbreeding. Heterozygosity excess observed in C. tenuispina could be explained by selection against homozygotes and/or outcrossing.

Original languageEnglish
Pages (from-to)749-753
Number of pages5
JournalConservation Genetics Resources
Volume5
Issue number3
DOIs
Publication statusPublished - Sep 2013

Keywords

  • Clonality
  • Conservation
  • Inbreeding
  • Pyrosequencing
  • Sea urchin
  • Starfish

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