Characterization of the Theileria parva sporozoite proteome

James Nyagwange, Edwin Tijhaar, Nicola Ternette, Fredrick Mobegi, Kyle Tretina, Joana C. Silva, Roger Pelle, Vishvanath Nene

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

East Coast fever is a lymphoproliferative disease caused by the tick-borne protozoan parasite Theileria parva. The sporozoite stage of this parasite, harboured and released from the salivary glands of the tick Rhipicephalus appendiculatus during feeding, invades and establishes infection in bovine lymphocytes. Blocking this initial stage of invasion presents a promising vaccine strategy for control of East Coast fever and can in part be achieved by targeting the major sporozoite surface protein p67. To support research on the biology of T. parva and the identification of additional candidate vaccine antigens, we report on the sporozoite proteome as defined by LC-MS/MS analysis. In total, 4780 proteins were identified in an enriched preparation of sporozoites. Of these, 2007 were identified as T. parva proteins, representing close to 50% of the total predicted parasite proteome. The remaining 2773 proteins were derived from the tick vector. The identified sporozoite proteins include a set of known T. parva antigens targeted by antibodies and cytotoxic T cells from cattle that are immune to East Coast fever. We also identified proteins predicted to be orthologs of Plasmodium falciparum sporozoite surface molecules and invasion organelle proteins, and proteins that may contribute to the phenomenon of bovine lymphocyte transformation. Overall, these data establish a protein expression profile of T. parva sporozoites as an important starting point for further study of a parasitic species which has considerable agricultural impact.
Original languageEnglish
Pages (from-to)265-273
JournalInternational Journal for Parasitology
Volume48
Issue number3-4
Early online date2017
DOIs
Publication statusPublished - Mar 2018

Fingerprint

Theileria parva
Sporozoites
Proteome
Theileriasis
Proteins
Parasites
Ticks
Vaccines
Rhipicephalus
Tick-Borne Diseases
Viral Tumor Antigens
Plasmodium falciparum
Lymphocyte Activation
Salivary Glands
Organelles
Membrane Proteins
Lymphocytes
T-Lymphocytes
Antigens

Keywords

  • Antigens
  • East Coast fever
  • MudPIT
  • Proteomics
  • Sporozoites
  • Theileria

Cite this

Nyagwange, J., Tijhaar, E., Ternette, N., Mobegi, F., Tretina, K., Silva, J. C., ... Nene, V. (2018). Characterization of the Theileria parva sporozoite proteome. International Journal for Parasitology, 48(3-4), 265-273. https://doi.org/10.1016/j.ijpara.2017.09.007
Nyagwange, James ; Tijhaar, Edwin ; Ternette, Nicola ; Mobegi, Fredrick ; Tretina, Kyle ; Silva, Joana C. ; Pelle, Roger ; Nene, Vishvanath. / Characterization of the Theileria parva sporozoite proteome. In: International Journal for Parasitology. 2018 ; Vol. 48, No. 3-4. pp. 265-273.
@article{f7f8f7b7acda4ec6bdd4318ff8281a57,
title = "Characterization of the Theileria parva sporozoite proteome",
abstract = "East Coast fever is a lymphoproliferative disease caused by the tick-borne protozoan parasite Theileria parva. The sporozoite stage of this parasite, harboured and released from the salivary glands of the tick Rhipicephalus appendiculatus during feeding, invades and establishes infection in bovine lymphocytes. Blocking this initial stage of invasion presents a promising vaccine strategy for control of East Coast fever and can in part be achieved by targeting the major sporozoite surface protein p67. To support research on the biology of T. parva and the identification of additional candidate vaccine antigens, we report on the sporozoite proteome as defined by LC-MS/MS analysis. In total, 4780 proteins were identified in an enriched preparation of sporozoites. Of these, 2007 were identified as T. parva proteins, representing close to 50{\%} of the total predicted parasite proteome. The remaining 2773 proteins were derived from the tick vector. The identified sporozoite proteins include a set of known T. parva antigens targeted by antibodies and cytotoxic T cells from cattle that are immune to East Coast fever. We also identified proteins predicted to be orthologs of Plasmodium falciparum sporozoite surface molecules and invasion organelle proteins, and proteins that may contribute to the phenomenon of bovine lymphocyte transformation. Overall, these data establish a protein expression profile of T. parva sporozoites as an important starting point for further study of a parasitic species which has considerable agricultural impact.",
keywords = "Antigens, East Coast fever, MudPIT, Proteomics, Sporozoites, Theileria",
author = "James Nyagwange and Edwin Tijhaar and Nicola Ternette and Fredrick Mobegi and Kyle Tretina and Silva, {Joana C.} and Roger Pelle and Vishvanath Nene",
year = "2018",
month = "3",
doi = "10.1016/j.ijpara.2017.09.007",
language = "English",
volume = "48",
pages = "265--273",
journal = "International Journal for Parasitology",
issn = "0020-7519",
publisher = "Elsevier",
number = "3-4",

}

Nyagwange, J, Tijhaar, E, Ternette, N, Mobegi, F, Tretina, K, Silva, JC, Pelle, R & Nene, V 2018, 'Characterization of the Theileria parva sporozoite proteome' International Journal for Parasitology, vol. 48, no. 3-4, pp. 265-273. https://doi.org/10.1016/j.ijpara.2017.09.007

Characterization of the Theileria parva sporozoite proteome. / Nyagwange, James; Tijhaar, Edwin; Ternette, Nicola; Mobegi, Fredrick; Tretina, Kyle; Silva, Joana C.; Pelle, Roger; Nene, Vishvanath.

In: International Journal for Parasitology, Vol. 48, No. 3-4, 03.2018, p. 265-273.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Characterization of the Theileria parva sporozoite proteome

AU - Nyagwange, James

AU - Tijhaar, Edwin

AU - Ternette, Nicola

AU - Mobegi, Fredrick

AU - Tretina, Kyle

AU - Silva, Joana C.

AU - Pelle, Roger

AU - Nene, Vishvanath

PY - 2018/3

Y1 - 2018/3

N2 - East Coast fever is a lymphoproliferative disease caused by the tick-borne protozoan parasite Theileria parva. The sporozoite stage of this parasite, harboured and released from the salivary glands of the tick Rhipicephalus appendiculatus during feeding, invades and establishes infection in bovine lymphocytes. Blocking this initial stage of invasion presents a promising vaccine strategy for control of East Coast fever and can in part be achieved by targeting the major sporozoite surface protein p67. To support research on the biology of T. parva and the identification of additional candidate vaccine antigens, we report on the sporozoite proteome as defined by LC-MS/MS analysis. In total, 4780 proteins were identified in an enriched preparation of sporozoites. Of these, 2007 were identified as T. parva proteins, representing close to 50% of the total predicted parasite proteome. The remaining 2773 proteins were derived from the tick vector. The identified sporozoite proteins include a set of known T. parva antigens targeted by antibodies and cytotoxic T cells from cattle that are immune to East Coast fever. We also identified proteins predicted to be orthologs of Plasmodium falciparum sporozoite surface molecules and invasion organelle proteins, and proteins that may contribute to the phenomenon of bovine lymphocyte transformation. Overall, these data establish a protein expression profile of T. parva sporozoites as an important starting point for further study of a parasitic species which has considerable agricultural impact.

AB - East Coast fever is a lymphoproliferative disease caused by the tick-borne protozoan parasite Theileria parva. The sporozoite stage of this parasite, harboured and released from the salivary glands of the tick Rhipicephalus appendiculatus during feeding, invades and establishes infection in bovine lymphocytes. Blocking this initial stage of invasion presents a promising vaccine strategy for control of East Coast fever and can in part be achieved by targeting the major sporozoite surface protein p67. To support research on the biology of T. parva and the identification of additional candidate vaccine antigens, we report on the sporozoite proteome as defined by LC-MS/MS analysis. In total, 4780 proteins were identified in an enriched preparation of sporozoites. Of these, 2007 were identified as T. parva proteins, representing close to 50% of the total predicted parasite proteome. The remaining 2773 proteins were derived from the tick vector. The identified sporozoite proteins include a set of known T. parva antigens targeted by antibodies and cytotoxic T cells from cattle that are immune to East Coast fever. We also identified proteins predicted to be orthologs of Plasmodium falciparum sporozoite surface molecules and invasion organelle proteins, and proteins that may contribute to the phenomenon of bovine lymphocyte transformation. Overall, these data establish a protein expression profile of T. parva sporozoites as an important starting point for further study of a parasitic species which has considerable agricultural impact.

KW - Antigens

KW - East Coast fever

KW - MudPIT

KW - Proteomics

KW - Sporozoites

KW - Theileria

U2 - 10.1016/j.ijpara.2017.09.007

DO - 10.1016/j.ijpara.2017.09.007

M3 - Article

VL - 48

SP - 265

EP - 273

JO - International Journal for Parasitology

JF - International Journal for Parasitology

SN - 0020-7519

IS - 3-4

ER -