Characterization of the chlorate reductase from Pseudomonas chloritidismutans

A.F.W.M. Wolterink, E. Schiltz, P.L. Hagedoorn, W.R. Hagen, S.W.M. Kengen, A.J.M. Stams

Research output: Contribution to journalArticleAcademicpeer-review

40 Citations (Scopus)

Abstract

A chlorate reductase has been purified from the chlorate-reducing strain Pseudomonas chloritidismutans. Comparison with the periplasmic (per)chlorate reductase of strain GR-1 showed that the cytoplasmic chlorate reductase of P. chloritidismutans reduced only chlorate and bromate. Differences were also found in N-terminal sequences, molecular weight, and subunit composition. Metal analysis and electron paramagnetic resonance measurements showed the presence of iron and molybdenum, which are also found in other dissimilatory oxyanion reductases
Original languageEnglish
Pages (from-to)3210-3213
JournalJournal of Bacteriology
Volume185
Issue number10
DOIs
Publication statusPublished - 2003

Keywords

  • desulfovibrio-vulgaris hildenborough
  • nitrate reductase
  • paracoccus-denitrificans
  • haloarcula-marismortui
  • energy transduction
  • oxide reductase
  • purification
  • dehydrogenase
  • (per)chlorate
  • enzymes

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