Characterization of the CD8(+)T cell responses directed against respiratory syncytial virus during primary and secondary infection in C57BL/6 mice

M.V. Lukens, E.A.W. Claassen, P.M.A. de Graaff, M.E.A. van Dijk, P. Hoogerhout, M. Toebes, T.N. Schumacher, R.G. van der Most, J.L.L. Kimpen, G.M. van Bleek

    Research output: Contribution to journalArticleAcademicpeer-review

    48 Citations (Scopus)

    Abstract

    The BALB/c mouse model for human respiratory syncytial virus infection has contributed significantly to our understanding of the relative role for CD4+ and CD8+ T cells to immune protection and pathogenic immune responses. To enable comparison of RSV-specific T cell responses in different mouse strains and allow dissection of immune mechanisms by using transgenic and knockout mice that are mostly available on a C57BL/6 background, we characterized the specificity, level and functional capabilities of CD8+ T cells during primary and secondary responses in lung parenchyma, airways and spleens of C57BL/6 mice. During the primary response, epitopes were recognized originating from the matrix, fusion, nucleo- and attachment proteins, whereas the secondary response focused predominantly on the matrix epitope. C57BL/6 mice are less permissive for hRSV infection than BALB/c mice, yet we found CD8+ T cell responses in the lungs and bronchoalveolar lavage, comparable to the responses described for BALB/c mice.
    Original languageEnglish
    Pages (from-to)157-168
    JournalVirology
    Volume352
    Issue number1
    DOIs
    Publication statusPublished - 2006

    Keywords

    • balb/c mice
    • antigen presentation
    • interferon-alpha
    • fusion protein
    • inbred mice
    • identification
    • vaccine
    • tract
    • susceptibility
    • eosinophilia

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