Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.

J. Gerritsen; S. Fuentes Enriquez de Salamanca; W. Grievink; L. van Niftrik; B.J. Tindall; H.M. Timmerman; G.T. Rijkers; H. Smidt

doi:10.1099/ijs.0.059543-0

Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.

J. Gerritsen, S. Fuentes Enriquez de Salamanca, W. Grievink, L. van Niftrik, B.J. Tindall, H.M. Timmerman, G.T. Rijkers, H. Smidt

Research output: Contribution to journal › Article › Academic › peer-review

193 Citations (Scopus)

Abstract

A Gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated CRIBT, was isolated from the gastro-intestinal tract of a rat and characterized. The major cellular fatty acids of strain CRIBT were saturated and unsaturated straight chain C12-C19 fatty acids, with C16:0 being the predominant fatty acid. The polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. The only quinone was MK-6. The predominating cell wall sugars were glucose and galactose. The peptidoglycan type of strain CRIBT was A1d lanthionine-direct. The genomic DNA G+C content of strain CRIBT was 28.1 mol %. On the basis of 16S rRNA gene sequence similarity, strain CRIBT was most closely related to a number of Clostridium species, including C. lituseburense (97.2 %), C. glycolicum (96.2 %), C. mayombei (96.2 %), C. bartlettii (96.0 %) and C. irregulare (95.5 %). All these species show very low 16S rRNA gene sequence similarity (

Original language	English
Pages (from-to)	1600-1616
Journal	International Journal of Systematic and Evolutionary Microbiology
Volume	64
Issue number	Pt. 5
DOIs	https://doi.org/10.1099/ijs.0.059543-0
Publication status	Published - 2014

Keywords

ribosomal-rna genes
acid methyl-esters
lipid-composition
deoxyribonucleic-acid
electron microscopy
renaturation rates
dna hybridization
polar lipids
bacteria
chromatography

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Gerritsen, J., Fuentes Enriquez de Salamanca, S., Grievink, W., van Niftrik, L., Tindall, B. J., Timmerman, H. M., Rijkers, G. T., & Smidt, H. (2014). Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov. International Journal of Systematic and Evolutionary Microbiology, 64(Pt. 5), 1600-1616. https://doi.org/10.1099/ijs.0.059543-0

Gerritsen, J. ; Fuentes Enriquez de Salamanca, S. ; Grievink, W. et al. / Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov. In: International Journal of Systematic and Evolutionary Microbiology. 2014 ; Vol. 64, No. Pt. 5. pp. 1600-1616.

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abstract = "A Gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated CRIBT, was isolated from the gastro-intestinal tract of a rat and characterized. The major cellular fatty acids of strain CRIBT were saturated and unsaturated straight chain C12-C19 fatty acids, with C16:0 being the predominant fatty acid. The polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. The only quinone was MK-6. The predominating cell wall sugars were glucose and galactose. The peptidoglycan type of strain CRIBT was A1d lanthionine-direct. The genomic DNA G+C content of strain CRIBT was 28.1 mol %. On the basis of 16S rRNA gene sequence similarity, strain CRIBT was most closely related to a number of Clostridium species, including C. lituseburense (97.2 %), C. glycolicum (96.2 %), C. mayombei (96.2 %), C. bartlettii (96.0 %) and C. irregulare (95.5 %). All these species show very low 16S rRNA gene sequence similarity (",

keywords = "ribosomal-rna genes, acid methyl-esters, lipid-composition, deoxyribonucleic-acid, electron microscopy, renaturation rates, dna hybridization, polar lipids, bacteria, chromatography",

author = "J. Gerritsen and {Fuentes Enriquez de Salamanca}, S. and W. Grievink and {van Niftrik}, L. and B.J. Tindall and H.M. Timmerman and G.T. Rijkers and H. Smidt",

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Gerritsen, J, Fuentes Enriquez de Salamanca, S, Grievink, W, van Niftrik, L, Tindall, BJ, Timmerman, HM, Rijkers, GT & Smidt, H 2014, 'Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.', International Journal of Systematic and Evolutionary Microbiology, vol. 64, no. Pt. 5, pp. 1600-1616. https://doi.org/10.1099/ijs.0.059543-0

Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov. / Gerritsen, J.; Fuentes Enriquez de Salamanca, S.; Grievink, W. et al.
In: International Journal of Systematic and Evolutionary Microbiology, Vol. 64, No. Pt. 5, 2014, p. 1600-1616.

Research output: Contribution to journal › Article › Academic › peer-review

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T1 - Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov.

AU - Gerritsen, J.

AU - Fuentes Enriquez de Salamanca, S.

AU - Grievink, W.

AU - van Niftrik, L.

AU - Tindall, B.J.

AU - Timmerman, H.M.

AU - Rijkers, G.T.

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N2 - A Gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated CRIBT, was isolated from the gastro-intestinal tract of a rat and characterized. The major cellular fatty acids of strain CRIBT were saturated and unsaturated straight chain C12-C19 fatty acids, with C16:0 being the predominant fatty acid. The polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. The only quinone was MK-6. The predominating cell wall sugars were glucose and galactose. The peptidoglycan type of strain CRIBT was A1d lanthionine-direct. The genomic DNA G+C content of strain CRIBT was 28.1 mol %. On the basis of 16S rRNA gene sequence similarity, strain CRIBT was most closely related to a number of Clostridium species, including C. lituseburense (97.2 %), C. glycolicum (96.2 %), C. mayombei (96.2 %), C. bartlettii (96.0 %) and C. irregulare (95.5 %). All these species show very low 16S rRNA gene sequence similarity (

AB - A Gram-positive staining, rod-shaped, non-motile, spore-forming obligately anaerobic bacterium, designated CRIBT, was isolated from the gastro-intestinal tract of a rat and characterized. The major cellular fatty acids of strain CRIBT were saturated and unsaturated straight chain C12-C19 fatty acids, with C16:0 being the predominant fatty acid. The polar lipid profile comprised six glycolipids, four phospholipids and one lipid that did not stain with any of the specific spray reagents used. The only quinone was MK-6. The predominating cell wall sugars were glucose and galactose. The peptidoglycan type of strain CRIBT was A1d lanthionine-direct. The genomic DNA G+C content of strain CRIBT was 28.1 mol %. On the basis of 16S rRNA gene sequence similarity, strain CRIBT was most closely related to a number of Clostridium species, including C. lituseburense (97.2 %), C. glycolicum (96.2 %), C. mayombei (96.2 %), C. bartlettii (96.0 %) and C. irregulare (95.5 %). All these species show very low 16S rRNA gene sequence similarity (

KW - ribosomal-rna genes

KW - acid methyl-esters

KW - lipid-composition

KW - deoxyribonucleic-acid

KW - electron microscopy

KW - renaturation rates

KW - dna hybridization

KW - polar lipids

KW - bacteria

KW - chromatography

U2 - 10.1099/ijs.0.059543-0

DO - 10.1099/ijs.0.059543-0

M3 - Article

SN - 1466-5026

VL - 64

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JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - Pt. 5

ER -

Gerritsen J, Fuentes Enriquez de Salamanca S, Grievink W, van Niftrik L, Tindall BJ, Timmerman HM et al. Characterization of Romboutsia ilealis gen. nov., sp. nov., isolated from the gastro-intestinal tract of a rat and proposal for the reclassification of five closely related members of the genus Clostridium into the genera Romboutsia gen. nov., Intestinibacter gen. nov., Terrisporobacter gen. nov. and Asaccharospora gen. nov. International Journal of Systematic and Evolutionary Microbiology. 2014;64(Pt. 5):1600-1616. doi: 10.1099/ijs.0.059543-0