Characterization of Pseudomonas species causing brown blotch of Agaricus bisporis.

J.M. van der Wolf, P. Kastelein, M.C. Krijger, M.J.A. Hendriks, J.J.P. Baars, J.G.M. Amsing, T.A.J. van der Lee, S. Warris

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademic

Abstract

Bacterial blotch is occasionally causing damage in the production of common mushroom (Agaricus bisporus). The disease is found worldwide and can be caused by different fluorescent Pseudomonas species present in casing material. For identification of the causative agents of blotch in the Netherlands and Belgium, fluorescent Pseudomonas species were isolated from mushrooms with mild or severe brown blotch symptoms collected at six growers. Strains were tested for pathogenicity by streaking the mushroom head with bacterial
slime using a cotton swab and incubation of mushrooms for eight days at 15°C. In total 16 isolates were collected varying in virulence. These, together with 14 other Pseudomonas strains present in different culture collections (LMG and at WUR), including the type strains of P. gingeri, P. tolaasii, P. agaricus and P. reactans were characterized and tentatively identified by multilocus sequence analysis based on four genes (rpoD, rpoB, gyrB, glyA) and by average nucleotide identity using whole genome sequences . We found three species, P. tolaasii, P. gingeri and an unnamed species able to cause severe symptoms whereas other
unnamed species were able to cause mild symptoms. The information can be used for the development of diagnostic tools and in studies on epidemiology and disease management.
Original languageEnglish
Title of host publication19th ISMS Congress proceedings
PublisherInternational Society for Mushroom Science (ISMS)
Volume19
Publication statusPublished - 2016

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Agaricus
mushrooms
Pseudomonas
Pseudomonas gingeri
Pseudomonas tolaasii
Agaricus bisporus
Belgium
epidemiology
Netherlands
growers
disease control
virulence
pathogenicity
cotton
nucleotides
genome
genes

Cite this

van der Wolf, J. M., Kastelein, P., Krijger, M. C., Hendriks, M. J. A., Baars, J. J. P., Amsing, J. G. M., ... Warris, S. (2016). Characterization of Pseudomonas species causing brown blotch of Agaricus bisporis. In 19th ISMS Congress proceedings (Vol. 19). International Society for Mushroom Science (ISMS).
van der Wolf, J.M. ; Kastelein, P. ; Krijger, M.C. ; Hendriks, M.J.A. ; Baars, J.J.P. ; Amsing, J.G.M. ; van der Lee, T.A.J. ; Warris, S. / Characterization of Pseudomonas species causing brown blotch of Agaricus bisporis. 19th ISMS Congress proceedings. Vol. 19 International Society for Mushroom Science (ISMS), 2016.
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abstract = "Bacterial blotch is occasionally causing damage in the production of common mushroom (Agaricus bisporus). The disease is found worldwide and can be caused by different fluorescent Pseudomonas species present in casing material. For identification of the causative agents of blotch in the Netherlands and Belgium, fluorescent Pseudomonas species were isolated from mushrooms with mild or severe brown blotch symptoms collected at six growers. Strains were tested for pathogenicity by streaking the mushroom head with bacterial slime using a cotton swab and incubation of mushrooms for eight days at 15°C. In total 16 isolates were collected varying in virulence. These, together with 14 other Pseudomonas strains present in different culture collections (LMG and at WUR), including the type strains of P. gingeri, P. tolaasii, P. agaricus and P. reactans were characterized and tentatively identified by multilocus sequence analysis based on four genes (rpoD, rpoB, gyrB, glyA) and by average nucleotide identity using whole genome sequences . We found three species, P. tolaasii, P. gingeri and an unnamed species able to cause severe symptoms whereas other unnamed species were able to cause mild symptoms. The information can be used for the development of diagnostic tools and in studies on epidemiology and disease management.",
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van der Wolf, JM, Kastelein, P, Krijger, MC, Hendriks, MJA, Baars, JJP, Amsing, JGM, van der Lee, TAJ & Warris, S 2016, Characterization of Pseudomonas species causing brown blotch of Agaricus bisporis. in 19th ISMS Congress proceedings. vol. 19, International Society for Mushroom Science (ISMS).

Characterization of Pseudomonas species causing brown blotch of Agaricus bisporis. / van der Wolf, J.M.; Kastelein, P.; Krijger, M.C.; Hendriks, M.J.A.; Baars, J.J.P.; Amsing, J.G.M.; van der Lee, T.A.J.; Warris, S.

19th ISMS Congress proceedings. Vol. 19 International Society for Mushroom Science (ISMS), 2016.

Research output: Chapter in Book/Report/Conference proceedingConference paperAcademic

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T1 - Characterization of Pseudomonas species causing brown blotch of Agaricus bisporis.

AU - van der Wolf, J.M.

AU - Kastelein, P.

AU - Krijger, M.C.

AU - Hendriks, M.J.A.

AU - Baars, J.J.P.

AU - Amsing, J.G.M.

AU - van der Lee, T.A.J.

AU - Warris, S.

PY - 2016

Y1 - 2016

N2 - Bacterial blotch is occasionally causing damage in the production of common mushroom (Agaricus bisporus). The disease is found worldwide and can be caused by different fluorescent Pseudomonas species present in casing material. For identification of the causative agents of blotch in the Netherlands and Belgium, fluorescent Pseudomonas species were isolated from mushrooms with mild or severe brown blotch symptoms collected at six growers. Strains were tested for pathogenicity by streaking the mushroom head with bacterial slime using a cotton swab and incubation of mushrooms for eight days at 15°C. In total 16 isolates were collected varying in virulence. These, together with 14 other Pseudomonas strains present in different culture collections (LMG and at WUR), including the type strains of P. gingeri, P. tolaasii, P. agaricus and P. reactans were characterized and tentatively identified by multilocus sequence analysis based on four genes (rpoD, rpoB, gyrB, glyA) and by average nucleotide identity using whole genome sequences . We found three species, P. tolaasii, P. gingeri and an unnamed species able to cause severe symptoms whereas other unnamed species were able to cause mild symptoms. The information can be used for the development of diagnostic tools and in studies on epidemiology and disease management.

AB - Bacterial blotch is occasionally causing damage in the production of common mushroom (Agaricus bisporus). The disease is found worldwide and can be caused by different fluorescent Pseudomonas species present in casing material. For identification of the causative agents of blotch in the Netherlands and Belgium, fluorescent Pseudomonas species were isolated from mushrooms with mild or severe brown blotch symptoms collected at six growers. Strains were tested for pathogenicity by streaking the mushroom head with bacterial slime using a cotton swab and incubation of mushrooms for eight days at 15°C. In total 16 isolates were collected varying in virulence. These, together with 14 other Pseudomonas strains present in different culture collections (LMG and at WUR), including the type strains of P. gingeri, P. tolaasii, P. agaricus and P. reactans were characterized and tentatively identified by multilocus sequence analysis based on four genes (rpoD, rpoB, gyrB, glyA) and by average nucleotide identity using whole genome sequences . We found three species, P. tolaasii, P. gingeri and an unnamed species able to cause severe symptoms whereas other unnamed species were able to cause mild symptoms. The information can be used for the development of diagnostic tools and in studies on epidemiology and disease management.

M3 - Conference paper

VL - 19

BT - 19th ISMS Congress proceedings

PB - International Society for Mushroom Science (ISMS)

ER -

van der Wolf JM, Kastelein P, Krijger MC, Hendriks MJA, Baars JJP, Amsing JGM et al. Characterization of Pseudomonas species causing brown blotch of Agaricus bisporis. In 19th ISMS Congress proceedings. Vol. 19. International Society for Mushroom Science (ISMS). 2016