Characterization of (polyethylene oxide) brushes on glass surfaces and adhesion of Staphylococcus epidermidis

H.J. Kaper, H.J. Busscher, W. Norde

Research output: Contribution to journalArticleAcademicpeer-review

55 Citations (Scopus)


Poly(ethylene oxide) brushes have been covalently bound to glass surfaces and their presence was demonstrated by an increase in water contact angles from fully wettable on glass to advancing contact angles of 54 degrees, with a hysteresis of 32 degrees. In addition, electrophoretic mobilities of glass and brush-coated glass were determined using streaming potential measurements. The dependence of the electrophoretic mobilities on the ionic strength was analyzed in terms of a softlayer model, yielding an electrophoretic softness and fixed charge density of the layer. Brush-coated glass could be distinguished from glass by a 2-3-fold decrease in fixed charge density, while both surfaces were about equally soft. Adhesion of Staphylococcus epidermidis HBH276 to glass in a parallel plate flow chamber was extremely high and after 4 h, 19.0 x 10(6) bacteria were adhering per cm2. In contrast, the organisms did not adhere to brush-coated glass, with numbers below the detection limit, i.e. 0.1 x 10(6) per cm2. These results attest to the great potential of polymer brushes in preventing bacterial adhesion to surfaces.
Original languageEnglish
Pages (from-to)313-324
JournalJournal of Biomaterials Science-Polymer Edition
Issue number4
Publication statusPublished - 2003


  • self-assembled monolayers
  • protein adsorption
  • bacterial adhesion
  • polyethylene oxide
  • polymer brushes
  • model
  • particles


Dive into the research topics of 'Characterization of (polyethylene oxide) brushes on glass surfaces and adhesion of Staphylococcus epidermidis'. Together they form a unique fingerprint.

Cite this