Characterization of PmHS2 glycosyltransferases for the controlled synthesis of heparosan : a precursor of heparin and heparan sulfate

A.A.E. Chavaroche

Research output: Thesisinternal PhD, WU

Abstract

 Heparin (Hep), a highly sulfated and complex glycosaminoglycan polysaccharides, is worldwide used as an anticoagulant compound to prevent blood clotting during surgery. Heparin and its analogue, heparan sulfate (HS), have a large potential for medical applications. Nevertheless, the utilization of Hep/HS-based drugs in new therapeutic settings requires the synthesis of well-defined heparin and heparan sulfate-like molecules. Since neither the extraction from animal derivatives, nor the chemical synthesis, are suitable for the production of a large variety of defined Hep/HS polymers, there is a general interest in developing alternative systems enabling to tightly control Hep/HS synthesis. During the synthesis of heparin and heparan sulfate, the polymerization of the polysaccharide backbone, known as heparosan, determines the chain length and the size distribution of these polymers. Here, the Pasteurella multocida heparosan synthase PmHS2, a bacterial enzyme catalyzing the formation of heparosan polymers, was studied in detail in order to develop methods to control the polymer elongation.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Eggink, Gerrit, Promotor
Award date30 May 2011
Place of PublicationS.l.
Print ISBNs9789085858805
Publication statusPublished - 2011

Keywords

  • industrial microbiology
  • heparin
  • synthesis
  • recombinant proteins

Fingerprint Dive into the research topics of 'Characterization of PmHS2 glycosyltransferases for the controlled synthesis of heparosan : a precursor of heparin and heparan sulfate'. Together they form a unique fingerprint.

Cite this