Characterization of molecular mechanisms underlying the multi-drug-resistant phenotypes of Mycosphaerella graminicola field isolates

Multidrug resistance (MDR) is a common trait developed by many organisms to counteract chemicals and/or drugs used against them. The basic MDR mechanism is relying on an overexpressed efflux transport system that actively expulses the toxic agent outside the cell. In fungi, MDR has been extensively studied in Saccharomyces cerevisiae and Candida albicans, but also plant pathogenic fungi are concerned by this phenomenon. In this study, we confirmed a drug efflux based resistance mechanism in two isolates of the septoria leaf blotch agent Mycosphaerella graminicola. Using next generation sequencing approaches, we could demonstrate that the MgMFS1 gene was strongly overexpressed in both isolates. The mutation(s) responsible for the MDR phenotype was mapped to a 38.5 kb genomic fragment on chromosome VII, covering also the MgMFS1 gene. Precise genetic mapping will then identify the mdr mutation(s) leading to MfMFS1 overexpressio Characterization of molecular mechanisms underlying the multi-drug-resistant phenotypes of Mycosphaerella graminicola field isolates. (PDF Download Available). Available from: http://www.researchgate.net/publication/271765795_Characterization_of_molecular_mechanisms_underlying_the_multi-drug-resistant_phenotypes_of_Mycosphaerella_graminicola_field_isolates [accessed Jun 15, 2015]