Characterization of Dickeya strains isolated from potato grown under hot-climate conditions

L. Tsror; B. Ben-Daniel; L. Chalupowicz; J.M. van der Wolf; S. Lebiush; O. Erlich; O. Dror; V. Barel; E.H. Nijhuis; S. Manulis-Sasson

doi:10.1111/ppa.12030

Characterization of Dickeya strains isolated from potato grown under hot-climate conditions

L. Tsror, B. Ben-Daniel, L. Chalupowicz, J.M. van der Wolf, S. Lebiush, O. Erlich, O. Dror, V. Barel, E.H. Nijhuis, S. Manulis-Sasson

Research output: Contribution to journal › Article › Academic › peer-review

31 Citations (Scopus)

Abstract

Dickeya strains isolated in Israel in 2006–2010 were characterized by dnaX sequence analysis, pulsed-field gel electrophoresis (PFGE), biochemical assays and pectolytic activity, and found to be homogeneous: most of them could be classified as ‘Dickeya solani’. Of the 34 strains isolated from imported seed tubers or potato plants grown from imported seed, 32 were typed as ‘D. solani’ and only two were characterized as Dickeya dianthicola. Biovar typing indicated that all ‘D. solani’ strains were biovar 3. ‘Dickeya solani’ strains were most closely related to Dickeya dadantii subsp. dieffenbachiae according to PFGE and dnaX analyses and both species exhibited high pectolytic activity. Expression levels of two putative virulence genes, pelL (encoding a pectic enzyme) and dspE (encoding a type III effector) were significantly induced in ‘D. solani’ strains isolated from potato plants or tubers grown in hot climates such as the Negev region in Israel, compared to those isolated from seed tubers imported from the Netherlands, France or Germany. Results of this study support the hypothesis that ‘D. solani’ strains isolated in Israel are also clonal; however, they appear to be more virulent than strains isolated in Europe

Original language	English
Pages (from-to)	1097-1105
Journal	Plant Pathology
Volume	62
Issue number	5
DOIs	https://doi.org/10.1111/ppa.12030
Publication status	Published - 2013

Keywords

soft-rot erwinias
chrysanthemi
population
israel
crops
pcr

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@article{8d21e7886bca4984a4b4b6e71616de23,

title = "Characterization of Dickeya strains isolated from potato grown under hot-climate conditions",

abstract = "Dickeya strains isolated in Israel in 2006–2010 were characterized by dnaX sequence analysis, pulsed-field gel electrophoresis (PFGE), biochemical assays and pectolytic activity, and found to be homogeneous: most of them could be classified as {\textquoteleft}Dickeya solani{\textquoteright}. Of the 34 strains isolated from imported seed tubers or potato plants grown from imported seed, 32 were typed as {\textquoteleft}D. solani{\textquoteright} and only two were characterized as Dickeya dianthicola. Biovar typing indicated that all {\textquoteleft}D. solani{\textquoteright} strains were biovar 3. {\textquoteleft}Dickeya solani{\textquoteright} strains were most closely related to Dickeya dadantii subsp. dieffenbachiae according to PFGE and dnaX analyses and both species exhibited high pectolytic activity. Expression levels of two putative virulence genes, pelL (encoding a pectic enzyme) and dspE (encoding a type III effector) were significantly induced in {\textquoteleft}D. solani{\textquoteright} strains isolated from potato plants or tubers grown in hot climates such as the Negev region in Israel, compared to those isolated from seed tubers imported from the Netherlands, France or Germany. Results of this study support the hypothesis that {\textquoteleft}D. solani{\textquoteright} strains isolated in Israel are also clonal; however, they appear to be more virulent than strains isolated in Europe",

keywords = "soft-rot erwinias, chrysanthemi, population, israel, crops, pcr",

author = "L. Tsror and B. Ben-Daniel and L. Chalupowicz and {van der Wolf}, J.M. and S. Lebiush and O. Erlich and O. Dror and V. Barel and E.H. Nijhuis and S. Manulis-Sasson",

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T1 - Characterization of Dickeya strains isolated from potato grown under hot-climate conditions

AU - Tsror, L.

AU - Ben-Daniel, B.

AU - Chalupowicz, L.

AU - van der Wolf, J.M.

AU - Lebiush, S.

AU - Erlich, O.

AU - Dror, O.

AU - Barel, V.

AU - Nijhuis, E.H.

AU - Manulis-Sasson, S.

PY - 2013

Y1 - 2013

N2 - Dickeya strains isolated in Israel in 2006–2010 were characterized by dnaX sequence analysis, pulsed-field gel electrophoresis (PFGE), biochemical assays and pectolytic activity, and found to be homogeneous: most of them could be classified as ‘Dickeya solani’. Of the 34 strains isolated from imported seed tubers or potato plants grown from imported seed, 32 were typed as ‘D. solani’ and only two were characterized as Dickeya dianthicola. Biovar typing indicated that all ‘D. solani’ strains were biovar 3. ‘Dickeya solani’ strains were most closely related to Dickeya dadantii subsp. dieffenbachiae according to PFGE and dnaX analyses and both species exhibited high pectolytic activity. Expression levels of two putative virulence genes, pelL (encoding a pectic enzyme) and dspE (encoding a type III effector) were significantly induced in ‘D. solani’ strains isolated from potato plants or tubers grown in hot climates such as the Negev region in Israel, compared to those isolated from seed tubers imported from the Netherlands, France or Germany. Results of this study support the hypothesis that ‘D. solani’ strains isolated in Israel are also clonal; however, they appear to be more virulent than strains isolated in Europe

AB - Dickeya strains isolated in Israel in 2006–2010 were characterized by dnaX sequence analysis, pulsed-field gel electrophoresis (PFGE), biochemical assays and pectolytic activity, and found to be homogeneous: most of them could be classified as ‘Dickeya solani’. Of the 34 strains isolated from imported seed tubers or potato plants grown from imported seed, 32 were typed as ‘D. solani’ and only two were characterized as Dickeya dianthicola. Biovar typing indicated that all ‘D. solani’ strains were biovar 3. ‘Dickeya solani’ strains were most closely related to Dickeya dadantii subsp. dieffenbachiae according to PFGE and dnaX analyses and both species exhibited high pectolytic activity. Expression levels of two putative virulence genes, pelL (encoding a pectic enzyme) and dspE (encoding a type III effector) were significantly induced in ‘D. solani’ strains isolated from potato plants or tubers grown in hot climates such as the Negev region in Israel, compared to those isolated from seed tubers imported from the Netherlands, France or Germany. Results of this study support the hypothesis that ‘D. solani’ strains isolated in Israel are also clonal; however, they appear to be more virulent than strains isolated in Europe

KW - soft-rot erwinias

KW - chrysanthemi

KW - population

KW - israel

KW - crops

KW - pcr

U2 - 10.1111/ppa.12030

DO - 10.1111/ppa.12030

M3 - Article

SN - 0032-0862

VL - 62

SP - 1097

EP - 1105

JO - Plant Pathology

JF - Plant Pathology

IS - 5

ER -

Characterization of Dickeya strains isolated from potato grown under hot-climate conditions

Abstract

Keywords

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