Characterization of chromosomal regions containing quantitative trait loci determining specificity towards bread and durum wheat cultivars in the fungal wheat pathogen Mycosphaerella graminicola

S.B. Ware; A.H.J. Wittenberg; E.C.P. Verstappen; T.A.J. van der Lee; H.J. Schouten; S.B. Goodwin; J. Grimwood; J. Bristow; I. Grigoriev; G.H.J. Kema

Abstract

Specificity of the haploid, plant-pathogenic fungus Mycosphaerella graminicola towards durum (tetraploid, genomes AABB) versus bread (AABBDD) wheat is well documented. To estimate the number of genes involved in host species specificity, we crossed an isolate of M. graminicola collected from bread wheat (IPO323) with one from durum wheat (IPO95052) and analyzed the pathogenicity of 163 progeny to four cultivars of bread wheat and three of durum. Segregation data for pathogenicity to the seven cultivars of wheat were added to a high-density genetic linkage map containing 1,144 Diversity Array Technology (DArT) markers. Combined analyses of the phenotypic data and the DArT map identified nine quantitative trait loci (QTL) for cultivar specificity on seven linkage groups. These genetic loci were aligned to the whole-genome sequence of M. graminicola to identify the genes involved in specificity. To our knowledge, this is the first comprehensive QTL analysis of virulence performed on a plant-pathogenic fungus. The previously accepted hypothesis that host species specificity among isolates of M. graminicola is under controle of a single gene appears to be incorrect. Instead, specificity of M. graminicola to durum versus bread wheat appears to be under multigenic control. Therefore, pathogenicity to different species of cultivated wheat most likely is conditioned by a mix of genetically inherited factors, and the distinction between host species and cultivar specificity is not clearly defined

Original language	English
Title of host publication	Proceedings of the 15th Plant and Animal Genome Conference, San Diego, California, USA, 13-17 January 2007
Pages	W360
Publication status	Published - 2007
Event	15th Plant and Animal Genome Conference, San Diego, California, USA - Duration: 13 Jan 2007 → 17 Jan 2007

Conference/symposium

Conference/symposium	15th Plant and Animal Genome Conference, San Diego, California, USA
Period	13/01/07 → 17/01/07

Cite this

Ware, S. B., Wittenberg, A. H. J., Verstappen, E. C. P., van der Lee, T. A. J., Schouten, H. J., Goodwin, S. B., Grimwood, J., Bristow, J., Grigoriev, I., & Kema, G. H. J. (2007). Characterization of chromosomal regions containing quantitative trait loci determining specificity towards bread and durum wheat cultivars in the fungal wheat pathogen Mycosphaerella graminicola. In Proceedings of the 15th Plant and Animal Genome Conference, San Diego, California, USA, 13-17 January 2007 (pp. W360)

Ware, S.B. ; Wittenberg, A.H.J. ; Verstappen, E.C.P. et al. / Characterization of chromosomal regions containing quantitative trait loci determining specificity towards bread and durum wheat cultivars in the fungal wheat pathogen Mycosphaerella graminicola. Proceedings of the 15th Plant and Animal Genome Conference, San Diego, California, USA, 13-17 January 2007. 2007. pp. W360

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title = "Characterization of chromosomal regions containing quantitative trait loci determining specificity towards bread and durum wheat cultivars in the fungal wheat pathogen Mycosphaerella graminicola",

abstract = "Specificity of the haploid, plant-pathogenic fungus Mycosphaerella graminicola towards durum (tetraploid, genomes AABB) versus bread (AABBDD) wheat is well documented. To estimate the number of genes involved in host species specificity, we crossed an isolate of M. graminicola collected from bread wheat (IPO323) with one from durum wheat (IPO95052) and analyzed the pathogenicity of 163 progeny to four cultivars of bread wheat and three of durum. Segregation data for pathogenicity to the seven cultivars of wheat were added to a high-density genetic linkage map containing 1,144 Diversity Array Technology (DArT) markers. Combined analyses of the phenotypic data and the DArT map identified nine quantitative trait loci (QTL) for cultivar specificity on seven linkage groups. These genetic loci were aligned to the whole-genome sequence of M. graminicola to identify the genes involved in specificity. To our knowledge, this is the first comprehensive QTL analysis of virulence performed on a plant-pathogenic fungus. The previously accepted hypothesis that host species specificity among isolates of M. graminicola is under controle of a single gene appears to be incorrect. Instead, specificity of M. graminicola to durum versus bread wheat appears to be under multigenic control. Therefore, pathogenicity to different species of cultivated wheat most likely is conditioned by a mix of genetically inherited factors, and the distinction between host species and cultivar specificity is not clearly defined",

author = "S.B. Ware and A.H.J. Wittenberg and E.C.P. Verstappen and {van der Lee}, T.A.J. and H.J. Schouten and S.B. Goodwin and J. Grimwood and J. Bristow and I. Grigoriev and G.H.J. Kema",

note = "http://www.intl-pag.org/15/abstracts/PAG15_W56_360.html; 15th Plant and Animal Genome Conference, San Diego, California, USA ; Conference date: 13-01-2007 Through 17-01-2007",

year = "2007",

language = "English",

pages = "W360",

booktitle = "Proceedings of the 15th Plant and Animal Genome Conference, San Diego, California, USA, 13-17 January 2007",

}

Ware, SB, Wittenberg, AHJ, Verstappen, ECP , van der Lee, TAJ , Schouten, HJ, Goodwin, SB, Grimwood, J, Bristow, J, Grigoriev, I & Kema, GHJ 2007, Characterization of chromosomal regions containing quantitative trait loci determining specificity towards bread and durum wheat cultivars in the fungal wheat pathogen Mycosphaerella graminicola. in Proceedings of the 15th Plant and Animal Genome Conference, San Diego, California, USA, 13-17 January 2007. pp. W360, 15th Plant and Animal Genome Conference, San Diego, California, USA, 13/01/07.

Characterization of chromosomal regions containing quantitative trait loci determining specificity towards bread and durum wheat cultivars in the fungal wheat pathogen Mycosphaerella graminicola. / Ware, S.B.; Wittenberg, A.H.J.; Verstappen, E.C.P. et al.
Proceedings of the 15th Plant and Animal Genome Conference, San Diego, California, USA, 13-17 January 2007. 2007. p. W360.

Research output: Chapter in Book/Report/Conference proceeding › Abstract

TY - CHAP

T1 - Characterization of chromosomal regions containing quantitative trait loci determining specificity towards bread and durum wheat cultivars in the fungal wheat pathogen Mycosphaerella graminicola

AU - Ware, S.B.

AU - Wittenberg, A.H.J.

AU - Verstappen, E.C.P.

AU - van der Lee, T.A.J.

AU - Schouten, H.J.

AU - Goodwin, S.B.

AU - Grimwood, J.

AU - Bristow, J.

AU - Grigoriev, I.

AU - Kema, G.H.J.

N1 - http://www.intl-pag.org/15/abstracts/PAG15_W56_360.html

PY - 2007

Y1 - 2007

N2 - Specificity of the haploid, plant-pathogenic fungus Mycosphaerella graminicola towards durum (tetraploid, genomes AABB) versus bread (AABBDD) wheat is well documented. To estimate the number of genes involved in host species specificity, we crossed an isolate of M. graminicola collected from bread wheat (IPO323) with one from durum wheat (IPO95052) and analyzed the pathogenicity of 163 progeny to four cultivars of bread wheat and three of durum. Segregation data for pathogenicity to the seven cultivars of wheat were added to a high-density genetic linkage map containing 1,144 Diversity Array Technology (DArT) markers. Combined analyses of the phenotypic data and the DArT map identified nine quantitative trait loci (QTL) for cultivar specificity on seven linkage groups. These genetic loci were aligned to the whole-genome sequence of M. graminicola to identify the genes involved in specificity. To our knowledge, this is the first comprehensive QTL analysis of virulence performed on a plant-pathogenic fungus. The previously accepted hypothesis that host species specificity among isolates of M. graminicola is under controle of a single gene appears to be incorrect. Instead, specificity of M. graminicola to durum versus bread wheat appears to be under multigenic control. Therefore, pathogenicity to different species of cultivated wheat most likely is conditioned by a mix of genetically inherited factors, and the distinction between host species and cultivar specificity is not clearly defined

AB - Specificity of the haploid, plant-pathogenic fungus Mycosphaerella graminicola towards durum (tetraploid, genomes AABB) versus bread (AABBDD) wheat is well documented. To estimate the number of genes involved in host species specificity, we crossed an isolate of M. graminicola collected from bread wheat (IPO323) with one from durum wheat (IPO95052) and analyzed the pathogenicity of 163 progeny to four cultivars of bread wheat and three of durum. Segregation data for pathogenicity to the seven cultivars of wheat were added to a high-density genetic linkage map containing 1,144 Diversity Array Technology (DArT) markers. Combined analyses of the phenotypic data and the DArT map identified nine quantitative trait loci (QTL) for cultivar specificity on seven linkage groups. These genetic loci were aligned to the whole-genome sequence of M. graminicola to identify the genes involved in specificity. To our knowledge, this is the first comprehensive QTL analysis of virulence performed on a plant-pathogenic fungus. The previously accepted hypothesis that host species specificity among isolates of M. graminicola is under controle of a single gene appears to be incorrect. Instead, specificity of M. graminicola to durum versus bread wheat appears to be under multigenic control. Therefore, pathogenicity to different species of cultivated wheat most likely is conditioned by a mix of genetically inherited factors, and the distinction between host species and cultivar specificity is not clearly defined

M3 - Abstract

SP - W360

BT - Proceedings of the 15th Plant and Animal Genome Conference, San Diego, California, USA, 13-17 January 2007

T2 - 15th Plant and Animal Genome Conference, San Diego, California, USA

Y2 - 13 January 2007 through 17 January 2007

ER -

Ware SB, Wittenberg AHJ, Verstappen ECP , van der Lee TAJ , Schouten HJ, Goodwin SB et al. Characterization of chromosomal regions containing quantitative trait loci determining specificity towards bread and durum wheat cultivars in the fungal wheat pathogen Mycosphaerella graminicola. In Proceedings of the 15th Plant and Animal Genome Conference, San Diego, California, USA, 13-17 January 2007. 2007. p. W360

Characterization of chromosomal regions containing quantitative trait loci determining specificity towards bread and durum wheat cultivars in the fungal wheat pathogen Mycosphaerella graminicola

Abstract

Conference/symposium

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