Characterization of chromosomal regions containing quantitative trait loci determining specificity towards bread and durum wheat cultivars in the fungal wheat pathogen Mycosphaerella graminicola

S.B. Ware, A.H.J. Wittenberg, E.C.P. Verstappen, T.A.J. van der Lee, H.J. Schouten, S.B. Goodwin, J. Grimwood, J. Bristow, I. Grigoriev, G.H.J. Kema

    Research output: Chapter in Book/Report/Conference proceedingAbstract

    Abstract

    Specificity of the haploid, plant-pathogenic fungus Mycosphaerella graminicola towards durum (tetraploid, genomes AABB) versus bread (AABBDD) wheat is well documented. To estimate the number of genes involved in host species specificity, we crossed an isolate of M. graminicola collected from bread wheat (IPO323) with one from durum wheat (IPO95052) and analyzed the pathogenicity of 163 progeny to four cultivars of bread wheat and three of durum. Segregation data for pathogenicity to the seven cultivars of wheat were added to a high-density genetic linkage map containing 1,144 Diversity Array Technology (DArT) markers. Combined analyses of the phenotypic data and the DArT map identified nine quantitative trait loci (QTL) for cultivar specificity on seven linkage groups. These genetic loci were aligned to the whole-genome sequence of M. graminicola to identify the genes involved in specificity. To our knowledge, this is the first comprehensive QTL analysis of virulence performed on a plant-pathogenic fungus. The previously accepted hypothesis that host species specificity among isolates of M. graminicola is under controle of a single gene appears to be incorrect. Instead, specificity of M. graminicola to durum versus bread wheat appears to be under multigenic control. Therefore, pathogenicity to different species of cultivated wheat most likely is conditioned by a mix of genetically inherited factors, and the distinction between host species and cultivar specificity is not clearly defined
    Original languageEnglish
    Title of host publicationProceedings of the 15th Plant and Animal Genome Conference, San Diego, California, USA, 13-17 January 2007
    PagesW360
    Publication statusPublished - 2007
    Event15th Plant and Animal Genome Conference, San Diego, California, USA -
    Duration: 13 Jan 200717 Jan 2007

    Conference

    Conference15th Plant and Animal Genome Conference, San Diego, California, USA
    Period13/01/0717/01/07

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