Characterization of a spore-specific protein of the Bacillus cereus group

C. From, M. van der Voort, T. Abee, P.E. Granum

Research output: Contribution to journalLetterAcademicpeer-review

1 Citation (Scopus)

Abstract

Bc1245 is a monocistronic chromosomal gene of Bacillus cereus ATCC 14579 encoding a putative protein of 143 amino acids identified in this study to have a spore-related function in B. cereus. Bc1245 is highly conserved in the genome of members of the B. cereus group, indicating an important function of the gene in this group of bacteria. Quantitative PCR revealed that bc1245 is transcribed late in sporulation (upon formation of phase-bright spores) and at the same time as the mother cell–specific transcription factor sK. The sK regulon includes structural components of the spore (such as coat proteins), and it is therefore plausible that bc1245 might encode a structural outer spore protein. This was confirmed by detection of BC1245 in exosporium extracts from B. cereus by immunoblotting against BC1245 antiserum.
Original languageEnglish
Pages (from-to)152-159
JournalFEMS Microbiology Letters
Volume331
Issue number2
DOIs
Publication statusPublished - 2012

Fingerprint

Bacillus cereus
Spores
Proteins
Regulon
Capsid Proteins
Immunoblotting
Genes
Immune Sera
Transcription Factors
Genome
Bacteria
Amino Acids
Polymerase Chain Reaction

Keywords

  • gram-positive bacteria
  • low-gc-content
  • anthracis exosporium
  • nucleotide-sequence
  • sigma-factors
  • subtilis
  • surface
  • outer
  • tonb
  • germination

Cite this

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title = "Characterization of a spore-specific protein of the Bacillus cereus group",
abstract = "Bc1245 is a monocistronic chromosomal gene of Bacillus cereus ATCC 14579 encoding a putative protein of 143 amino acids identified in this study to have a spore-related function in B. cereus. Bc1245 is highly conserved in the genome of members of the B. cereus group, indicating an important function of the gene in this group of bacteria. Quantitative PCR revealed that bc1245 is transcribed late in sporulation (upon formation of phase-bright spores) and at the same time as the mother cell–specific transcription factor sK. The sK regulon includes structural components of the spore (such as coat proteins), and it is therefore plausible that bc1245 might encode a structural outer spore protein. This was confirmed by detection of BC1245 in exosporium extracts from B. cereus by immunoblotting against BC1245 antiserum.",
keywords = "gram-positive bacteria, low-gc-content, anthracis exosporium, nucleotide-sequence, sigma-factors, subtilis, surface, outer, tonb, germination",
author = "C. From and {van der Voort}, M. and T. Abee and P.E. Granum",
year = "2012",
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language = "English",
volume = "331",
pages = "152--159",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Oxford University Press",
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Characterization of a spore-specific protein of the Bacillus cereus group. / From, C.; van der Voort, M.; Abee, T.; Granum, P.E.

In: FEMS Microbiology Letters, Vol. 331, No. 2, 2012, p. 152-159.

Research output: Contribution to journalLetterAcademicpeer-review

TY - JOUR

T1 - Characterization of a spore-specific protein of the Bacillus cereus group

AU - From, C.

AU - van der Voort, M.

AU - Abee, T.

AU - Granum, P.E.

PY - 2012

Y1 - 2012

N2 - Bc1245 is a monocistronic chromosomal gene of Bacillus cereus ATCC 14579 encoding a putative protein of 143 amino acids identified in this study to have a spore-related function in B. cereus. Bc1245 is highly conserved in the genome of members of the B. cereus group, indicating an important function of the gene in this group of bacteria. Quantitative PCR revealed that bc1245 is transcribed late in sporulation (upon formation of phase-bright spores) and at the same time as the mother cell–specific transcription factor sK. The sK regulon includes structural components of the spore (such as coat proteins), and it is therefore plausible that bc1245 might encode a structural outer spore protein. This was confirmed by detection of BC1245 in exosporium extracts from B. cereus by immunoblotting against BC1245 antiserum.

AB - Bc1245 is a monocistronic chromosomal gene of Bacillus cereus ATCC 14579 encoding a putative protein of 143 amino acids identified in this study to have a spore-related function in B. cereus. Bc1245 is highly conserved in the genome of members of the B. cereus group, indicating an important function of the gene in this group of bacteria. Quantitative PCR revealed that bc1245 is transcribed late in sporulation (upon formation of phase-bright spores) and at the same time as the mother cell–specific transcription factor sK. The sK regulon includes structural components of the spore (such as coat proteins), and it is therefore plausible that bc1245 might encode a structural outer spore protein. This was confirmed by detection of BC1245 in exosporium extracts from B. cereus by immunoblotting against BC1245 antiserum.

KW - gram-positive bacteria

KW - low-gc-content

KW - anthracis exosporium

KW - nucleotide-sequence

KW - sigma-factors

KW - subtilis

KW - surface

KW - outer

KW - tonb

KW - germination

U2 - 10.1111/j.1574-6968.2012.02562.x

DO - 10.1111/j.1574-6968.2012.02562.x

M3 - Letter

VL - 331

SP - 152

EP - 159

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -