TY - JOUR
T1 - Characterization of a nucleopolyhedrovirus isolated from the laboratory rearing of the beet armyworm Spodoptera Exigua (HBN.) in Poland
AU - Jakubowska, A.K.
AU - Vlak, J.M.
AU - Ziemnicka, J.
PY - 2005
Y1 - 2005
N2 - A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera
exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral
bioinsecticide virus Spod-XR, was characterized molecularly and biologically.
Phylogenetic analysis based on three conserved baculovirus genes, polh, lef-8 and
pif-2, showed the highest homology of SeMNPV (P) to Mamestra brassicae (Mb)
MNPV and M. configurata (Maco) MNPV, and much less to SeMNPV (Spod-XR).
These findings were confirmed by genomic DNA restriction profile analyses.
Bioassays revealed that SeMNPV isolated from the commercial bioinsecticide
Spod-XR was themost infectious for S. exigua, while the infectivity of SeMNPV (P)
and MbMNPV was significantly lower. These data suggest that SeMNPV (P) is a
variant of MbMNPV.
AB - A nucleopolyhedrovirus isolated from the beet armyworm Spodoptera
exigua (Polish laboratory culture), SeMNPV (P), morphologically similar to the viral
bioinsecticide virus Spod-XR, was characterized molecularly and biologically.
Phylogenetic analysis based on three conserved baculovirus genes, polh, lef-8 and
pif-2, showed the highest homology of SeMNPV (P) to Mamestra brassicae (Mb)
MNPV and M. configurata (Maco) MNPV, and much less to SeMNPV (Spod-XR).
These findings were confirmed by genomic DNA restriction profile analyses.
Bioassays revealed that SeMNPV isolated from the commercial bioinsecticide
Spod-XR was themost infectious for S. exigua, while the infectivity of SeMNPV (P)
and MbMNPV was significantly lower. These data suggest that SeMNPV (P) is a
variant of MbMNPV.
M3 - Article
SN - 1427-4345
VL - 45
SP - 279
EP - 286
JO - Journal of Plant Protection Research
JF - Journal of Plant Protection Research
IS - 4
ER -