Characterization of a microsecond intermediate in the laser flash Photolysis of small Phytochrome from oat

S.E. Braslavsky, J.I. Matthews, H.J. Herbert, J. de Kok, C.J.P. Spruit, K. Schaffner

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    46 Citations (Scopus)

    Abstract

    Irradiation of small phytochrome from oat in its Pr form with 15 ns laser pulses of different wavelengths(605–655 nm) gave rise to a difference absorption with maxima at 400 and 685 nm for the first detectable transient. Bleaching of a 660 nm band was observed, non-recuperable up to 1 ms. The transient absorption has a lifetime of 70±15 μs at 273 K. The transient is tentatively identified as lumi-R and the conformation of its chromophore is postulated to be more extended than that of Pr. A deviation from the exponential decay of the lumi-R absorption at 284 and 300 K and the lack of observable enhancement of the far-red absorption within 1 ms are interpreted in terms of the appearance of still other intermediates on this time scale between lumi-R and Pfr phytochrome.
    Original languageEnglish
    Pages (from-to)417-420
    JournalPhotochemistry and Photobiology
    Volume31
    DOIs
    Publication statusPublished - 1980

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