Characterization and Transcriptional Profile of Genes Involved in Glycoalkaloid Biosynthesis in New Varieties of Solanum tuberosum L.

Roberta Fogliatto Mariot, Luisa Abruzzi De Oliveira, M.M. Voorhuijzen, Martijn Staats, R.C.B. Hutten, J.P. Van Dijk, E.J. Kok, Jeverson Frazzon*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

5 Citations (Scopus)

Abstract

Before commercial release, new potato (Solanum tuberosum) varieties must be evaluated for content of toxic compounds such as glycoalkaloids (GAs), which are potent poisons. GA biosynthesis proceeds via the cholesterol pathway to α-chaconine and α-solanine. The goal of this study was to evaluate the relationship between total glycoalkaloid (TGA) content and the expression of GAME, SGT1, and SGT3 genes in potato tubers. TGA content was measured by HPLC-MS, and reverse transcription quantitative polymerase chain reactions were performed to determine the relative expression of GAME, SGT1, and SGT3 genes. We searched for cis-elements of the transcription start site using the PlantPAN database. There was a relationship between TGA content and the relative expression of GAME, SGT1, and SGT3 genes in potato tubers. Putative promoter regions showed the presence of several cis-elements related to biotic and abiotic stresses and light. These findings provide an important step toward understanding TGA regulation and variation in potato tubers.

Original languageEnglish
Pages (from-to)988-996
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number4
DOIs
Publication statusPublished - 2016

Keywords

  • GAMEs
  • gene expression
  • glycoalkaloids
  • SGT1 and SGT3 genes
  • Solanum tuberosum

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