Characterization and Transcriptional Profile of Genes Involved in Glycoalkaloid Biosynthesis in New Varieties of Solanum tuberosum L.

Roberta Fogliatto Mariot, Luisa Abruzzi De Oliveira, M.M. Voorhuijzen, Martijn Staats, R.C.B. Hutten, J.P. Van Dijk, E.J. Kok, Jeverson Frazzon*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Before commercial release, new potato (Solanum tuberosum) varieties must be evaluated for content of toxic compounds such as glycoalkaloids (GAs), which are potent poisons. GA biosynthesis proceeds via the cholesterol pathway to α-chaconine and α-solanine. The goal of this study was to evaluate the relationship between total glycoalkaloid (TGA) content and the expression of GAME, SGT1, and SGT3 genes in potato tubers. TGA content was measured by HPLC-MS, and reverse transcription quantitative polymerase chain reactions were performed to determine the relative expression of GAME, SGT1, and SGT3 genes. We searched for cis-elements of the transcription start site using the PlantPAN database. There was a relationship between TGA content and the relative expression of GAME, SGT1, and SGT3 genes in potato tubers. Putative promoter regions showed the presence of several cis-elements related to biotic and abiotic stresses and light. These findings provide an important step toward understanding TGA regulation and variation in potato tubers.

Original languageEnglish
Pages (from-to)988-996
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number4
DOIs
Publication statusPublished - 2016

Fingerprint

glycoalkaloids
Biosynthesis
new variety
Solanum tuberosum
Genes
Poisons
biosynthesis
Solanine
potatoes
tubers
genes
Transcription Initiation Site
Polymerase chain reaction
Transcription
Genetic Promoter Regions
Cholesterol
alpha-chaconine
solanine
Reverse Transcription
biotic stress

Keywords

  • GAMEs
  • gene expression
  • glycoalkaloids
  • SGT1 and SGT3 genes
  • Solanum tuberosum

Cite this

@article{6b19172a4302406c8fbec8d82449247a,
title = "Characterization and Transcriptional Profile of Genes Involved in Glycoalkaloid Biosynthesis in New Varieties of Solanum tuberosum L.",
abstract = "Before commercial release, new potato (Solanum tuberosum) varieties must be evaluated for content of toxic compounds such as glycoalkaloids (GAs), which are potent poisons. GA biosynthesis proceeds via the cholesterol pathway to α-chaconine and α-solanine. The goal of this study was to evaluate the relationship between total glycoalkaloid (TGA) content and the expression of GAME, SGT1, and SGT3 genes in potato tubers. TGA content was measured by HPLC-MS, and reverse transcription quantitative polymerase chain reactions were performed to determine the relative expression of GAME, SGT1, and SGT3 genes. We searched for cis-elements of the transcription start site using the PlantPAN database. There was a relationship between TGA content and the relative expression of GAME, SGT1, and SGT3 genes in potato tubers. Putative promoter regions showed the presence of several cis-elements related to biotic and abiotic stresses and light. These findings provide an important step toward understanding TGA regulation and variation in potato tubers.",
keywords = "GAMEs, gene expression, glycoalkaloids, SGT1 and SGT3 genes, Solanum tuberosum",
author = "Mariot, {Roberta Fogliatto} and {De Oliveira}, {Luisa Abruzzi} and M.M. Voorhuijzen and Martijn Staats and R.C.B. Hutten and {Van Dijk}, J.P. and E.J. Kok and Jeverson Frazzon",
year = "2016",
doi = "10.1021/acs.jafc.5b05519",
language = "English",
volume = "64",
pages = "988--996",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
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}

Characterization and Transcriptional Profile of Genes Involved in Glycoalkaloid Biosynthesis in New Varieties of Solanum tuberosum L. / Mariot, Roberta Fogliatto; De Oliveira, Luisa Abruzzi; Voorhuijzen, M.M.; Staats, Martijn; Hutten, R.C.B.; Van Dijk, J.P.; Kok, E.J.; Frazzon, Jeverson.

In: Journal of Agricultural and Food Chemistry, Vol. 64, No. 4, 2016, p. 988-996.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Characterization and Transcriptional Profile of Genes Involved in Glycoalkaloid Biosynthesis in New Varieties of Solanum tuberosum L.

AU - Mariot, Roberta Fogliatto

AU - De Oliveira, Luisa Abruzzi

AU - Voorhuijzen, M.M.

AU - Staats, Martijn

AU - Hutten, R.C.B.

AU - Van Dijk, J.P.

AU - Kok, E.J.

AU - Frazzon, Jeverson

PY - 2016

Y1 - 2016

N2 - Before commercial release, new potato (Solanum tuberosum) varieties must be evaluated for content of toxic compounds such as glycoalkaloids (GAs), which are potent poisons. GA biosynthesis proceeds via the cholesterol pathway to α-chaconine and α-solanine. The goal of this study was to evaluate the relationship between total glycoalkaloid (TGA) content and the expression of GAME, SGT1, and SGT3 genes in potato tubers. TGA content was measured by HPLC-MS, and reverse transcription quantitative polymerase chain reactions were performed to determine the relative expression of GAME, SGT1, and SGT3 genes. We searched for cis-elements of the transcription start site using the PlantPAN database. There was a relationship between TGA content and the relative expression of GAME, SGT1, and SGT3 genes in potato tubers. Putative promoter regions showed the presence of several cis-elements related to biotic and abiotic stresses and light. These findings provide an important step toward understanding TGA regulation and variation in potato tubers.

AB - Before commercial release, new potato (Solanum tuberosum) varieties must be evaluated for content of toxic compounds such as glycoalkaloids (GAs), which are potent poisons. GA biosynthesis proceeds via the cholesterol pathway to α-chaconine and α-solanine. The goal of this study was to evaluate the relationship between total glycoalkaloid (TGA) content and the expression of GAME, SGT1, and SGT3 genes in potato tubers. TGA content was measured by HPLC-MS, and reverse transcription quantitative polymerase chain reactions were performed to determine the relative expression of GAME, SGT1, and SGT3 genes. We searched for cis-elements of the transcription start site using the PlantPAN database. There was a relationship between TGA content and the relative expression of GAME, SGT1, and SGT3 genes in potato tubers. Putative promoter regions showed the presence of several cis-elements related to biotic and abiotic stresses and light. These findings provide an important step toward understanding TGA regulation and variation in potato tubers.

KW - GAMEs

KW - gene expression

KW - glycoalkaloids

KW - SGT1 and SGT3 genes

KW - Solanum tuberosum

U2 - 10.1021/acs.jafc.5b05519

DO - 10.1021/acs.jafc.5b05519

M3 - Article

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SP - 988

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JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

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ER -