Characterisation of pectin-xylan complexes in tomato primary plant cell walls

Suzanne E. Broxterman, Henk A. Schols*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

The primary plant cell wall is composed of a complex network of pectin, hemicellulose and cellulose. Potential interactions between these polysaccharides were studied for carrot, tomato and strawberry, with a focus on the role of pectin. The Chelating agent Unextractable Solids (ChUS), the residue after water- and EDTA extraction, was ball milled and subsequently water extracted. For tomato and strawberry, pectin and substantial amounts of hemicellulose were solubilised. Anion exchange chromatography (AEC) showed co-elution of pectin and acetylated glucuronoxylan in tomato, representing 18% of solubilised uronic acid and 48% of solubilised xylose by ball milling from ChUS. The existence of a covalently linked pectin-xylan complex was proposed since xylan co-precipitated with pectin under mild alkali conditions. It was proposed that pectin links with xylan through the RG-I region since degradation of HG did not alter AEC elution patterns for RG-I and xylan, suggesting RG-I – xylan interactions.
Original languageEnglish
Pages (from-to)269-276
JournalCarbohydrate Polymers
Volume197
DOIs
Publication statusPublished - Oct 2018

Keywords

  • Carrot
  • Cross-link
  • Planetary ball milling
  • Primary plant cell wall
  • Strawberry
  • Tomato

Fingerprint Dive into the research topics of 'Characterisation of pectin-xylan complexes in tomato primary plant cell walls'. Together they form a unique fingerprint.

Cite this