Characterisation of antioxidant and antiproliferative acidic polysaccharides from Chinese wolfberry fruits

N.W. He, X.B. Yang, Y.D. Jiao, L. Tian, Y. Zhao

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93 Citations (Scopus)

Abstract

Wolfberry fruit polysaccharides (WFPs) were isolated by hot-water extraction and ethanol precipitation. With HPLC analysis, WFPs were for the first time identified as acidic polysaccharides with galacturonic acid being the main component monosaccharide (24.9%), followed by galactose (21.3%), arabinose (18.5%), and glucose (15.9%), accounting for up to 80.6% of the molar percentage. WFPs exhibited a high antioxidant activity and a dose-dependent antiproliferative effect, with IC50 values of 134.9, 70.1 and 138.4 mu g/mL against A549, MCF-7 and LoVo cancer cells after 48 h of incubation as estimated by MTT assay, respectively. Flow cytometry analysis showed that WFPs exerted a stimulatory effect on apoptosis of MCF-7 cells, and induced the cell-cycle arrest at the G0/G1 phase, with the observation of intracellular ROS production and DNA damage. The present study demonstrated that these polysaccharides might have the potential to provide significant natural defence against human cancer.
Original languageEnglish
Pages (from-to)978-989
JournalFood Chemistry
Volume133
Issue number3
DOIs
Publication statusPublished - 2012

Keywords

  • lycium-barbarum polysaccharides
  • breast-cancer cells
  • in-vitro
  • apoptosis
  • extraction
  • arrest
  • growth
  • anticancer
  • prevention
  • cranberry

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