Characterisation of an acetylcholine receptor gene of Haemonchus contortus in relation to levamisole resistance

R. Hoekstra, A. Visser, L. Wiley, A.S. Weiss, N.C. Sangster, M.H. Roos

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    45 Citations (Scopus)

    Abstract

    The anthelmintic drug levamisole is thought to bind to nicotinic acetylcholine receptors of nematodes. It is possible that resistance to this drug is associated with either a change in binding characteristics or a reduction in the number of nicotinic acetylcholine receptors. Therefore, the molecular mechanism of levamisole resistance in the parasitic nematode Haemonchus contortus was studied by isolating and characterising cDNA clones encoding a putative ligand binding nicotinic acetylcholine receptor subunit, HCA1, of two susceptible and one levamisole resistant population. HcA1 is related to unc-38, a nicotinic acetylcholine receptor subunit gene associated with levamisole resistance in Caenorhabditis elegans. Although extensive sequence analyses of hca1 sequences revealed poly-morphism at amino acid level, no association with levamisole resistance could be detected. Restriction fragment length polymorphism analyses confirmed that, although polymorphism was detected, no selection of a specific allele of heal has taken place during selection for levamisole resistance in various levamisole resistant populations.
    Original languageEnglish
    Pages (from-to)179-187
    JournalMolecular and biochemical parasitology
    Volume84
    Issue number2
    DOIs
    Publication statusPublished - 1997

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