Chaperones of the endoplasmic reticulum are required for Ve1-mediated resistance to Verticillium

T.W.H. Liebrand, A. Kombrink, Z. Zhang, J. Sklenar, A.M.E. Jones, S. Robatzek, B.P.H.J. Thomma, M.H.A.J. Joosten

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

The tomato receptor-like protein (RLP) Ve1 mediates resistance to the vascular fungal pathogen Verticillium dahliae. To identify the proteins required for Ve1 function, we transiently expressed and immunopurified functional Ve1-enhanced green fluorescent protein (eGFP) from Nicotiana benthamiana leaves, followed by mass spectrometry. This resulted in the identification of peptides originating from the endoplasmic reticulum (ER)-resident chaperones HSP70 binding proteins (BiPs) and a lectin-type calreticulin (CRT). Knock-down of the different BiPs and CRTs in tomato resulted in compromised Ve1-mediated resistance to V.dahliae in most cases, showing that these chaperones play an important role in Ve1 functionality. Recently, it has been shown that one particular CRT is required for the biogenesis of the RLP-type Cladosporium fulvum resistance protein Cf-4 of tomato, as silencing of CRT3a resulted in a reduced pool of complex glycosylated Cf-4 protein. In contrast, knock-down of the various CRTs in N.benthamiana or N.tabacum did not result in reduced accumulation of mature complex glycosylated Ve1 protein. Together, this study shows that the BiP and CRT ER chaperones differentially contribute to Cf-4- and Ve1-mediated immunity.
Original languageEnglish
Pages (from-to)109-117
JournalMolecular Plant Pathology
Volume15
Issue number1
DOIs
Publication statusPublished - 2014

Fingerprint

Verticillium
Endoplasmic Reticulum
endoplasmic reticulum
calreticulin
Calreticulin
protein
Lycopersicon esculentum
Proteins
proteins
tomatoes
binding proteins
Carrier Proteins
Passalora fulva
Cladosporium
receptors
Verticillium dahliae
Nicotiana benthamiana
green fluorescent protein
Lectins
blood vessels

Keywords

  • receptor-like proteins
  • defective brassinosteroid receptor
  • pattern-recognition receptors
  • er quality-control
  • plant innate immunity
  • cell-surface
  • tomato ve1
  • arabidopsis
  • gene
  • perception

Cite this

Liebrand, T.W.H. ; Kombrink, A. ; Zhang, Z. ; Sklenar, J. ; Jones, A.M.E. ; Robatzek, S. ; Thomma, B.P.H.J. ; Joosten, M.H.A.J. / Chaperones of the endoplasmic reticulum are required for Ve1-mediated resistance to Verticillium. In: Molecular Plant Pathology. 2014 ; Vol. 15, No. 1. pp. 109-117.
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abstract = "The tomato receptor-like protein (RLP) Ve1 mediates resistance to the vascular fungal pathogen Verticillium dahliae. To identify the proteins required for Ve1 function, we transiently expressed and immunopurified functional Ve1-enhanced green fluorescent protein (eGFP) from Nicotiana benthamiana leaves, followed by mass spectrometry. This resulted in the identification of peptides originating from the endoplasmic reticulum (ER)-resident chaperones HSP70 binding proteins (BiPs) and a lectin-type calreticulin (CRT). Knock-down of the different BiPs and CRTs in tomato resulted in compromised Ve1-mediated resistance to V.dahliae in most cases, showing that these chaperones play an important role in Ve1 functionality. Recently, it has been shown that one particular CRT is required for the biogenesis of the RLP-type Cladosporium fulvum resistance protein Cf-4 of tomato, as silencing of CRT3a resulted in a reduced pool of complex glycosylated Cf-4 protein. In contrast, knock-down of the various CRTs in N.benthamiana or N.tabacum did not result in reduced accumulation of mature complex glycosylated Ve1 protein. Together, this study shows that the BiP and CRT ER chaperones differentially contribute to Cf-4- and Ve1-mediated immunity.",
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Chaperones of the endoplasmic reticulum are required for Ve1-mediated resistance to Verticillium. / Liebrand, T.W.H.; Kombrink, A.; Zhang, Z.; Sklenar, J.; Jones, A.M.E.; Robatzek, S.; Thomma, B.P.H.J.; Joosten, M.H.A.J.

In: Molecular Plant Pathology, Vol. 15, No. 1, 2014, p. 109-117.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Chaperones of the endoplasmic reticulum are required for Ve1-mediated resistance to Verticillium

AU - Liebrand, T.W.H.

AU - Kombrink, A.

AU - Zhang, Z.

AU - Sklenar, J.

AU - Jones, A.M.E.

AU - Robatzek, S.

AU - Thomma, B.P.H.J.

AU - Joosten, M.H.A.J.

PY - 2014

Y1 - 2014

N2 - The tomato receptor-like protein (RLP) Ve1 mediates resistance to the vascular fungal pathogen Verticillium dahliae. To identify the proteins required for Ve1 function, we transiently expressed and immunopurified functional Ve1-enhanced green fluorescent protein (eGFP) from Nicotiana benthamiana leaves, followed by mass spectrometry. This resulted in the identification of peptides originating from the endoplasmic reticulum (ER)-resident chaperones HSP70 binding proteins (BiPs) and a lectin-type calreticulin (CRT). Knock-down of the different BiPs and CRTs in tomato resulted in compromised Ve1-mediated resistance to V.dahliae in most cases, showing that these chaperones play an important role in Ve1 functionality. Recently, it has been shown that one particular CRT is required for the biogenesis of the RLP-type Cladosporium fulvum resistance protein Cf-4 of tomato, as silencing of CRT3a resulted in a reduced pool of complex glycosylated Cf-4 protein. In contrast, knock-down of the various CRTs in N.benthamiana or N.tabacum did not result in reduced accumulation of mature complex glycosylated Ve1 protein. Together, this study shows that the BiP and CRT ER chaperones differentially contribute to Cf-4- and Ve1-mediated immunity.

AB - The tomato receptor-like protein (RLP) Ve1 mediates resistance to the vascular fungal pathogen Verticillium dahliae. To identify the proteins required for Ve1 function, we transiently expressed and immunopurified functional Ve1-enhanced green fluorescent protein (eGFP) from Nicotiana benthamiana leaves, followed by mass spectrometry. This resulted in the identification of peptides originating from the endoplasmic reticulum (ER)-resident chaperones HSP70 binding proteins (BiPs) and a lectin-type calreticulin (CRT). Knock-down of the different BiPs and CRTs in tomato resulted in compromised Ve1-mediated resistance to V.dahliae in most cases, showing that these chaperones play an important role in Ve1 functionality. Recently, it has been shown that one particular CRT is required for the biogenesis of the RLP-type Cladosporium fulvum resistance protein Cf-4 of tomato, as silencing of CRT3a resulted in a reduced pool of complex glycosylated Cf-4 protein. In contrast, knock-down of the various CRTs in N.benthamiana or N.tabacum did not result in reduced accumulation of mature complex glycosylated Ve1 protein. Together, this study shows that the BiP and CRT ER chaperones differentially contribute to Cf-4- and Ve1-mediated immunity.

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KW - pattern-recognition receptors

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KW - plant innate immunity

KW - cell-surface

KW - tomato ve1

KW - arabidopsis

KW - gene

KW - perception

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JO - Molecular Plant Pathology

JF - Molecular Plant Pathology

SN - 1464-6722

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ER -