Changes in the repertoire of bovine milk proteins during mammary involution.

I. Boggs, B. Hine, G. Smolenski, K.A. Hettinga, L. Zhang, T. Wheeler

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Changes of abundance that occur in the repertoire of low abundance milk proteins after cessation of milk removal have not been characterised. Skimmed milk and whey from cows sampled at day 0 and either day 3 or day 8 after drying off were subjected to three untargeted proteomics techniques; 2-D gel electrophoresis, GeLC-MS, and dimethyl isotopic labelling of tryptic peptides. The changes observed included 45 fragments of abundant milk proteins and 36 host-defence proteins, suggesting activation of proteolysis and inflammation. The findings form a basis for adding value to dairy production.
LanguageEnglish
Pages65-75
JournalEuPA Open Proteomics
Volume9
DOIs
Publication statusPublished - 2015

Fingerprint

Milk Proteins
Milk
Breast
Proteolysis
Dairies
Electrophoresis, Gel, Two-Dimensional
Electrophoresis
Proteomics
Labeling
Drying
Gels
Chemical activation
Inflammation
Peptides
Proteins
Whey

Cite this

Boggs, I. ; Hine, B. ; Smolenski, G. ; Hettinga, K.A. ; Zhang, L. ; Wheeler, T. / Changes in the repertoire of bovine milk proteins during mammary involution. In: EuPA Open Proteomics. 2015 ; Vol. 9. pp. 65-75.
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Changes in the repertoire of bovine milk proteins during mammary involution. / Boggs, I.; Hine, B.; Smolenski, G.; Hettinga, K.A.; Zhang, L.; Wheeler, T.

In: EuPA Open Proteomics, Vol. 9, 2015, p. 65-75.

Research output: Contribution to journalArticleAcademicpeer-review

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AU - Zhang, L.

AU - Wheeler, T.

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