Changes in the diversity of pig ileal lactobacilli around weaning determined by means of 16S rRNA gene amplification and denaturing gradient gel electrophoresis

P. Janzcyk, R. Pieper, H. Smidt, W.B. Souffrant

Research output: Contribution to journalArticleAcademicpeer-review

61 Citations (Scopus)

Abstract

Our study aimed to provide a comprehensive characterization of changes in porcine intestinal Lactobacillus populations around the time of weaning based on 16S rRNA gene amplification and denaturing gradient gel electrophoresis (DGGE). DNA was extracted from the ileal contents of piglets at weaning (28 days of age) and after 1, 2, 5 and 11 days. PCR amplicons (V2-V3 fragments of 16S rRNA genes) were separated using DGGE. Predominant bands were excised and sequenced after reamplification. A band corresponding to Lactobacillus salivarius was present 1 and 2 days post-weaning (pw), while Lactobacillus crispatus was detected only 1 and 11 days pw. Lactobacillus sobrius gave the most dominant band in all animals. The number of bands decreased from 13±3 at weaning to 9±1 at 5 days pw, but the species richness had recovered by 11 days pw. The similarity of profiles between sampling days was high for 1 and 2 days pw (>91%), but was low for 5 and 11 days pw (
Original languageEnglish
Pages (from-to)132-140
JournalFEMS microbiology ecology
Volume61
Issue number1
DOIs
Publication statusPublished - 2007

Keywords

  • lactic-acid bacteria
  • molecular ecological analysis
  • human fecal samples
  • intestinal microbiota
  • caco-2 cells
  • piglets
  • pcr
  • dietary
  • dna
  • quantification

Fingerprint Dive into the research topics of 'Changes in the diversity of pig ileal lactobacilli around weaning determined by means of 16S rRNA gene amplification and denaturing gradient gel electrophoresis'. Together they form a unique fingerprint.

  • Cite this