Changes in milk proteome and metabolome associated with dry period length, energy balance and lactation stage in post parturient dairy cows

J. Lu, E.C. Antunes Fernandes, A.E. Páez Cano, J. Vinitwatanakhun, S. Boeren, A.C.M. van Hooijdonk, A.T.M. van Knegsel, J. Vervoort, K.A. Hettinga

Research output: Contribution to journalArticleAcademicpeer-review

42 Citations (Scopus)

Abstract

The early lactation period of dairy cows, which produce high quantities of milk, is normally characterized by an insufficient energy intake to cover milk production and maintenance requirements. Mobilization of body reserves occurs to compensate this negative energy balance (NEB), and probably as a consequence there is a higher susceptibility to diseases and metabolic disorders. There are several diagnostic methods to detect NEB, usually involving ketosis related parameters. Due to the easy availability of milk this is a preferred matrix, but simple and robust predictors of NEB level are missing. To better understand the physiological mechanism of NEB, milk of cows subjected to different dry period lengths, in different energy balance status and lactation stage, were analyzed by untargeted metabolomics and proteomics techniques. Milk of cows in severe NEB showed higher concentrations of acute phase response proteins, unsaturated fatty acids, and galactose-1-phosphate. Improved energy balance (EB) resulted in higher concentration of cholesterol, cholesterol synthesis related proteins, and stomatin. The presence of stomatin and galactose-1-phosphate in milk was strongly dependent on the EB of the cows. These novel and interesting findings warrant more in-depth research to assess their applicability as robust indicators of NEB in milk and to clarify the role of stomatin and galactose-1-phophate in milk of dairy cows in NEB.
LanguageEnglish
Pages3288-3296
JournalJournal of Proteome Research
Volume12
Issue number7
DOIs
Publication statusPublished - 2013

Fingerprint

Metabolome
Dairies
Proteome
Energy balance
Lactation
Milk
Parturition
Cholesterol
Ketosis
Acute-Phase Reaction
Metabolomics
Acute-Phase Proteins
Disease Susceptibility
Metabolic Diseases
Energy Intake
Unsaturated Fatty Acids
Galactose
Proteomics
Maintenance
Proteins

Keywords

  • mouse mammary-gland
  • epithelial-cells
  • fatty-acids
  • inflammatory response
  • beta-hydroxybutyrate
  • ketone-bodies
  • liver
  • apoptosis
  • stomatin
  • health

Cite this

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title = "Changes in milk proteome and metabolome associated with dry period length, energy balance and lactation stage in post parturient dairy cows",
abstract = "The early lactation period of dairy cows, which produce high quantities of milk, is normally characterized by an insufficient energy intake to cover milk production and maintenance requirements. Mobilization of body reserves occurs to compensate this negative energy balance (NEB), and probably as a consequence there is a higher susceptibility to diseases and metabolic disorders. There are several diagnostic methods to detect NEB, usually involving ketosis related parameters. Due to the easy availability of milk this is a preferred matrix, but simple and robust predictors of NEB level are missing. To better understand the physiological mechanism of NEB, milk of cows subjected to different dry period lengths, in different energy balance status and lactation stage, were analyzed by untargeted metabolomics and proteomics techniques. Milk of cows in severe NEB showed higher concentrations of acute phase response proteins, unsaturated fatty acids, and galactose-1-phosphate. Improved energy balance (EB) resulted in higher concentration of cholesterol, cholesterol synthesis related proteins, and stomatin. The presence of stomatin and galactose-1-phosphate in milk was strongly dependent on the EB of the cows. These novel and interesting findings warrant more in-depth research to assess their applicability as robust indicators of NEB in milk and to clarify the role of stomatin and galactose-1-phophate in milk of dairy cows in NEB.",
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author = "J. Lu and {Antunes Fernandes}, E.C. and {P{\'a}ez Cano}, A.E. and J. Vinitwatanakhun and S. Boeren and {van Hooijdonk}, A.C.M. and {van Knegsel}, A.T.M. and J. Vervoort and K.A. Hettinga",
year = "2013",
doi = "10.1021/pr4001306",
language = "English",
volume = "12",
pages = "3288--3296",
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publisher = "American Chemical Society",
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}

Changes in milk proteome and metabolome associated with dry period length, energy balance and lactation stage in post parturient dairy cows. / Lu, J.; Antunes Fernandes, E.C.; Páez Cano, A.E.; Vinitwatanakhun, J.; Boeren, S.; van Hooijdonk, A.C.M.; van Knegsel, A.T.M.; Vervoort, J.; Hettinga, K.A.

In: Journal of Proteome Research, Vol. 12, No. 7, 2013, p. 3288-3296.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Changes in milk proteome and metabolome associated with dry period length, energy balance and lactation stage in post parturient dairy cows

AU - Lu, J.

AU - Antunes Fernandes, E.C.

AU - Páez Cano, A.E.

AU - Vinitwatanakhun, J.

AU - Boeren, S.

AU - van Hooijdonk, A.C.M.

AU - van Knegsel, A.T.M.

AU - Vervoort, J.

AU - Hettinga, K.A.

PY - 2013

Y1 - 2013

N2 - The early lactation period of dairy cows, which produce high quantities of milk, is normally characterized by an insufficient energy intake to cover milk production and maintenance requirements. Mobilization of body reserves occurs to compensate this negative energy balance (NEB), and probably as a consequence there is a higher susceptibility to diseases and metabolic disorders. There are several diagnostic methods to detect NEB, usually involving ketosis related parameters. Due to the easy availability of milk this is a preferred matrix, but simple and robust predictors of NEB level are missing. To better understand the physiological mechanism of NEB, milk of cows subjected to different dry period lengths, in different energy balance status and lactation stage, were analyzed by untargeted metabolomics and proteomics techniques. Milk of cows in severe NEB showed higher concentrations of acute phase response proteins, unsaturated fatty acids, and galactose-1-phosphate. Improved energy balance (EB) resulted in higher concentration of cholesterol, cholesterol synthesis related proteins, and stomatin. The presence of stomatin and galactose-1-phosphate in milk was strongly dependent on the EB of the cows. These novel and interesting findings warrant more in-depth research to assess their applicability as robust indicators of NEB in milk and to clarify the role of stomatin and galactose-1-phophate in milk of dairy cows in NEB.

AB - The early lactation period of dairy cows, which produce high quantities of milk, is normally characterized by an insufficient energy intake to cover milk production and maintenance requirements. Mobilization of body reserves occurs to compensate this negative energy balance (NEB), and probably as a consequence there is a higher susceptibility to diseases and metabolic disorders. There are several diagnostic methods to detect NEB, usually involving ketosis related parameters. Due to the easy availability of milk this is a preferred matrix, but simple and robust predictors of NEB level are missing. To better understand the physiological mechanism of NEB, milk of cows subjected to different dry period lengths, in different energy balance status and lactation stage, were analyzed by untargeted metabolomics and proteomics techniques. Milk of cows in severe NEB showed higher concentrations of acute phase response proteins, unsaturated fatty acids, and galactose-1-phosphate. Improved energy balance (EB) resulted in higher concentration of cholesterol, cholesterol synthesis related proteins, and stomatin. The presence of stomatin and galactose-1-phosphate in milk was strongly dependent on the EB of the cows. These novel and interesting findings warrant more in-depth research to assess their applicability as robust indicators of NEB in milk and to clarify the role of stomatin and galactose-1-phophate in milk of dairy cows in NEB.

KW - mouse mammary-gland

KW - epithelial-cells

KW - fatty-acids

KW - inflammatory response

KW - beta-hydroxybutyrate

KW - ketone-bodies

KW - liver

KW - apoptosis

KW - stomatin

KW - health

U2 - 10.1021/pr4001306

DO - 10.1021/pr4001306

M3 - Article

VL - 12

SP - 3288

EP - 3296

JO - Journal of Proteome Research

T2 - Journal of Proteome Research

JF - Journal of Proteome Research

SN - 1535-3893

IS - 7

ER -