Changes in gene expresssion during male meiosis in Petunia hybrida

F. Cnudde, V. Hedatale, J.H.S.G.M. de Jong, E.S. Pierson, D.Y. Rainey, M. Zabeau, K. Weterings, T. Gerats, J.L. Peters

Research output: Contribution to journalArticleAcademicpeer-review

22 Citations (Scopus)

Abstract

We analyzed changes in gene expression during male meiosis in Petunia by combining the meiotic staging of pollen mother cells from a single anther with cDNA-AFLP transcript profiling of mRNA from the synchronously developing sister anthers. The transcript profiling experiments focused on the identification of genes with a modulated expression profile during meiosis, while premeiotic archesporial cells and postmeiotic microspores served as a reference. About 8000 transcript tags, estimated at 30% of the total transcriptome, were generated, of which around 6% exhibited a modulated gene expression pattern at meiosis. Cluster analysis revealed a transcriptional cascade that coincides with the initiation and progression through all stages of the two meiotic divisions. Fragments that exhibited high expression specifically during meiosis I were characterized further by sequencing; 90 out of the 293 sequenced fragments showed homology with known genes, belonging to a wide range of gene classes, including previously characterized meiotic genes. In-situ hybridization experiments were performed to determine the spatial expression pattern for five selected transcript tags. Its concurrence with cDNA-AFLP transcript profiles indicates that this is an excellent approach to study genes involved in specialized processes such as meiosis. Our data set provides the potential to unravel unique meiotic genes that are as yet elusive to reverse genetics approaches
Original languageEnglish
Pages (from-to)919-932
JournalChromosome Research
Volume14
Issue number8
DOIs
Publication statusPublished - 2006

Keywords

  • genome-wide expression
  • arabidopsis-thaliana
  • chromosome synapsis
  • meiotic prophase
  • transcriptional program
  • spermatogenic cells
  • spindle checkpoint
  • fission yeast
  • homolog
  • protein

Fingerprint Dive into the research topics of 'Changes in gene expresssion during male meiosis in Petunia hybrida'. Together they form a unique fingerprint.

  • Cite this

    Cnudde, F., Hedatale, V., de Jong, J. H. S. G. M., Pierson, E. S., Rainey, D. Y., Zabeau, M., Weterings, K., Gerats, T., & Peters, J. L. (2006). Changes in gene expresssion during male meiosis in Petunia hybrida. Chromosome Research, 14(8), 919-932. https://doi.org/10.1007/s10577-006-1099-5