TY - JOUR
T1 - Changes in faecal microbiota in UC patients after Faecal Microbiota Transplantation
AU - Rossen, N.
AU - Fuentes Enriquez de Salamanca, S.
AU - Spek, M.
AU - Hartman, J.H.A.
AU - de Vos, W.M.
AU - D'Haens, G.R.
AU - Zoetendal, E.G.
AU - Ponsioen, C.Y.
PY - 2015
Y1 - 2015
N2 - Background
A disturbed gut microbiota is assumed to play a crucial role in the chronic inflammation in ulcerative colitis (UC). A radical way to interfere with the intestinal microbiota is faecal microbiota transplantation (FMT) using faeces from a healthy donor. The aim of our study was to study signature changes upon FMT in UC patients who did and did not respond to FMT.
Methods
Faecal samples from 37 patients who participated in the TURN trial and samples from corresponding donors were used for microbiota profiling. In this trial, active UC patients were 1:1 randomised to duodenal infusion of FMT derived from donor faeces (FMT-D) or patients' own faeces (FMT-P) after bowel lavage. Twelve patients (FMT-D: 7/17 patients, FMT-P: 5/20) achieved remission ('responders') at week 12 and 25 did not ('non-responders'). Composition and diversity of the microbiota from donors and recipients were compared and contrasted phylogenetic microarray analysis of 16S rRNA amplions by means of the Human Intestinal Tract Chip.
Results
Twelve weeks after treatment fecal microbiota diversity increased significantly in all responders (n=12), (fig.1). In the FMT-D group the similarity index of recipients to their respective donors had significantly increased at week 12 in responders, whilst this was not seen in the non-responders (fig. 2). Moreover, at 12 weeks the similarity to corresponding donors was significantly higher in responders versus non-responders. The differences at week 12 between responders and non-responders were exclusively explained by a 2-4 fold increase in abundance of Clostridium cluster XIVa members in responders (p
AB - Background
A disturbed gut microbiota is assumed to play a crucial role in the chronic inflammation in ulcerative colitis (UC). A radical way to interfere with the intestinal microbiota is faecal microbiota transplantation (FMT) using faeces from a healthy donor. The aim of our study was to study signature changes upon FMT in UC patients who did and did not respond to FMT.
Methods
Faecal samples from 37 patients who participated in the TURN trial and samples from corresponding donors were used for microbiota profiling. In this trial, active UC patients were 1:1 randomised to duodenal infusion of FMT derived from donor faeces (FMT-D) or patients' own faeces (FMT-P) after bowel lavage. Twelve patients (FMT-D: 7/17 patients, FMT-P: 5/20) achieved remission ('responders') at week 12 and 25 did not ('non-responders'). Composition and diversity of the microbiota from donors and recipients were compared and contrasted phylogenetic microarray analysis of 16S rRNA amplions by means of the Human Intestinal Tract Chip.
Results
Twelve weeks after treatment fecal microbiota diversity increased significantly in all responders (n=12), (fig.1). In the FMT-D group the similarity index of recipients to their respective donors had significantly increased at week 12 in responders, whilst this was not seen in the non-responders (fig. 2). Moreover, at 12 weeks the similarity to corresponding donors was significantly higher in responders versus non-responders. The differences at week 12 between responders and non-responders were exclusively explained by a 2-4 fold increase in abundance of Clostridium cluster XIVa members in responders (p
U2 - 10.1093/ecco-jcc/jju027.116
DO - 10.1093/ecco-jcc/jju027.116
M3 - Abstract
SN - 1873-9946
VL - 9
SP - S73-S74
JO - Journal of Crohn's and Colitis
JF - Journal of Crohn's and Colitis
IS - S1
ER -