Changes in faecal microbiota in UC patients after Faecal Microbiota Transplantation

N. Rossen, S. Fuentes Enriquez de Salamanca, M. Spek, J.H.A. Hartman, W.M. de Vos, G.R. D'Haens, E.G. Zoetendal, C.Y. Ponsioen

Research output: Contribution to journalAbstractAcademic

Abstract

Background A disturbed gut microbiota is assumed to play a crucial role in the chronic inflammation in ulcerative colitis (UC). A radical way to interfere with the intestinal microbiota is faecal microbiota transplantation (FMT) using faeces from a healthy donor. The aim of our study was to study signature changes upon FMT in UC patients who did and did not respond to FMT. Methods Faecal samples from 37 patients who participated in the TURN trial and samples from corresponding donors were used for microbiota profiling. In this trial, active UC patients were 1:1 randomised to duodenal infusion of FMT derived from donor faeces (FMT-D) or patients' own faeces (FMT-P) after bowel lavage. Twelve patients (FMT-D: 7/17 patients, FMT-P: 5/20) achieved remission ('responders') at week 12 and 25 did not ('non-responders'). Composition and diversity of the microbiota from donors and recipients were compared and contrasted phylogenetic microarray analysis of 16S rRNA amplions by means of the Human Intestinal Tract Chip. Results Twelve weeks after treatment fecal microbiota diversity increased significantly in all responders (n=12), (fig.1). In the FMT-D group the similarity index of recipients to their respective donors had significantly increased at week 12 in responders, whilst this was not seen in the non-responders (fig. 2). Moreover, at 12 weeks the similarity to corresponding donors was significantly higher in responders versus non-responders. The differences at week 12 between responders and non-responders were exclusively explained by a 2-4 fold increase in abundance of Clostridium cluster XIVa members in responders (p
Original languageEnglish
Pages (from-to)S73-S74
Number of pages2
JournalJournal of Crohn's and Colitis
Volume9
Issue numberS1
DOIs
Publication statusPublished - 2015

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