Central Cell-Derived Peptides Regulate Early Embryo Patterning in Flowering Plants

L.M. Costa, E. Marshall, M. Tesfaye, K.A.T. Silverstein, M. Mori, Y. Umetsu, S.L. Otterbach, R. Papareddy, H.G. Dickinson, K.A. Boutilier, K.A. VandenBosch, S. Ohki, J.F. Gutierrez-Marcos

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Abstract

Plant embryogenesis initiates with the establishment of an apical-basal axis; however, the molecular mechanisms accompanying this early event remain unclear. Here, we show that a small cysteine-rich peptide family is required for formation of the zygotic basal cell lineage and proembryo patterning in Arabidopsis. EMBRYO SURROUNDING FACTOR 1 (ESF1) peptides accumulate before fertilization in central cell gametes and thereafter in embryo-surrounding endosperm cells. Biochemical and structural analyses revealed cleavage of ESF1 propeptides to form biologically active mature peptides. Further, these peptides act in a non–cell-autonomous manner and synergistically with the receptor-like kinase SHORT SUSPENSOR to promote suspensor elongation through the YODA mitogen-activated protein kinase pathway. Our findings demonstrate that the second female gamete and its sexually derived endosperm regulate early embryonic patterning in flowering plants.
Original languageEnglish
Pages (from-to)168-172
JournalScience
Volume344
Issue number6180
DOIs
Publication statusPublished - 2014

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Keywords

  • apical-basal axis
  • double fertilization
  • stomatal density
  • gene-expression
  • arabidopsis
  • embryogenesis
  • endosperm
  • communication
  • infection
  • origin

Cite this

Costa, L. M., Marshall, E., Tesfaye, M., Silverstein, K. A. T., Mori, M., Umetsu, Y., ... Gutierrez-Marcos, J. F. (2014). Central Cell-Derived Peptides Regulate Early Embryo Patterning in Flowering Plants. Science, 344(6180), 168-172. https://doi.org/10.1126/science.1243005