Cellular signaling and cytoskeleton dynamics in Phytophthora

F. Govers, D.J. van den Hoogen, K. Kots, T. Ketelaar

Research output: Contribution to conferenceAbstractAcademic

Abstract

Sensing external signals and transducing these into intracellular responses
requires a balanced cellular organization that is largely governed by signal
transduction pathways and cytoskeleton dynamics. We aim to gain more
insight in these processes in Phytophthora species, oomycete plant pathogens
that cause devastating diseases worldwide. Our studies have shown that
Phytophthora spp. and other oomycetes possess unique classes of
GPCR-bigrams, proteins in which a G-protein coupled receptor (GPCR)
domain is linked to an accessory domain. Examples are GPCR-PIPKs that
have a phosphatidylinositol kinase (PIPK) domain as accessory domain. This
points to a direct connection between the two most important eukaryotic
signaling pathways, G-protein signaling and phospholipid signaling. In yeast
PIP2 generated by PIPK influences actin dynamics but in Phytophthora the
relation between PIP2, PIPK and actin is unknown. Actin is a major
component of the cytoskeleton, a well-organized, dynamic system of
intracellular filaments primary composed of actin and tubulin. Previously we
identified actin plaques as highly immobile, long-lived structures that are
unique for oomycetes. In addition, we found two other unique actin
configurations; one associated with plug deposition in germ tubes and the
other with appressoria, infection structures formed prior to host cell
penetration. Here we will present an update on GPCR-bigrams, the functional analyses of the Gγ subunit of the heterotrimeric G-protein, and new insights in
nuclear division based on life cell imaging of the microtubule cytoskeleton.
Our long-term goal is to uncover oomycete or Phytophthora specific features
that might be instrumental for drug design.
Original languageEnglish
Publication statusPublished - 2018
EventOomycete Molecular Genetics Network Meeting 2018 - Taian, Shandong, China
Duration: 8 Apr 201812 Apr 2018

Conference

ConferenceOomycete Molecular Genetics Network Meeting 2018
CountryChina
CityTaian, Shandong
Period8/04/1812/04/18

Fingerprint

Oomycetes
Phytophthora
Cytoskeleton
G-Protein-Coupled Receptors
Phosphatidylinositols
Actins
Heterotrimeric GTP-Binding Proteins
Drug Design
Tubulin
GTP-Binding Proteins
Microtubules
Phospholipids
Phosphotransferases
Infection
Proteins
actin kinase

Cite this

Govers, F., van den Hoogen, D. J., Kots, K., & Ketelaar, T. (2018). Cellular signaling and cytoskeleton dynamics in Phytophthora. Abstract from Oomycete Molecular Genetics Network Meeting 2018, Taian, Shandong, China.
Govers, F. ; van den Hoogen, D.J. ; Kots, K. ; Ketelaar, T. / Cellular signaling and cytoskeleton dynamics in Phytophthora. Abstract from Oomycete Molecular Genetics Network Meeting 2018, Taian, Shandong, China.
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title = "Cellular signaling and cytoskeleton dynamics in Phytophthora",
abstract = "Sensing external signals and transducing these into intracellular responsesrequires a balanced cellular organization that is largely governed by signaltransduction pathways and cytoskeleton dynamics. We aim to gain moreinsight in these processes in Phytophthora species, oomycete plant pathogensthat cause devastating diseases worldwide. Our studies have shown thatPhytophthora spp. and other oomycetes possess unique classes ofGPCR-bigrams, proteins in which a G-protein coupled receptor (GPCR)domain is linked to an accessory domain. Examples are GPCR-PIPKs thathave a phosphatidylinositol kinase (PIPK) domain as accessory domain. Thispoints to a direct connection between the two most important eukaryoticsignaling pathways, G-protein signaling and phospholipid signaling. In yeastPIP2 generated by PIPK influences actin dynamics but in Phytophthora therelation between PIP2, PIPK and actin is unknown. Actin is a majorcomponent of the cytoskeleton, a well-organized, dynamic system ofintracellular filaments primary composed of actin and tubulin. Previously weidentified actin plaques as highly immobile, long-lived structures that areunique for oomycetes. In addition, we found two other unique actinconfigurations; one associated with plug deposition in germ tubes and theother with appressoria, infection structures formed prior to host cellpenetration. Here we will present an update on GPCR-bigrams, the functional analyses of the Gγ subunit of the heterotrimeric G-protein, and new insights innuclear division based on life cell imaging of the microtubule cytoskeleton.Our long-term goal is to uncover oomycete or Phytophthora specific featuresthat might be instrumental for drug design.",
author = "F. Govers and {van den Hoogen}, D.J. and K. Kots and T. Ketelaar",
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language = "English",
note = "Oomycete Molecular Genetics Network Meeting 2018 ; Conference date: 08-04-2018 Through 12-04-2018",

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Govers, F, van den Hoogen, DJ, Kots, K & Ketelaar, T 2018, 'Cellular signaling and cytoskeleton dynamics in Phytophthora' Oomycete Molecular Genetics Network Meeting 2018, Taian, Shandong, China, 8/04/18 - 12/04/18, .

Cellular signaling and cytoskeleton dynamics in Phytophthora. / Govers, F.; van den Hoogen, D.J.; Kots, K.; Ketelaar, T.

2018. Abstract from Oomycete Molecular Genetics Network Meeting 2018, Taian, Shandong, China.

Research output: Contribution to conferenceAbstractAcademic

TY - CONF

T1 - Cellular signaling and cytoskeleton dynamics in Phytophthora

AU - Govers, F.

AU - van den Hoogen, D.J.

AU - Kots, K.

AU - Ketelaar, T.

PY - 2018

Y1 - 2018

N2 - Sensing external signals and transducing these into intracellular responsesrequires a balanced cellular organization that is largely governed by signaltransduction pathways and cytoskeleton dynamics. We aim to gain moreinsight in these processes in Phytophthora species, oomycete plant pathogensthat cause devastating diseases worldwide. Our studies have shown thatPhytophthora spp. and other oomycetes possess unique classes ofGPCR-bigrams, proteins in which a G-protein coupled receptor (GPCR)domain is linked to an accessory domain. Examples are GPCR-PIPKs thathave a phosphatidylinositol kinase (PIPK) domain as accessory domain. Thispoints to a direct connection between the two most important eukaryoticsignaling pathways, G-protein signaling and phospholipid signaling. In yeastPIP2 generated by PIPK influences actin dynamics but in Phytophthora therelation between PIP2, PIPK and actin is unknown. Actin is a majorcomponent of the cytoskeleton, a well-organized, dynamic system ofintracellular filaments primary composed of actin and tubulin. Previously weidentified actin plaques as highly immobile, long-lived structures that areunique for oomycetes. In addition, we found two other unique actinconfigurations; one associated with plug deposition in germ tubes and theother with appressoria, infection structures formed prior to host cellpenetration. Here we will present an update on GPCR-bigrams, the functional analyses of the Gγ subunit of the heterotrimeric G-protein, and new insights innuclear division based on life cell imaging of the microtubule cytoskeleton.Our long-term goal is to uncover oomycete or Phytophthora specific featuresthat might be instrumental for drug design.

AB - Sensing external signals and transducing these into intracellular responsesrequires a balanced cellular organization that is largely governed by signaltransduction pathways and cytoskeleton dynamics. We aim to gain moreinsight in these processes in Phytophthora species, oomycete plant pathogensthat cause devastating diseases worldwide. Our studies have shown thatPhytophthora spp. and other oomycetes possess unique classes ofGPCR-bigrams, proteins in which a G-protein coupled receptor (GPCR)domain is linked to an accessory domain. Examples are GPCR-PIPKs thathave a phosphatidylinositol kinase (PIPK) domain as accessory domain. Thispoints to a direct connection between the two most important eukaryoticsignaling pathways, G-protein signaling and phospholipid signaling. In yeastPIP2 generated by PIPK influences actin dynamics but in Phytophthora therelation between PIP2, PIPK and actin is unknown. Actin is a majorcomponent of the cytoskeleton, a well-organized, dynamic system ofintracellular filaments primary composed of actin and tubulin. Previously weidentified actin plaques as highly immobile, long-lived structures that areunique for oomycetes. In addition, we found two other unique actinconfigurations; one associated with plug deposition in germ tubes and theother with appressoria, infection structures formed prior to host cellpenetration. Here we will present an update on GPCR-bigrams, the functional analyses of the Gγ subunit of the heterotrimeric G-protein, and new insights innuclear division based on life cell imaging of the microtubule cytoskeleton.Our long-term goal is to uncover oomycete or Phytophthora specific featuresthat might be instrumental for drug design.

M3 - Abstract

ER -

Govers F, van den Hoogen DJ, Kots K, Ketelaar T. Cellular signaling and cytoskeleton dynamics in Phytophthora. 2018. Abstract from Oomycete Molecular Genetics Network Meeting 2018, Taian, Shandong, China.