Cellphone-based detection platform for rbST biomerker analysis in milk extracts using a microsphere fluorescence immunoassay

S.K.J. Ludwig, H. Zhu, S. Phillips, A. Shiledar, S. Feng, D. Tseng, L.A. van Ginkel, M.W.F. Nielen, A. Ozcan

Research output: Contribution to journalArticleAcademicpeer-review

47 Citations (Scopus)

Abstract

Current contaminant and residue monitoring throughout the food chain is based on sampling, transport, administration, and analysis in specialized control laboratories. This is a highly inefficient and costly process since typically more than 99 % of the samples are found to be compliant. On-site simplified prescreening may provide a scenario in which only samples that are suspect are transported and further processed. Such a prescreening can be performed using a small attachment on a cellphone. To this end, a cellphone-based imaging platform for a microsphere fluorescence immunoassay that detects the presence of anti-recombinant bovine somatotropin (rbST) antibodies in milk extracts was developed. RbST administration to cows increases their milk production, but is illegal in the EU and a public health concern in the USA. The cellphone monitors the presence of anti-rbST antibodies (rbST biomarker), which are endogenously produced upon administration of rbST and excreted in milk. The rbST biomarker present in milk extracts was captured by rbST covalently coupled to paramagnetic microspheres and labeled by quantum dot (QD)-coupled detection antibodies. The emitted fluorescence light from these captured QDs was then imaged using the cellphone camera. Additionally, a dark-field image was taken in which all microspheres present were visible. The fluorescence and dark-field microimages were analyzed using a custom-developed Android application running on the same cellphone. With this setup, the microsphere fluorescence immunoassay and cellphone-based detection were successfully applied to milk sample extracts from rbST-treated and untreated cows. An 80 % true-positive rate and 95 % true-negative rate were achieved using this setup. Next, the cellphone-based detection platform was benchmarked against a newly developed planar imaging array alternative and found to be equally performing versus the much more sophisticated alternative. Using cellphone-based on-site analysis in future residue monitoring can limit the number of samples for laboratory analysis already at an early stage. Therewith, the entire monitoring process can become much more efficient and economical.
Original languageEnglish
Pages (from-to)6857-6866
Number of pages10
JournalAnalytical and Bioanalytical Chemistry
Volume406
Issue number27
DOIs
Publication statusPublished - 2014

Fingerprint

Microspheres
Immunoassay
Growth Hormone
Milk
Fluorescence
Biomarkers
Antibodies
Imaging techniques
Quantum Dots
Food Chain
Monitoring
Process monitoring
Public health
bovine growth hormone
Semiconductor quantum dots
Public Health
Cameras
Impurities
Sampling
Light

Keywords

  • recombinant bovine somatotropin
  • of-care diagnostics
  • cell-phone
  • smart-phone
  • microscopy
  • cost

Cite this

Ludwig, S.K.J. ; Zhu, H. ; Phillips, S. ; Shiledar, A. ; Feng, S. ; Tseng, D. ; van Ginkel, L.A. ; Nielen, M.W.F. ; Ozcan, A. / Cellphone-based detection platform for rbST biomerker analysis in milk extracts using a microsphere fluorescence immunoassay. In: Analytical and Bioanalytical Chemistry. 2014 ; Vol. 406, No. 27. pp. 6857-6866.
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abstract = "Current contaminant and residue monitoring throughout the food chain is based on sampling, transport, administration, and analysis in specialized control laboratories. This is a highly inefficient and costly process since typically more than 99 {\%} of the samples are found to be compliant. On-site simplified prescreening may provide a scenario in which only samples that are suspect are transported and further processed. Such a prescreening can be performed using a small attachment on a cellphone. To this end, a cellphone-based imaging platform for a microsphere fluorescence immunoassay that detects the presence of anti-recombinant bovine somatotropin (rbST) antibodies in milk extracts was developed. RbST administration to cows increases their milk production, but is illegal in the EU and a public health concern in the USA. The cellphone monitors the presence of anti-rbST antibodies (rbST biomarker), which are endogenously produced upon administration of rbST and excreted in milk. The rbST biomarker present in milk extracts was captured by rbST covalently coupled to paramagnetic microspheres and labeled by quantum dot (QD)-coupled detection antibodies. The emitted fluorescence light from these captured QDs was then imaged using the cellphone camera. Additionally, a dark-field image was taken in which all microspheres present were visible. The fluorescence and dark-field microimages were analyzed using a custom-developed Android application running on the same cellphone. With this setup, the microsphere fluorescence immunoassay and cellphone-based detection were successfully applied to milk sample extracts from rbST-treated and untreated cows. An 80 {\%} true-positive rate and 95 {\%} true-negative rate were achieved using this setup. Next, the cellphone-based detection platform was benchmarked against a newly developed planar imaging array alternative and found to be equally performing versus the much more sophisticated alternative. Using cellphone-based on-site analysis in future residue monitoring can limit the number of samples for laboratory analysis already at an early stage. Therewith, the entire monitoring process can become much more efficient and economical.",
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Cellphone-based detection platform for rbST biomerker analysis in milk extracts using a microsphere fluorescence immunoassay. / Ludwig, S.K.J.; Zhu, H.; Phillips, S.; Shiledar, A.; Feng, S.; Tseng, D.; van Ginkel, L.A.; Nielen, M.W.F.; Ozcan, A.

In: Analytical and Bioanalytical Chemistry, Vol. 406, No. 27, 2014, p. 6857-6866.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Cellphone-based detection platform for rbST biomerker analysis in milk extracts using a microsphere fluorescence immunoassay

AU - Ludwig, S.K.J.

AU - Zhu, H.

AU - Phillips, S.

AU - Shiledar, A.

AU - Feng, S.

AU - Tseng, D.

AU - van Ginkel, L.A.

AU - Nielen, M.W.F.

AU - Ozcan, A.

PY - 2014

Y1 - 2014

N2 - Current contaminant and residue monitoring throughout the food chain is based on sampling, transport, administration, and analysis in specialized control laboratories. This is a highly inefficient and costly process since typically more than 99 % of the samples are found to be compliant. On-site simplified prescreening may provide a scenario in which only samples that are suspect are transported and further processed. Such a prescreening can be performed using a small attachment on a cellphone. To this end, a cellphone-based imaging platform for a microsphere fluorescence immunoassay that detects the presence of anti-recombinant bovine somatotropin (rbST) antibodies in milk extracts was developed. RbST administration to cows increases their milk production, but is illegal in the EU and a public health concern in the USA. The cellphone monitors the presence of anti-rbST antibodies (rbST biomarker), which are endogenously produced upon administration of rbST and excreted in milk. The rbST biomarker present in milk extracts was captured by rbST covalently coupled to paramagnetic microspheres and labeled by quantum dot (QD)-coupled detection antibodies. The emitted fluorescence light from these captured QDs was then imaged using the cellphone camera. Additionally, a dark-field image was taken in which all microspheres present were visible. The fluorescence and dark-field microimages were analyzed using a custom-developed Android application running on the same cellphone. With this setup, the microsphere fluorescence immunoassay and cellphone-based detection were successfully applied to milk sample extracts from rbST-treated and untreated cows. An 80 % true-positive rate and 95 % true-negative rate were achieved using this setup. Next, the cellphone-based detection platform was benchmarked against a newly developed planar imaging array alternative and found to be equally performing versus the much more sophisticated alternative. Using cellphone-based on-site analysis in future residue monitoring can limit the number of samples for laboratory analysis already at an early stage. Therewith, the entire monitoring process can become much more efficient and economical.

AB - Current contaminant and residue monitoring throughout the food chain is based on sampling, transport, administration, and analysis in specialized control laboratories. This is a highly inefficient and costly process since typically more than 99 % of the samples are found to be compliant. On-site simplified prescreening may provide a scenario in which only samples that are suspect are transported and further processed. Such a prescreening can be performed using a small attachment on a cellphone. To this end, a cellphone-based imaging platform for a microsphere fluorescence immunoassay that detects the presence of anti-recombinant bovine somatotropin (rbST) antibodies in milk extracts was developed. RbST administration to cows increases their milk production, but is illegal in the EU and a public health concern in the USA. The cellphone monitors the presence of anti-rbST antibodies (rbST biomarker), which are endogenously produced upon administration of rbST and excreted in milk. The rbST biomarker present in milk extracts was captured by rbST covalently coupled to paramagnetic microspheres and labeled by quantum dot (QD)-coupled detection antibodies. The emitted fluorescence light from these captured QDs was then imaged using the cellphone camera. Additionally, a dark-field image was taken in which all microspheres present were visible. The fluorescence and dark-field microimages were analyzed using a custom-developed Android application running on the same cellphone. With this setup, the microsphere fluorescence immunoassay and cellphone-based detection were successfully applied to milk sample extracts from rbST-treated and untreated cows. An 80 % true-positive rate and 95 % true-negative rate were achieved using this setup. Next, the cellphone-based detection platform was benchmarked against a newly developed planar imaging array alternative and found to be equally performing versus the much more sophisticated alternative. Using cellphone-based on-site analysis in future residue monitoring can limit the number of samples for laboratory analysis already at an early stage. Therewith, the entire monitoring process can become much more efficient and economical.

KW - recombinant bovine somatotropin

KW - of-care diagnostics

KW - cell-phone

KW - smart-phone

KW - microscopy

KW - cost

U2 - 10.1007/s00216-014-7984-4

DO - 10.1007/s00216-014-7984-4

M3 - Article

VL - 406

SP - 6857

EP - 6866

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 27

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