Cell wall disruption increases bioavailability of Nannochloropsis gaditana nutrients for juvenile Nile tilapia (Oreochromis niloticus)

Emma Teuling, Peter A. Wierenga, Jeleel O. Agboola, Harry Gruppen, Johan W. Schrama

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

In this study the correlation between the accessibility of nutrients and in vivo nutrient digestibility was tested on the marine microalga Nannochloropsis gaditana in juvenile Nile tilapia (Oreochromis niloticus). It was hypothesized that disrupting the cell walls of microalgae increases the nutrient accessibility and digestibility. N. gaditana biomass was subjected to physical treatments (pasteurization, freezing, freeze drying) or mechanical treatments (bead milling) to influence its cell wall integrity. These treatments resulted in an up to 4 x increase in in vitro accessibility of N. gaditana nutrients, assessed from measurements of leaching and susceptibility to protein hydrolysis. Apparent digestibility coefficients of macronutrients, dry matter, energy, phosphorus and calcium of untreated and treated microalgae biomass were determined in triplicate, at a 30% diet inclusion level. Bead milling the algae led to the highest increase in in vivo digestibility of dry matter, energy, protein, fat, ash and calcium on ingredient level, compared to untreated algae biomass. This includes an increase in apparent digestibility coefficient (ADC) of protein and fat from 62 to 78% and from 50 to 82%, respectively. ADCs of total carbohydrates and of phosphorus were not affected by algal cell disruption. In vivo digestibilities of N. gaditana dry matter, energy, protein, and fat were positively correlated (p <.001; r ≥ 0.91) with the nutrient accessibility of N. gaditana, as estimated with in vitro nutrient leaching analyses. This shows that the in vitro methods used are effective ways to assess the effect of mechanical and physical treatments on in vivo nutrient quality of a single ingredient. The results of this study confirm that nutrient accessibility plays a significant role in the nutrient digestibility of the microalga Nannochloropsis gaditana in Nile tilapia.

LanguageEnglish
Pages269-282
JournalAquaculture
Volume499
DOIs
Publication statusPublished - 15 Jan 2019

Fingerprint

Nannochloropsis
Oreochromis niloticus
bioavailability
digestibility
cell walls
nutrient
nutrients
accessibility
algae
fat
dry matter
protein
microalga
microalgae
biomass
leaching
energy
ingredients
lipids
proteins

Keywords

  • Digestion
  • Feed ingredient
  • Maillard reaction products
  • Microalgae
  • Nutritive value

Cite this

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title = "Cell wall disruption increases bioavailability of Nannochloropsis gaditana nutrients for juvenile Nile tilapia (Oreochromis niloticus)",
abstract = "In this study the correlation between the accessibility of nutrients and in vivo nutrient digestibility was tested on the marine microalga Nannochloropsis gaditana in juvenile Nile tilapia (Oreochromis niloticus). It was hypothesized that disrupting the cell walls of microalgae increases the nutrient accessibility and digestibility. N. gaditana biomass was subjected to physical treatments (pasteurization, freezing, freeze drying) or mechanical treatments (bead milling) to influence its cell wall integrity. These treatments resulted in an up to 4 x increase in in vitro accessibility of N. gaditana nutrients, assessed from measurements of leaching and susceptibility to protein hydrolysis. Apparent digestibility coefficients of macronutrients, dry matter, energy, phosphorus and calcium of untreated and treated microalgae biomass were determined in triplicate, at a 30{\%} diet inclusion level. Bead milling the algae led to the highest increase in in vivo digestibility of dry matter, energy, protein, fat, ash and calcium on ingredient level, compared to untreated algae biomass. This includes an increase in apparent digestibility coefficient (ADC) of protein and fat from 62 to 78{\%} and from 50 to 82{\%}, respectively. ADCs of total carbohydrates and of phosphorus were not affected by algal cell disruption. In vivo digestibilities of N. gaditana dry matter, energy, protein, and fat were positively correlated (p <.001; r ≥ 0.91) with the nutrient accessibility of N. gaditana, as estimated with in vitro nutrient leaching analyses. This shows that the in vitro methods used are effective ways to assess the effect of mechanical and physical treatments on in vivo nutrient quality of a single ingredient. The results of this study confirm that nutrient accessibility plays a significant role in the nutrient digestibility of the microalga Nannochloropsis gaditana in Nile tilapia.",
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Cell wall disruption increases bioavailability of Nannochloropsis gaditana nutrients for juvenile Nile tilapia (Oreochromis niloticus). / Teuling, Emma; Wierenga, Peter A.; Agboola, Jeleel O.; Gruppen, Harry; Schrama, Johan W.

In: Aquaculture, Vol. 499, 15.01.2019, p. 269-282.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Cell wall disruption increases bioavailability of Nannochloropsis gaditana nutrients for juvenile Nile tilapia (Oreochromis niloticus)

AU - Teuling, Emma

AU - Wierenga, Peter A.

AU - Agboola, Jeleel O.

AU - Gruppen, Harry

AU - Schrama, Johan W.

PY - 2019/1/15

Y1 - 2019/1/15

N2 - In this study the correlation between the accessibility of nutrients and in vivo nutrient digestibility was tested on the marine microalga Nannochloropsis gaditana in juvenile Nile tilapia (Oreochromis niloticus). It was hypothesized that disrupting the cell walls of microalgae increases the nutrient accessibility and digestibility. N. gaditana biomass was subjected to physical treatments (pasteurization, freezing, freeze drying) or mechanical treatments (bead milling) to influence its cell wall integrity. These treatments resulted in an up to 4 x increase in in vitro accessibility of N. gaditana nutrients, assessed from measurements of leaching and susceptibility to protein hydrolysis. Apparent digestibility coefficients of macronutrients, dry matter, energy, phosphorus and calcium of untreated and treated microalgae biomass were determined in triplicate, at a 30% diet inclusion level. Bead milling the algae led to the highest increase in in vivo digestibility of dry matter, energy, protein, fat, ash and calcium on ingredient level, compared to untreated algae biomass. This includes an increase in apparent digestibility coefficient (ADC) of protein and fat from 62 to 78% and from 50 to 82%, respectively. ADCs of total carbohydrates and of phosphorus were not affected by algal cell disruption. In vivo digestibilities of N. gaditana dry matter, energy, protein, and fat were positively correlated (p <.001; r ≥ 0.91) with the nutrient accessibility of N. gaditana, as estimated with in vitro nutrient leaching analyses. This shows that the in vitro methods used are effective ways to assess the effect of mechanical and physical treatments on in vivo nutrient quality of a single ingredient. The results of this study confirm that nutrient accessibility plays a significant role in the nutrient digestibility of the microalga Nannochloropsis gaditana in Nile tilapia.

AB - In this study the correlation between the accessibility of nutrients and in vivo nutrient digestibility was tested on the marine microalga Nannochloropsis gaditana in juvenile Nile tilapia (Oreochromis niloticus). It was hypothesized that disrupting the cell walls of microalgae increases the nutrient accessibility and digestibility. N. gaditana biomass was subjected to physical treatments (pasteurization, freezing, freeze drying) or mechanical treatments (bead milling) to influence its cell wall integrity. These treatments resulted in an up to 4 x increase in in vitro accessibility of N. gaditana nutrients, assessed from measurements of leaching and susceptibility to protein hydrolysis. Apparent digestibility coefficients of macronutrients, dry matter, energy, phosphorus and calcium of untreated and treated microalgae biomass were determined in triplicate, at a 30% diet inclusion level. Bead milling the algae led to the highest increase in in vivo digestibility of dry matter, energy, protein, fat, ash and calcium on ingredient level, compared to untreated algae biomass. This includes an increase in apparent digestibility coefficient (ADC) of protein and fat from 62 to 78% and from 50 to 82%, respectively. ADCs of total carbohydrates and of phosphorus were not affected by algal cell disruption. In vivo digestibilities of N. gaditana dry matter, energy, protein, and fat were positively correlated (p <.001; r ≥ 0.91) with the nutrient accessibility of N. gaditana, as estimated with in vitro nutrient leaching analyses. This shows that the in vitro methods used are effective ways to assess the effect of mechanical and physical treatments on in vivo nutrient quality of a single ingredient. The results of this study confirm that nutrient accessibility plays a significant role in the nutrient digestibility of the microalga Nannochloropsis gaditana in Nile tilapia.

KW - Digestion

KW - Feed ingredient

KW - Maillard reaction products

KW - Microalgae

KW - Nutritive value

U2 - 10.1016/j.aquaculture.2018.09.047

DO - 10.1016/j.aquaculture.2018.09.047

M3 - Article

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SP - 269

EP - 282

JO - Aquaculture

T2 - Aquaculture

JF - Aquaculture

SN - 0044-8486

ER -