Projects per year
Abstract
Many developmental processes involve transitions between different cell identities as cells differentiate or undergo reprogramming. Cell identity specifications are generally associated with the activation and suppression of specific sets of genes mediated by transcription factors. Therefore, transcriptional reporters, such as promoters of cell-type-specific genes, are broadly used as cell identity markers in developmental biology. In Arabidopsis (Arabidopsis thaliana), a collection of GAL4/UAS enhancer trap lines is an established standard for inferring cell identity. However, only a few of these enhancer trap lines have been molecularly characterized, which limits their potential. Here, we describe an approach for a detailed characterization of expression and mapping of T-DNA insert location of GAL4/UAS enhancer trap lines. Additionally, we demonstrate how the acquired information can be further used for the generation of novel cell-type-specific promoters as well as for genotyping of enhancer trap lines.
Original language | English |
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Title of host publication | Plant Transcription Factors |
Subtitle of host publication | Methods and Protocols |
Publisher | Humana Press |
Pages | 127-139 |
ISBN (Electronic) | 9781493986576 |
ISBN (Print) | 9781493986569 |
DOIs | |
Publication status | Published - 25 Jul 2018 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 1830 |
ISSN (Print) | 1064-3745 |
Keywords
- Arabidopsis
- Cell-type-specific promoters
- Enhancer trap lines
- Expression analysis
- Insert location
- TAIL-PCR
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Dive into the research topics of 'Cell-type-specific promoter identification using enhancer trap lines'. Together they form a unique fingerprint.Projects
- 1 Finished
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CELLPATTERN: The Cellular Basis of Multicellular Pattern Formation
1/10/11 → 30/09/16
Project: EU research project