Abstract
Many developmental processes involve transitions between different cell identities as cells differentiate or undergo reprogramming. Cell identity specifications are generally associated with the activation and suppression of specific sets of genes mediated by transcription factors. Therefore, transcriptional reporters, such as promoters of cell-type-specific genes, are broadly used as cell identity markers in developmental biology. In Arabidopsis (Arabidopsis thaliana), a collection of GAL4/UAS enhancer trap lines is an established standard for inferring cell identity. However, only a few of these enhancer trap lines have been molecularly characterized, which limits their potential. Here, we describe an approach for a detailed characterization of expression and mapping of T-DNA insert location of GAL4/UAS enhancer trap lines. Additionally, we demonstrate how the acquired information can be further used for the generation of novel cell-type-specific promoters as well as for genotyping of enhancer trap lines.
| Language | English |
|---|---|
| Title of host publication | Plant Transcription Factors |
| Subtitle of host publication | Methods and Protocols |
| Publisher | Humana Press Inc. |
| Pages | 127-139 |
| ISBN (Electronic) | 9781493986576 |
| ISBN (Print) | 9781493986569 |
| DOIs | |
| Publication status | Published - 25 Jul 2018 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1830 |
| ISSN (Print) | 1064-3745 |
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Keywords
- Arabidopsis
- Cell-type-specific promoters
- Enhancer trap lines
- Expression analysis
- Insert location
- TAIL-PCR
Cite this
}
Cell-type-specific promoter identification using enhancer trap lines. / Radoeva, Tatyana; Saiga, Shunsuke; Weijers, Dolf.
Plant Transcription Factors: Methods and Protocols. Humana Press Inc., 2018. p. 127-139 (Methods in Molecular Biology; Vol. 1830).Research output: Chapter in Book/Report/Conference proceeding › Chapter › Academic › peer-review
TY - CHAP
T1 - Cell-type-specific promoter identification using enhancer trap lines
AU - Radoeva, Tatyana
AU - Saiga, Shunsuke
AU - Weijers, Dolf
PY - 2018/7/25
Y1 - 2018/7/25
N2 - Many developmental processes involve transitions between different cell identities as cells differentiate or undergo reprogramming. Cell identity specifications are generally associated with the activation and suppression of specific sets of genes mediated by transcription factors. Therefore, transcriptional reporters, such as promoters of cell-type-specific genes, are broadly used as cell identity markers in developmental biology. In Arabidopsis (Arabidopsis thaliana), a collection of GAL4/UAS enhancer trap lines is an established standard for inferring cell identity. However, only a few of these enhancer trap lines have been molecularly characterized, which limits their potential. Here, we describe an approach for a detailed characterization of expression and mapping of T-DNA insert location of GAL4/UAS enhancer trap lines. Additionally, we demonstrate how the acquired information can be further used for the generation of novel cell-type-specific promoters as well as for genotyping of enhancer trap lines.
AB - Many developmental processes involve transitions between different cell identities as cells differentiate or undergo reprogramming. Cell identity specifications are generally associated with the activation and suppression of specific sets of genes mediated by transcription factors. Therefore, transcriptional reporters, such as promoters of cell-type-specific genes, are broadly used as cell identity markers in developmental biology. In Arabidopsis (Arabidopsis thaliana), a collection of GAL4/UAS enhancer trap lines is an established standard for inferring cell identity. However, only a few of these enhancer trap lines have been molecularly characterized, which limits their potential. Here, we describe an approach for a detailed characterization of expression and mapping of T-DNA insert location of GAL4/UAS enhancer trap lines. Additionally, we demonstrate how the acquired information can be further used for the generation of novel cell-type-specific promoters as well as for genotyping of enhancer trap lines.
KW - Arabidopsis
KW - Cell-type-specific promoters
KW - Enhancer trap lines
KW - Expression analysis
KW - Insert location
KW - TAIL-PCR
U2 - 10.1007/978-1-4939-8657-6_8
DO - 10.1007/978-1-4939-8657-6_8
M3 - Chapter
SN - 9781493986569
T3 - Methods in Molecular Biology
SP - 127
EP - 139
BT - Plant Transcription Factors
PB - Humana Press Inc.
ER -