Cell-type-specific promoter identification using enhancer trap lines

Tatyana Radoeva, Shunsuke Saiga, Dolf Weijers*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

Abstract

Many developmental processes involve transitions between different cell identities as cells differentiate or undergo reprogramming. Cell identity specifications are generally associated with the activation and suppression of specific sets of genes mediated by transcription factors. Therefore, transcriptional reporters, such as promoters of cell-type-specific genes, are broadly used as cell identity markers in developmental biology. In Arabidopsis (Arabidopsis thaliana), a collection of GAL4/UAS enhancer trap lines is an established standard for inferring cell identity. However, only a few of these enhancer trap lines have been molecularly characterized, which limits their potential. Here, we describe an approach for a detailed characterization of expression and mapping of T-DNA insert location of GAL4/UAS enhancer trap lines. Additionally, we demonstrate how the acquired information can be further used for the generation of novel cell-type-specific promoters as well as for genotyping of enhancer trap lines.

Original languageEnglish
Title of host publicationPlant Transcription Factors
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages127-139
ISBN (Electronic)9781493986576
ISBN (Print)9781493986569
DOIs
Publication statusPublished - 25 Jul 2018

Publication series

NameMethods in Molecular Biology
Volume1830
ISSN (Print)1064-3745

Keywords

  • Arabidopsis
  • Cell-type-specific promoters
  • Enhancer trap lines
  • Expression analysis
  • Insert location
  • TAIL-PCR

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