Cell culture based production of avian influenza vaccines

R. van Wielink

Research output: Thesisinternal PhD, WU

Abstract

Vaccination of poultry can be used as a tool to control outbreaks of avian influenza, including that of highly pathogenic H5 and H7 strains. Influenza vaccines are traditionally produced in embryonated chicken eggs. Continuous cell lines have been suggested as an alternative substrate to produce influenza vaccines, as they are more robust and lack the long lead times associated with the production of large quantities of embryonated eggs. In the study that is described in this thesis, the production of influenza virus in cell culture was explored. Therefore, several cell lines were assessed for their ability to propagate influenza virus. Furthermore, adaptations to both cell line and seed virus were suggested that increased virus yield, thereby allowing the production of attenuated influenza virus strains.

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Wijffels, Rene, Promotor
  • Moormann, Rob, Promotor
  • Harmsen, Michael, Co-promotor
  • Martens, Dirk, Co-promotor
Award date19 Oct 2012
Place of PublicationS.l.
Publisher
Print ISBNs9789461733535
Publication statusPublished - 2012

Fingerprint

avian influenza
Orthomyxoviridae
cell culture
cell lines
vaccines
influenza
viruses
chicken eggs
poultry
vaccination
seeds

Keywords

  • avian influenza
  • avian influenza a viruses
  • cell culture vaccines
  • vaccines
  • cell culture
  • virology
  • bioprocess engineering
  • veterinary medicine

Cite this

van Wielink, R.. / Cell culture based production of avian influenza vaccines. S.l. : s.n., 2012. 141 p.
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title = "Cell culture based production of avian influenza vaccines",
abstract = "Vaccination of poultry can be used as a tool to control outbreaks of avian influenza, including that of highly pathogenic H5 and H7 strains. Influenza vaccines are traditionally produced in embryonated chicken eggs. Continuous cell lines have been suggested as an alternative substrate to produce influenza vaccines, as they are more robust and lack the long lead times associated with the production of large quantities of embryonated eggs. In the study that is described in this thesis, the production of influenza virus in cell culture was explored. Therefore, several cell lines were assessed for their ability to propagate influenza virus. Furthermore, adaptations to both cell line and seed virus were suggested that increased virus yield, thereby allowing the production of attenuated influenza virus strains.",
keywords = "aviaire influenza, aviaire influenza a-virussen, celcultuur vaccins, vaccins, celkweek, virologie, bioproceskunde, veeartsenijkunde, avian influenza, avian influenza a viruses, cell culture vaccines, vaccines, cell culture, virology, bioprocess engineering, veterinary medicine",
author = "{van Wielink}, R.",
note = "WU thesis 5332",
year = "2012",
language = "English",
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publisher = "s.n.",
school = "Wageningen University",

}

van Wielink, R 2012, 'Cell culture based production of avian influenza vaccines', Doctor of Philosophy, Wageningen University, S.l..

Cell culture based production of avian influenza vaccines. / van Wielink, R.

S.l. : s.n., 2012. 141 p.

Research output: Thesisinternal PhD, WU

TY - THES

T1 - Cell culture based production of avian influenza vaccines

AU - van Wielink, R.

N1 - WU thesis 5332

PY - 2012

Y1 - 2012

N2 - Vaccination of poultry can be used as a tool to control outbreaks of avian influenza, including that of highly pathogenic H5 and H7 strains. Influenza vaccines are traditionally produced in embryonated chicken eggs. Continuous cell lines have been suggested as an alternative substrate to produce influenza vaccines, as they are more robust and lack the long lead times associated with the production of large quantities of embryonated eggs. In the study that is described in this thesis, the production of influenza virus in cell culture was explored. Therefore, several cell lines were assessed for their ability to propagate influenza virus. Furthermore, adaptations to both cell line and seed virus were suggested that increased virus yield, thereby allowing the production of attenuated influenza virus strains.

AB - Vaccination of poultry can be used as a tool to control outbreaks of avian influenza, including that of highly pathogenic H5 and H7 strains. Influenza vaccines are traditionally produced in embryonated chicken eggs. Continuous cell lines have been suggested as an alternative substrate to produce influenza vaccines, as they are more robust and lack the long lead times associated with the production of large quantities of embryonated eggs. In the study that is described in this thesis, the production of influenza virus in cell culture was explored. Therefore, several cell lines were assessed for their ability to propagate influenza virus. Furthermore, adaptations to both cell line and seed virus were suggested that increased virus yield, thereby allowing the production of attenuated influenza virus strains.

KW - aviaire influenza

KW - aviaire influenza a-virussen

KW - celcultuur vaccins

KW - vaccins

KW - celkweek

KW - virologie

KW - bioproceskunde

KW - veeartsenijkunde

KW - avian influenza

KW - avian influenza a viruses

KW - cell culture vaccines

KW - vaccines

KW - cell culture

KW - virology

KW - bioprocess engineering

KW - veterinary medicine

M3 - internal PhD, WU

SN - 9789461733535

PB - s.n.

CY - S.l.

ER -

van Wielink R. Cell culture based production of avian influenza vaccines. S.l.: s.n., 2012. 141 p.