Abstract
Vaccination of poultry can be used as a tool to control outbreaks of avian influenza, including that of highly pathogenic H5 and H7 strains. Influenza vaccines are traditionally produced in embryonated chicken eggs. Continuous cell lines have been suggested as an alternative substrate to produce influenza vaccines, as they are more robust and lack the long lead times associated with the production of large quantities of embryonated eggs. In the study that is described in this thesis, the production of influenza virus in cell culture was explored. Therefore, several cell lines were assessed for their ability to propagate influenza virus. Furthermore, adaptations to both cell line and seed virus were suggested that increased virus yield, thereby allowing the production of attenuated influenza virus strains.
| Original language | English |
|---|---|
| Qualification | Doctor of Philosophy |
| Awarding Institution |
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| Supervisors/Advisors |
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| Award date | 19 Oct 2012 |
| Place of Publication | S.l. |
| Publisher | |
| Print ISBNs | 9789461733535 |
| Publication status | Published - 2012 |
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Keywords
- avian influenza
- avian influenza a viruses
- cell culture vaccines
- vaccines
- cell culture
- virology
- bioprocess engineering
- veterinary medicine
Cite this
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Cell culture based production of avian influenza vaccines. / van Wielink, R.
S.l. : s.n., 2012. 141 p.Research output: Thesis › internal PhD, WU
TY - THES
T1 - Cell culture based production of avian influenza vaccines
AU - van Wielink, R.
N1 - WU thesis 5332
PY - 2012
Y1 - 2012
N2 - Vaccination of poultry can be used as a tool to control outbreaks of avian influenza, including that of highly pathogenic H5 and H7 strains. Influenza vaccines are traditionally produced in embryonated chicken eggs. Continuous cell lines have been suggested as an alternative substrate to produce influenza vaccines, as they are more robust and lack the long lead times associated with the production of large quantities of embryonated eggs. In the study that is described in this thesis, the production of influenza virus in cell culture was explored. Therefore, several cell lines were assessed for their ability to propagate influenza virus. Furthermore, adaptations to both cell line and seed virus were suggested that increased virus yield, thereby allowing the production of attenuated influenza virus strains.
AB - Vaccination of poultry can be used as a tool to control outbreaks of avian influenza, including that of highly pathogenic H5 and H7 strains. Influenza vaccines are traditionally produced in embryonated chicken eggs. Continuous cell lines have been suggested as an alternative substrate to produce influenza vaccines, as they are more robust and lack the long lead times associated with the production of large quantities of embryonated eggs. In the study that is described in this thesis, the production of influenza virus in cell culture was explored. Therefore, several cell lines were assessed for their ability to propagate influenza virus. Furthermore, adaptations to both cell line and seed virus were suggested that increased virus yield, thereby allowing the production of attenuated influenza virus strains.
KW - aviaire influenza
KW - aviaire influenza a-virussen
KW - celcultuur vaccins
KW - vaccins
KW - celkweek
KW - virologie
KW - bioproceskunde
KW - veeartsenijkunde
KW - avian influenza
KW - avian influenza a viruses
KW - cell culture vaccines
KW - vaccines
KW - cell culture
KW - virology
KW - bioprocess engineering
KW - veterinary medicine
M3 - internal PhD, WU
SN - 9789461733535
PB - s.n.
CY - S.l.
ER -